TABLE 3.

Courtship-responsive genes

		Average fold change in courting male heads compared to:
Gene identifier	Gene name	Male–male heads	Control male heads	GO molecular function	GO biological process
CG1416		1.12	1.12	ATPase activator activity	Response to stress
CG1897	Drop (Dr)	1.35	1.44	Sequence-specific DNA binding	Nervous system development
CG3850	sugarbabe (sug)	1.3	1.61	Transcription activator activity	Regulation of transcription
CG6461		1.1	1.1	Transferase activity	Unknown
CG6494	hairy (h)	1.63	1.52	Transcription repressor activity	Nervous system development
CG11877		1.11	1.12	Unknown	Unknown
CG13116		1.21	1.25	Unknown	Unknown
CG14489	olf186-M	1.18	1.27	Unknown	Unknown
CG14548	E(spl) region transcript mβ (HLHmβ)	1.39	1.58	Transcription repressor activity	Nervous system development
CG30445	Tyrosine decarboxyl-ase 1	1.22	1.24	Tyrosine decarboxylase activity	Unknown
CG31137	twin	1.28	1	Transcription regulator activity	Nuclear-transcribed mRNA poly(A) tail shortening
CG4962	CG4962	−1.28	−1.20	Unknown	Unknown
CG14210	CG14210	−1.23	−1.02	Protein binding	Unknown
CG6081	Cyp28d2	−1.11	−1.18	Oxidoreductase activity	Oxidation reduction
CG32491	modifier of mdg4 [mod(mdg4)]	−1.09	−1.16	Transcription factor activity	Induction of apoptosis
CG18525	Serine protease inhibitor 5 (Spn5)	−1.14	−1.11	Serine-type endopeptidase inhibitor activity	Unknown

Eleven upregulated and 5 downregulated genes are courtship-responsive when comparing all three treatment groups (control, courting, and male-exposed) by mixed ANOVA and Tukey's post-hoc analyses (P < 0.05).