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. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: Development. 2008 Apr 9;135(10):1781–1790. doi: 10.1242/dev.013847

Figure 6. MALSTKO embryos display significant differences in labeling index and quit fraction during early neurogenesis.

Figure 6

Coronal sections of E11.5 (A,B) and E12.5 (D,E) MALSTKO mutants and matching littermate control brains injected with BrdU 2 hours (A,B,G,H) or 24 hours (D,E) prior to sacrifice. Sections were immunostained with antibodies to BrdU (A,C,D,E,G,H) and Ki67 (D,E). (C) At E11.5, MALSTKO progenitors have a lower labeling index (total number of BrdU+ cells / total number of cells) relative to controls. (F) A larger number of MALSTKO mutant progenitors at E12.5 have quit the cell cycle relative to control littermates (BrdU+Ki67−/ total number of BrdU+ cells). (I) MALSTKO mutant progenitor cells recover normal cell cycle dynamics just a day later at E13.5, at which time no differences in labeling index are apparent. Scale bar 10 μm.