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. 1975 Jan;55(1):198–200. doi: 10.1172/JCI107912

The effect of Tuftsin on the nitrous blue tetrazolium reduction of normal human polymorphonuclear leukocytes.

Z Spirer, V Zakuth, A Golander, N Bogair, M Fridkin
PMCID: PMC301733  PMID: 1172670

Abstract

The influence of Tuftsin, the synthetic phagocytosis-stimulating tetrapeptide (L-threonyl-L-lysyl-L-prolyl-L-arginine), on the nitrous blue tetrazolium (NBT) reduction by human polymorphonuclear leukocytes was investigated. It was found that this substance increases the NBT reduction by approximately as much as endotoxin. Other tetrapeptides do not share this property. When Tuftsin analogs are added to the cell suspension and incubated, they prevent the action of both Tuftsin and endotoxin but not of methylene blue. When washed of the analogs, the cells regain the property to be activated by both Tuftsin and endotoxin. It appears that methylene blue on one hand and Tuftsin and endotoxin on the other hand have different sites for their actions. We suggest that whereas methylene blue diffuses into the cell and acts directly upon the hexosemonophosphate shunt activation, Tuftsin and endotoxin appear to act on the cell membrane binding to specific receptors. By treating the cells with Tuftsin analogs, we probably block these receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baehner R. L., Nathan D. G. Quantitative nitroblue tetrazolium test in chronic granulomatous disease. N Engl J Med. 1968 May 2;278(18):971–976. doi: 10.1056/NEJM196805022781801. [DOI] [PubMed] [Google Scholar]
  2. Constantopoulos A., Najjar V. A., Smith J. W. Tuftsin deficiency: a new syndrome with defective phagocytosis. J Pediatr. 1972 Apr;80(4):564–572. doi: 10.1016/s0022-3476(72)80051-9. [DOI] [PubMed] [Google Scholar]
  3. Constantopoulos A., Najjar V. A., Wish J. B., Necheles T. H., Stolbach L. L. Defective phagocytosis due to tuftsin deficiency in splenectomized subjects. Am J Dis Child. 1973 May;125(5):663–665. doi: 10.1001/archpedi.1973.04160050017004. [DOI] [PubMed] [Google Scholar]
  4. Fidalgo B. V., Najjar V. A. The physiological role of the lymphoid system, 3. Leucophilic gamma-globulin and the phagocytic activity of the polymorphonuclear leucocyte. Proc Natl Acad Sci U S A. 1967 Apr;57(4):957–964. doi: 10.1073/pnas.57.4.957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fidalgo B. V., Najjar V. A. The physiological role of the lymphoid system. VI. The stimulatory effect of leucophilic gamma globulin (leucokinin) on the phagocytic activity of human polymorphonuclear leucocyte. Biochemistry. 1967 Nov;6(11):3386–3392. doi: 10.1021/bi00863a008. [DOI] [PubMed] [Google Scholar]
  6. Humbert J. R., Gross G. P., Vatter A. E., Hathaway W. E. Nitroblue tetrazolium reduction by neutrophils: biochemical and ultrastructural effects of methylene blue. J Lab Clin Med. 1973 Jul;82(1):20–30. [PubMed] [Google Scholar]
  7. Karnovsky M. L. The metabolism of leukocytes. Semin Hematol. 1968 Apr;5(2):156–165. [PubMed] [Google Scholar]
  8. Merrifield R. B. Solid-phase peptide synthesis. Adv Enzymol Relat Areas Mol Biol. 1969;32:221–296. doi: 10.1002/9780470122778.ch6. [DOI] [PubMed] [Google Scholar]
  9. Najjar V. A., Constantopoulos A. A new phagocytosis-stimulating tetrapeptide hormone, tuftsin, and its role in disease. J Reticuloendothel Soc. 1972 Aug;12(2):197–215. [PubMed] [Google Scholar]
  10. Najjar V. A., Fidalgo B. V., Stitt E. The physiological role of the lymphoid system. VII. The disappearance of leucokinin activity following splenectomy. Biochemistry. 1968 Jun;7(6):2376–2379. doi: 10.1021/bi00846a046. [DOI] [PubMed] [Google Scholar]
  11. Najjar V. A., Nishioka K. "Tuftsin": a natural phagocytosis stimulating peptide. Nature. 1970 Nov 14;228(5272):672–673. doi: 10.1038/228672a0. [DOI] [PubMed] [Google Scholar]
  12. Nishioka K., Constantopoulos A., Satoh P. S., Najjar V. A. The characteristics, isolation and synthesis of the phagocytosis stimulating peptide tuftsin. Biochem Biophys Res Commun. 1972 Apr 14;47(1):172–179. doi: 10.1016/s0006-291x(72)80025-1. [DOI] [PubMed] [Google Scholar]
  13. Park B. H., Fikrig S. M., Smithwick E. M. Infection and nitroblue-tetrazolium reduction by neutrophils. A diagnostic acid. Lancet. 1968 Sep 7;2(7567):532–534. doi: 10.1016/s0140-6736(68)92406-9. [DOI] [PubMed] [Google Scholar]
  14. Park H. H., Good R. A. N.B.T. test stimulated. Lancet. 1970 Sep 19;2(7673):616–616. doi: 10.1016/s0140-6736(70)90207-2. [DOI] [PubMed] [Google Scholar]
  15. Segal A. W., Levi A. J. The mechanism of the entry of dye into neutrophils in the nitroblue tetrazolium (NBT) test. Clin Sci Mol Med. 1973 Dec;45(6):817–826. doi: 10.1042/cs0450817. [DOI] [PubMed] [Google Scholar]

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