Skip to main content
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1975 Mar;55(3):655–660. doi: 10.1172/JCI107973

Sodium- and potassium-activated ATPase. A possible target of aldosterone.

U Schmidt, J Schmid, H Schmid, U C Dubach
PMCID: PMC301794  PMID: 123253

Abstract

Na-K-ATPase activity was measured with an ultramicromethod in single portions of the proximal and distal convolution and of the thick ascending limb of Henle from adrenalectomized rats and after treatment with 5 mug aldosterone per 100 g body wt. The activity in all tubular structures returned to normal within 1 h after injection. This rapid activation of Na-K-ATPase induced by hormone was completely prevented by actinomycin D and cycloheximide. It appears that this aldosterone effect on Na-K-ATPase requires an intact protein synthetic process.

Full text

PDF
655

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burg M. B., Green N. Function of the thick ascending limb of Henle's loop. Am J Physiol. 1973 Mar;224(3):659–668. doi: 10.1152/ajplegacy.1973.224.3.659. [DOI] [PubMed] [Google Scholar]
  2. Chu L. L., Edelman I. S. Cordycepin and alpha-amanitin: inhibitors of transcription as probes of aldosterone action. J Membr Biol. 1972 Dec 29;10(3):291–310. doi: 10.1007/BF01867862. [DOI] [PubMed] [Google Scholar]
  3. Goodman D. B., Allen J. E., Rasmussen H. Studies on the mechanism of action of aldosterone: hormone-induced changes in lipid metabolism. Biochemistry. 1971 Oct 12;10(21):3825–3831. doi: 10.1021/bi00797a004. [DOI] [PubMed] [Google Scholar]
  4. Hash J. H. Antibiotic mechanisms. Annu Rev Pharmacol. 1972;12:35–56. doi: 10.1146/annurev.pa.12.040172.000343. [DOI] [PubMed] [Google Scholar]
  5. Hendler E. D., Torretti J., Kupor L., Epstein F. H. Effects of adrenalectomy and hormone replacement on Na- K-ATPase in renal tissue. Am J Physiol. 1972 Mar;222(3):754–760. doi: 10.1152/ajplegacy.1972.222.3.754. [DOI] [PubMed] [Google Scholar]
  6. Hierholzer K., Stolte H. The proximal and distal tubular action of adrenal steroids on Na reabsorption. Nephron. 1969;6(3):188–204. doi: 10.1159/000179728. [DOI] [PubMed] [Google Scholar]
  7. Hill J. H., Cortas N., Walser M. Aldosterone action and sodium- and potassium-activated adenosine triphosphatase in toad bladder. J Clin Invest. 1973 Jan;52(1):185–189. doi: 10.1172/JCI107163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jorgensen P. L. Regulation of the (Na+ equals K+)-activated ATP hydrolyzing enzyme system in rat kidney. II. The effect of aldosterone on the activity in kidneys of adrenalectomized rats. Biochim Biophys Acta. 1969 Nov 18;192(2):326–334. [PubMed] [Google Scholar]
  9. Jorgensen P. L., Skou J. C. Preparation of highly active (Na+ + K+)-ATPase from the outer medulla of rabbit kidney. Biochem Biophys Res Commun. 1969 Sep 24;37(1):39–46. doi: 10.1016/0006-291x(69)90877-8. [DOI] [PubMed] [Google Scholar]
  10. Jorgensen P. L. The role of aldosterone in the regulation of (Na + + K + )-ATPase in rat kidney. J Steroid Biochem. 1972 Feb;3(2):181–191. doi: 10.1016/0022-4731(72)90049-0. [DOI] [PubMed] [Google Scholar]
  11. Kirsten R., Kirsten E. Redox state of pyridine nucleotides in renal response to aldosterone. Am J Physiol. 1972 Jul;223(1):229–235. doi: 10.1152/ajplegacy.1972.223.1.229. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H. The quantitative histochemistry of the brain; histological sampling. J Histochem Cytochem. 1953 Nov;1(6):420–428. doi: 10.1177/1.6.420. [DOI] [PubMed] [Google Scholar]
  13. Lahav M., Dietz T., Edelman I. S. The action of aldosterone on sodium transport: further studies with inhibitors of RNA and protein synthesis. Endocrinology. 1973 Jun;92(6):1685–1699. doi: 10.1210/endo-92-6-1685. [DOI] [PubMed] [Google Scholar]
  14. Landon E. J., Jazab N., Forte L. Aldosterone and sodium-potassium-dependent ATPase activity of rat kidney membranes. Am J Physiol. 1966 Oct;211(4):1050–1056. doi: 10.1152/ajplegacy.1966.211.4.1050. [DOI] [PubMed] [Google Scholar]
  15. Ling G. N., Miller C., Ochsenfeld M. M. The physical state of solutes and water in living cells according to the association-induction hypothesis. Ann N Y Acad Sci. 1973 Mar 30;204:6–50. doi: 10.1111/j.1749-6632.1973.tb30770.x. [DOI] [PubMed] [Google Scholar]
  16. Lynch R. E., Schneider E. G., Willis L. R., Knox F. G. Absence of mineralocorticoid-dependent sodium reabsorption in dog proximal tubule. Am J Physiol. 1972 Jul;223(1):40–45. doi: 10.1152/ajplegacy.1972.223.1.40. [DOI] [PubMed] [Google Scholar]
  17. Rea C., Segal S. ATP content of rat kidney cortex slices: relation to alpha-aminoisobutyric acid uptake. Kidney Int. 1972 Aug;2(2):101–106. doi: 10.1038/ki.1972.77. [DOI] [PubMed] [Google Scholar]
  18. Sakai F., Murayama Y. Effects of aldosterone and cortisol of Henle's loop in the adrenalectomized rats kidney. Jpn J Pharmacol. 1971 Feb;21(1):23–31. doi: 10.1254/jjp.21.23. [DOI] [PubMed] [Google Scholar]
  19. Schmidt U., Dubach U. C. Activity of (Na+K+)-stimulated adenosintriphosphatase in the rat nephron. Pflugers Arch. 1969;306(3):219–226. doi: 10.1007/BF00592433. [DOI] [PubMed] [Google Scholar]
  20. Schmidt U., Dubach U. C. Na K stimulated adenosinetriphosphatase: intracellular localisation within the proximal tubule of the rat nephron. Pflugers Arch. 1971;330(3):265–270. doi: 10.1007/BF00588617. [DOI] [PubMed] [Google Scholar]
  21. Schmidt U., Dubach U. C. Sensitivity of Na K adenosine triphosphatase activity in various structures of the rat nephron: studies with adrenalectomy. Eur J Clin Invest. 1971 May;1(5):307–312. doi: 10.1111/j.1365-2362.1971.tb00636.x. [DOI] [PubMed] [Google Scholar]
  22. Schmidt U., Schmid H., Funk B., Dubach U. C. The function of Na, K-ATPase in single portions of the rat nephron. Ann N Y Acad Sci. 1974;242(0):489–500. doi: 10.1111/j.1749-6632.1974.tb19112.x. [DOI] [PubMed] [Google Scholar]
  23. Stolte H., Brecht J. P., Wiederholt M., Hierholzer K. Einfluss von Adrenalektomie und Glucocorticoiden auf die Wasserpermeabilität corticaler Nephronabschnitte der Rattenniere. Pflugers Arch Gesamte Physiol Menschen Tiere. 1968;299(2):99–127. [PubMed] [Google Scholar]
  24. Wiederholt M., Stolte H., Brecht J. P., Hierholzer K. Mikropunktionsuntersuchungen über den Einfluss von Aldosteron, Cortison und Dexamethason auf die renale Natriumresorption adrenalektomierter Ratten. Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;292(4):316–333. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES