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. 1974 Nov;54(5):1241–1244. doi: 10.1172/JCI107868

The Effect of 3′,5′-Adenosine Monophosphate on Granulocyte Adhesion

Richard E Bryant 1,2, Marilyn C Sutgliffe 1,2
PMCID: PMC301672  PMID: 4370901

Abstract

Human granulocyte adhesion to glass capillary tubes was tested in the presence of agents that increase intracellular levels of cyclic 3′,5′-adenosine monophosphate (cAMP). Adhesion was significantly reduced by 10-3-10-4 M dibutyryl cAMP, 10-4-10-6 M prostaglandin E1 (PGE1), 10-4-10-6 M histamine, or 10-3 M theophylline. Adhesion was not suppressed by 10-4 M theophylline unless it was combined with PGE1 or histamine. Eosinophil and basophil adhesion was especially sensitive to suppression by the above agents. These findings suggest that intracellular cAMP may play a role in regulation of adhesiveness of human basophils, eosinophils, and neutrophils.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON F., Jr, SMITH M. R., WOOD W. B., Jr Studies on the pathogenesis of acute inflammation. II. The action of cortisone on the inflammatory response to thermal injury. J Exp Med. 1955 Dec 1;102(6):669–676. doi: 10.1084/jem.102.6.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bourne H. R., Lichtenstein L. M., Melmon K. L. Pharmacologic control of allergic histamine release in vitro: evidence for an inhibitory role of 3',5'-adenosine monophosphate in human leukocytes. J Immunol. 1972 Mar;108(3):695–705. [PubMed] [Google Scholar]
  3. Bryant R. E., Des Prez R. M., Rogers D. E. Studies on human leukocyte motility. II. Effects of bacterial endotoxin on leukocyte migration, adhesiveness, and aggregation. Yale J Biol Med. 1967 Dec;40(3):192–204. [PMC free article] [PubMed] [Google Scholar]
  4. Bryant R. E., DesPrez R. M., VanWay M. H., Rogers D. E. Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation. J Exp Med. 1966 Sep 1;124(3):483–499. doi: 10.1084/jem.124.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryant R. E., Sutcliffe M. C. A method for quantitation of human leukocyte adhesion to glass. Proc Soc Exp Biol Med. 1972 Oct;141(1):196–202. doi: 10.3181/00379727-141-36741. [DOI] [PubMed] [Google Scholar]
  6. Bryant R. E., Sutcliffe M. C., McGee Z. A. Effect of osmolalities comparable to those of the renal medulla on function of human polymorphonuclear leukocytes. J Infect Dis. 1972 Jul;126(1):1–10. doi: 10.1093/infdis/126.1.1. [DOI] [PubMed] [Google Scholar]
  7. COHN Z. A., MORSE S. I. Functional and metabolic properties of polymorphonuclear leucocytes. I. Observations on the requirements and consequences of particle ingestion. J Exp Med. 1960 May 1;111:667–687. doi: 10.1084/jem.111.5.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. COHN Z. A., MORSE S. I. Functional and metabolic properties of polymorphonuclear leucocytes. II. The influence of a lipopolysaccharide endotoxin. J Exp Med. 1960 May 1;111:689–704. doi: 10.1084/jem.111.5.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cooper J. R., Cruickshank C. N. Improved method for direct counting of basophil leucocytes. J Clin Pathol. 1966 Jul;19(4):402–402. doi: 10.1136/jcp.19.4.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ignarro L. J. Neutral protease release from human leukocytes regulated by neurohormones and cyclic nucleotides. Nat New Biol. 1973 Oct 3;245(144):151–154. doi: 10.1038/newbio245151a0. [DOI] [PubMed] [Google Scholar]
  11. Salzman E. W., Kensler P. C., Levine L. Cyclic 3',5'-adenosine monophosphate in human blood platelets. IV. Regulatory role of cyclic amp in platelet function. Ann N Y Acad Sci. 1972 Oct 27;201:61–71. doi: 10.1111/j.1749-6632.1972.tb16287.x. [DOI] [PubMed] [Google Scholar]
  12. Tse R. L., Phelps P., Urban D. Polymorphonuclear leukocyte motility in vitro. VI. Effect of purine and pyrimidine analogues: possible role of cyclic AMP. J Lab Clin Med. 1972 Aug;80(2):264–274. [PubMed] [Google Scholar]
  13. WOOD W. B., Jr Studies on the cellular immunology of acute bacterial infections. Harvey Lect. 1951;Series 47:72–98. [PubMed] [Google Scholar]
  14. Zurier R. B., Weissmann G., Hoffstein S., Kammerman S., Tai H. H. Mechanisms of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function. J Clin Invest. 1974 Jan;53(1):297–309. doi: 10.1172/JCI107550. [DOI] [PMC free article] [PubMed] [Google Scholar]

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