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. 1974 Oct;54(4):935–947. doi: 10.1172/JCI107834

Osmotic Extraction of Hypotonic Fluid from the Lungs

Richard M Effros 1
PMCID: PMC301634  PMID: 4430723

Abstract

After injections of sucrose, NaCl, and urea solutions, the flow of tissue fluid from the lungs amounted to 0.182, 0.216, and 0.152 × 10-3 ml/s per mosmol/kg of concentration difference between plasma and tissues in each gram of wet tissue weight. The extracted fluid contained less than 20% of the Na+, K+ and urea concentrations of the plasma. It was concluded that this fluid was distinctly hypotonic in comparision with the fluids of the plasma and tissue compartments both before and after the injection of hypertonic solutions.

The presence of low solute concentrations in the extracted fluid is attributed to the passage of this fluid across cellular membranes, which are relatively impermeable to small hydrophilic solutes. Movement of fluid out of the junctions appears to be less than that through the endothelial cells. It is suggested that the injected solutes rapidly leak into the junctions and consequently induce relatively little movement of water or tissue solutes out of the junctions. Concentrations of tritiated water and [14C]antipyrine in the extracted fluid are essentially the same as base-line plasma concentrations when the animals have been primed with these tracers. It is therefore likely that these tracers can readily traverse cellular membranes.

Red cell transit through the lungs is impaired by hypertonic solutions of sucrose and NaCl with transient increases in pulmonary arterial hemoglobin concentrations of as much as 35% of base-line values.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHINARD F. P., ENNS T. Transcapillary pulmonary exchange of water in the dog. Am J Physiol. 1954 Aug;178(2):197–202. doi: 10.1152/ajplegacy.1954.178.2.197. [DOI] [PubMed] [Google Scholar]
  2. CRONE C. THE PERMEABILITY OF CAPILLARIES IN VARIOUS ORGANS AS DETERMINED BY USE OF THE 'INDICATOR DIFFUSION' METHOD. Acta Physiol Scand. 1963 Aug;58:292–305. doi: 10.1111/j.1748-1716.1963.tb02652.x. [DOI] [PubMed] [Google Scholar]
  3. Effros R. M. Impairment of red cell transit through the canine lungs following injections of hypertonic fluids. Circ Res. 1972 Oct;31(4):590–601. doi: 10.1161/01.res.31.4.590. [DOI] [PubMed] [Google Scholar]
  4. FEISAL K. A., SONI J., DUBOIS A. B. Pulmonary arterial circulation time, pulmonary arterial blood volume, and the ratio of gas to tissue volume in the lungs of dogs. J Clin Invest. 1962 Feb;41:390–400. doi: 10.1172/JCI104493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fenstermacher J. D., Johnson J. A. Filtration and reflection coefficients of the rabbit blood-brain barrier. Am J Physiol. 1966 Aug;211(2):341–346. doi: 10.1152/ajplegacy.1966.211.2.341. [DOI] [PubMed] [Google Scholar]
  6. Franz T. J., Van Bruggen J. T. Hyperosmolarity and the net transport of nonelectrolytes in frog skin. J Gen Physiol. 1967 Mar;50(4):933–949. doi: 10.1085/jgp.50.4.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guyton A. C., Granger H. J., Taylor A. E. Interstitial fluid pressure. Physiol Rev. 1971 Jul;51(3):527–563. doi: 10.1152/physrev.1971.51.3.527. [DOI] [PubMed] [Google Scholar]
  8. Patlak C. S., Rapoport S. I. Theoretical analysis of net tracer flux due to volume circulation in a membrane with pores of different sizes. Relation to solute drag model. J Gen Physiol. 1971 Feb;57(2):113–124. doi: 10.1085/jgp.57.2.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Perl W. Modified filtration-permeability model of transcapillary transport--a solution of the Pappenheimer pore puzzle? Microvasc Res. 1971 Jul;3(3):233–251. doi: 10.1016/0026-2862(71)90051-3. [DOI] [PubMed] [Google Scholar]
  10. Pietra G. G., Szidon J. P., Leventhal M. M., Fishman A. P. Hemoglobin as a tracer in hemodynamic pulmonary edema. Science. 1969 Dec 26;166(3913):1643–1646. doi: 10.1126/science.166.3913.1643. [DOI] [PubMed] [Google Scholar]
  11. READ R. C., JOHNSON J. A., VICK J. A., MEYER M. W. Vascular effects of hypertonic solutions. Circ Res. 1960 May;8:538–548. doi: 10.1161/01.res.8.3.538. [DOI] [PubMed] [Google Scholar]
  12. RENKIN E. M. Transport of potassium-42 from blood to tissue in isolated mammalian skeletal muscles. Am J Physiol. 1959 Dec;197:1205–1210. doi: 10.1152/ajplegacy.1959.197.6.1205. [DOI] [PubMed] [Google Scholar]
  13. Rand P. W., Lacombe E. Effects of angiocardiographic injections on blood viscosity. Radiology. 1965 Dec;85(6):1022–1032. doi: 10.1148/85.6.1022. [DOI] [PubMed] [Google Scholar]
  14. SHIRLEY H. H., Jr, WOLFRAM C. G., WASSERMAN K., MAYERSON H. S. Capillary permeability to macromolecules: stretched pore phenomenon. Am J Physiol. 1957 Aug;190(2):189–193. doi: 10.1152/ajplegacy.1957.190.2.189. [DOI] [PubMed] [Google Scholar]
  15. Siegwart B., Gehr P., Gil J., Weibel E. R. Morphometric estimation of pulmonary diffusion capacity. IV. The normal dog lung. Respir Physiol. 1971 Nov;13(2):141–159. doi: 10.1016/0034-5687(71)90086-7. [DOI] [PubMed] [Google Scholar]
  16. Solomon A. K. Characterization of biological membranes by equivalent pores. J Gen Physiol. 1968 May;51(5 Suppl):335S+–335S+. [PubMed] [Google Scholar]
  17. Starling E. H. On the Absorption of Fluids from the Connective Tissue Spaces. J Physiol. 1896 May 5;19(4):312–326. doi: 10.1113/jphysiol.1896.sp000596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taylor A. E., Gaar K. A., Jr Estimation of equivalent pore radii of pulmonary capillary and alveolar membranes. Am J Physiol. 1970 Apr;218(4):1133–1140. doi: 10.1152/ajplegacy.1970.218.4.1133. [DOI] [PubMed] [Google Scholar]
  19. Ussing H. H. Anomalous transport of electrolytes and sucrose through the isolated frog skin induced by hypertonicity of the outside bathing solution. Ann N Y Acad Sci. 1966 Jul 14;137(2):543–555. doi: 10.1111/j.1749-6632.1966.tb50180.x. [DOI] [PubMed] [Google Scholar]
  20. Ussing H. H., Johansen B. Anomalous transport of sucrose and urea in toad skin. Nephron. 1969;6(3):317–328. doi: 10.1159/000179736. [DOI] [PubMed] [Google Scholar]
  21. VARGAS F., JOHNSON J. A. AN ESTIMATE OF REFLECTION COEFFICIENTS FOR RABBIT HEART CAPILLARIES. J Gen Physiol. 1964 Mar;47:667–677. doi: 10.1085/jgp.47.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wangensteen O. D., Wittmers L. E., Jr, Johnson J. A. Permeability of the mammalian blood-gas barrier and its components. Am J Physiol. 1969 Apr;216(4):719–727. doi: 10.1152/ajplegacy.1969.216.4.719. [DOI] [PubMed] [Google Scholar]
  23. Yudilevich D. L., Alvarez O. A. Water, sodium, and thiourea transcapillary diffusion in the dog heart. Am J Physiol. 1967 Aug;213(2):308–314. doi: 10.1152/ajplegacy.1967.213.2.308. [DOI] [PubMed] [Google Scholar]
  24. Yudilevich D. L., Renkin E. M., Alvarez O. A., Bravo I. Fractional extraction and transcapillary exchange during continuous and instantaneous tracer administration. Circ Res. 1968 Aug;23(2):325–336. doi: 10.1161/01.res.23.2.325. [DOI] [PubMed] [Google Scholar]

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