Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1975 Feb;55(2):419–426. doi: 10.1172/JCI107946

Hepatocellular uptake of taurocholate in the dog.

S Erlinger
PMCID: PMC301761  PMID: 1127108

Abstract

The purpose of this study was to examine the hepatocellular extraction of taurocholate and to determine the kinetic characteristics of the uptake process. The uptake of taurocholate by the liver of the intact dog was studied by the multiple-indicator dilution method. 51Cr-labeled red blood cells (a vascular indicator), 125I-labeled albumin (an extravascular reference), and [14C]taurocholate were injected into the portal vein. Different doses of unlabeled taurocholate were included in the injection mixture. Hepatic venous dilution curves were obtained. As a consequence of the hepatic uptake, the outflow recovery of [14C]taurocholate was much reduced when compared to that of albumin, but its recovery increased with increasing doses of taurocholate, suggesting a progressive saturation of the uptake process. The analysis of the dilution curves fitted a three-compartment model system well and no return of the extracted taurocholate to the extracellular space could be detected. The initial space of distribution of taurocholate was 1.22 plus or minus 0.12 (SD) times greater than that of albumin. Analysis of the data for uptake was consistent with Michaelis-Menten kinetics. The calculated initial maximal velocity of uptake (Vmax) was 4.53 mumol times s--1 times 100 g of liver--1 and the dose yielding half-maximal velocity (DK) was 7.11 mumol times 100 g of liver--1. These results are consistent with the hypothesis that the uptake of taurocholate is carrier-mediated. The maximal vilocity of uptake was about six times the known maximal capacity of biliary secretion of taurocholate in the dog.

Full text

PDF
419

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker K. J., Bradley S. E. Binding of sulfobromophthalein (BSP) sodium by plasma albumin. Its role in hepatic BSP extraction. J Clin Invest. 1966 Feb;45(2):281–287. doi: 10.1172/JCI105341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bravo I. R., Yudilevich D. L. Liver distribution and uptake of molecules studied by rapid indicator-dilution technique. Am J Physiol. 1971 Nov;221(5):1449–1455. doi: 10.1152/ajplegacy.1971.221.5.1449. [DOI] [PubMed] [Google Scholar]
  3. GORESKY C. A. A linear method for determining liver sinusoidal and extravascular volumes. Am J Physiol. 1963 Apr;204:626–640. doi: 10.1152/ajplegacy.1963.204.4.626. [DOI] [PubMed] [Google Scholar]
  4. GORESKY C. A. INITIAL DISTRIBUTION AND RATE OF UPTAKE OF SULFOBROMOPHTHALEIN IN THE LIVER. Am J Physiol. 1964 Jul;207:13–26. doi: 10.1152/ajplegacy.1964.207.1.13. [DOI] [PubMed] [Google Scholar]
  5. GORESKY C. A., SILVERMAN M. EFFECT OF CORRECTION OF CATHETER DISTORTION ON CALCULATED LIVER SINUSOIDAL VOLUMES. Am J Physiol. 1964 Oct;207:883–892. doi: 10.1152/ajplegacy.1964.207.4.883. [DOI] [PubMed] [Google Scholar]
  6. Goresky C. A., Bach G. C., Nadeau B. E. On the uptake of materials by the intact liver. The concentrative transport of rubidium-86. J Clin Invest. 1973 May;52(5):975–990. doi: 10.1172/JCI107299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goresky C. A., Bach G. G., Nadeau B. E. On the uptake of materials by the intact liver. The transport and net removal of galactose. J Clin Invest. 1973 May;52(5):991–1009. doi: 10.1172/JCI107300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levi A. J., Gatmaitan Z., Arias I. M. Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J Clin Invest. 1969 Nov;48(11):2156–2167. doi: 10.1172/JCI106182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MEIER P., ZIERLER K. L. On the theory of the indicator-dilution method for measurement of blood flow and volume. J Appl Physiol. 1954 Jun;6(12):731–744. doi: 10.1152/jappl.1954.6.12.731. [DOI] [PubMed] [Google Scholar]
  10. O'Máille E. R., Richards T. G., Short A. H. Acute taurine depletion and maximal rates of hepatic conjugation and secretion of cholic acid in the dog. J Physiol. 1965 Sep;180(1):67–79. [PMC free article] [PubMed] [Google Scholar]
  11. O'Máille E. R., Richards T. G., Short A. H. Observations on the elimination rates of single injections of taurocholate and cholate in the dog. Q J Exp Physiol Cogn Med Sci. 1969 Jul;54(3):296–310. doi: 10.1113/expphysiol.1969.sp002028. [DOI] [PubMed] [Google Scholar]
  12. O'Máille E. R., Richards T. G., Short A. H. The influence of conjugation of cholic acid on its uptake and secretion: hepatic extraction of taurocholate and cholate in the dog. J Physiol. 1967 Apr;189(2):337–350. doi: 10.1113/jphysiol.1967.sp008172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PRATT E. B., BURDICK F. D., HOLMES J. H. Measurement of liver blood flow in unanesthetized dog using the bromsulfalein dye method. Am J Physiol. 1952 Nov;171(2):471–478. doi: 10.1152/ajplegacy.1952.171.2.471. [DOI] [PubMed] [Google Scholar]
  14. RUDMAN D., KENDALL F. E. Bile acid content of human serum. II. The binding of cholanic acids by human plasma proteins. J Clin Invest. 1957 Apr;36(4):538–542. doi: 10.1172/JCI103451. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES