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HEK 293, MIA PaCa2 and HPDE cells stably expressing PDX-1 with and without human PDX-1 shRNA had increased cell proliferation. PDX-1 overexpression caused significant increase of cell proliferation in all cell lines as shown in A, B and C. PDX-1 shRNA co-transfection was able to reverse the effect induced by PDX-1.
