Analysis of conditional gene deletion using probe based Real-Time PCR

Abstract

Following publication of this article [1] the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science"). The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows

Probe for 1lox/2lox allele quantification:

5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3'

(uppercase letters = LNA bases)

Manufacturer: EUROGENTEC, Seraing, Belgium

All other information and reaction conditions in the paper are correct as stated.