TABLE 4.

qPCR confirmation of the microarray results

Gene identifier	Gene symbol	Average relative expression level in control male heads ±SEM	Average relative expression level in courting male heads ±SEM	Relative qPCR fold change ±SEM	Relative array fold change ±SEM
CG9377		0.07 ± 0.01	0.09 ± 0.02	1.25 ± 0.25	1.7 ± 0.07
CG10621		1.95 ± 0.46	2.42 ± 0.63	1.31 ± 0.28	1.44 ± 0.03
CG9659	egh	3.2 ± 1.07	6.62 ± 1.72	2.04 ± 0.42	1.36 ± 0.02
CG14548	HLHmβ	0.65 ± 0.16	1.92 ± 0.62	3.13 ± 0.82*	1.58 ± 0.05
CG6806	Lsp2	0.57 ± 0.17	1.5 ± 0.72	2.79 ± 1.09	1.34 ± 0.03
CG3850	sug	3.95 ± 1.2	9.31 ± 2.58	2.36 ± 0.58*	1.6 ± 0.05
CG31181		0.6 ± 0.2	0.15 ± 0.11	−2.7 ± 0.09*	−1.36 ± 0.03
CG33547	Rim	0.4 ± 0.09	0.03 ± 0.004	−12.5 ± 0.006	−1.44 ± 0.06
CG12348	Sh	0.39 ± 0.09	0.12 ± 0.04	−3.13 ± 0.09*	−1.32 ± 0.04

Average relative expression of nine genes was assessed by qPCR. An asterisk indicates a significant (P < 0.05) difference in the average relative expression level in control male heads compared to courting-male heads as determined by qPCR. SEM, standard error of the mean.