Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1975 May;55(5):994–1002. doi: 10.1172/JCI108029

A micropuncture study of renal salt and water retention in chronic bile duct obstruction.

N Bank, H S Aynedjian
PMCID: PMC301845  PMID: 1123434

Abstract

The mechanism of sodium retention by the kidney in rats with ligation of the common bile duct was studied with micropuncture techniques. 10-14 days after bile duct ligation, rats showed positive sodium balance and ascites formation. Measurements of renal blood flow and glomerular filtration rate yielded values that were not different from those in normal control animals. Likewise, single nephron filtration rte of surface nephrons was the same in the experimental rats as in the controls. Sodium reabsorption, however, was markedly increased in the proximal convoluted tubule, as well as in segments beyond the proximal convolutions. Single nephron filtration fraction, calculated from measurements of efferent arteriolar and arterial hematocrits, was significantly elevated in the cortical nephrons, even though whole kidney filtration fraction was the same as in normal rats. The calculated protein concentration of cortical peritubular blood was higher in the bile duct-ligated rats than in the normal controls. The observations are consistent with the view that sodium retention is the result of enhanced reabsorption primarily by cortical nephrons. The enhanced reabsorption can be accounted for by relative cortical ischemia due to efferent arteriolar vasoconstriction with the consequent elevation of peritubular colloid oncotic pressure.

Full text

PDF
994

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANK N. Relationship between electrical and hydrogen ion gradients across rat proximal tubule. Am J Physiol. 1962 Sep;203:577–582. doi: 10.1152/ajplegacy.1962.203.3.577. [DOI] [PubMed] [Google Scholar]
  2. Bank N., Aynedjian H. S. A micropuncture study of potassium excretion by the remnant kidney. J Clin Invest. 1973 Jun;52(6):1480–1490. doi: 10.1172/JCI107322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Better O. S., Massry S. G. Effect of chronic bile duct obstruction on renal handling of salt and water. J Clin Invest. 1972 Feb;51(2):402–411. doi: 10.1172/JCI106826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brenner B. M., Galla J. H. Influence of postglomerular hematocrit and protein concentration on rat nephron fluid transfer. Am J Physiol. 1971 Jan;220(1):148–161. doi: 10.1152/ajplegacy.1971.220.1.148. [DOI] [PubMed] [Google Scholar]
  5. Brenner B. M., Troy J. L., Daugharty T. M., MacInnes R. M. Quantitative importance of changes in postglomerular colloid osmotic pressure in mediating glomerulotubular balance in the rat. J Clin Invest. 1973 Jan;52(1):190–197. doi: 10.1172/JCI107164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chaimovitz C., Massry S. G., Friedler R. M., Coburn J. W. Effect of renal denervation and alpha-adrenergic blockade on sodium excretion in dogs with chronic ligation of the common bile duct. Proc Soc Exp Biol Med. 1974 Jul;146(3):764–770. doi: 10.3181/00379727-146-38189. [DOI] [PubMed] [Google Scholar]
  7. Cortney M. A., Mylle M., Lassiter W. E., Gottschalk C. W. Renal tubular transport of water, solute, and PAH in rats loaded with isotonic saline. Am J Physiol. 1965 Dec;209(6):1199–1205. doi: 10.1152/ajplegacy.1965.209.6.1199. [DOI] [PubMed] [Google Scholar]
  8. Epstein M., Berk D. P., Hollenberg N. K., Adams D. F., Chalmers T. C., Abrams H. L., Merrill J. P. Renal failure in the patient with cirrhosis. The role of active vasoconstriction. Am J Med. 1970 Aug;49(2):175–185. doi: 10.1016/s0002-9343(70)80073-0. [DOI] [PubMed] [Google Scholar]
  9. GLABMAN S., AYNEDJIAN H. S., BANK N. MICROPUNCTURE STUDY OF THE EFFECT OF ACUTE REDUCTIONS IN GLOMERULAR FILTRATION RATE ON SODIUM AND WATER REABSORPTION BY THE PROXIMAL TUBULES OF THE RAT. J Clin Invest. 1965 Aug;44:1410–1416. doi: 10.1172/JCI105246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gliedman M. L., Carroll H. J., Popowitz L., Mullane J. F. An experimental hepatorenal syndrome. Surg Gynecol Obstet. 1970 Jul;131(1):34–40. [PubMed] [Google Scholar]
  11. Horster M., Thurau K. Micropuncture studies on the filtration rate of single superficial and juxtamedullary glomeruli in the rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1968;301(2):162–181. doi: 10.1007/BF00362733. [DOI] [PubMed] [Google Scholar]
  12. JICK H., KAMM D. E., SNYDER J. G., MORRISON R. S., CHALMERS T. C. ON THE CONCENTRATING DEFECT IN CIRRHOSIS OF THE LIVER. J Clin Invest. 1964 Feb;43:258–266. doi: 10.1172/JCI104910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jamison R. L. Micropuncture study of superficial and juxtamedullary nephrons in the rat. Am J Physiol. 1970 Jan;218(1):46–55. doi: 10.1152/ajplegacy.1970.218.1.46. [DOI] [PubMed] [Google Scholar]
  14. Kilcoyne M. M., Cannon P. J. Influence of thoracic caval occlusion on intrarenal blood flow distribution and sodium excretion. Am J Physiol. 1971 May;220(5):1220–1230. doi: 10.1152/ajplegacy.1971.220.5.1220. [DOI] [PubMed] [Google Scholar]
  15. Kilcoyne M. M., Cannon P. J. Neural and humoral influences on intrarenal blood flow distribution during thoracic caval occlusion. Am J Physiol. 1971 May;220(5):1231–1237. doi: 10.1152/ajplegacy.1971.220.5.1231. [DOI] [PubMed] [Google Scholar]
  16. Mullane J. F., Gliedman M. L. Renal response to saline load in experimental liver disease. J Surg Res. 1970 Nov;10(11):519–523. doi: 10.1016/0022-4804(70)90077-6. [DOI] [PubMed] [Google Scholar]
  17. Newsome H. H., Jr, Kafka M. S., Bartter F. C. Intrarenal blood flow in dogs with constriction of the inferior thoracic vena cava. Am J Physiol. 1971 Jul;221(1):48–52. doi: 10.1152/ajplegacy.1971.221.1.48. [DOI] [PubMed] [Google Scholar]
  18. Powell L. W., Dunnicliff M. A., Billing B. H. Red cell survival in experimental cholestatic cholestatic jaundice. Br J Haematol. 1968 Nov;15(5):429–435. doi: 10.1111/j.1365-2141.1968.tb01562.x. [DOI] [PubMed] [Google Scholar]
  19. SCHEDL H. P., BARTTER F. C. An explanation for and experimental correction of the abnormal water diuresis in cirrhosis. J Clin Invest. 1960 Feb;39:248–261. doi: 10.1172/JCI104035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SHEAR L., HALL P. W., 3rd, GABUZDA G. J. RENAL FAILURE IN PATIENTS WITH CIRRHOSIS OF THE LIVER. II. FACTORS INFLUENCING MAXIMAL URINARY FLOW RATE. Am J Med. 1965 Aug;39:199–209. doi: 10.1016/0002-9343(65)90042-2. [DOI] [PubMed] [Google Scholar]
  21. SHEAR L., KLEINERMAN J., GABUZDA G. J. RENAL FAILURE IN PATIENTS WITH CIRRHOSIS OF THE LIVER. I. CLINICAL AND PATHOLOGIC CHARACTERISTICS. Am J Med. 1965 Aug;39:184–198. doi: 10.1016/0002-9343(65)90041-0. [DOI] [PubMed] [Google Scholar]
  22. Schrier R. W., De Wardener H. E. Tubular reabsorption of sodium ion: influence of factors other than aldosterone and glomerular filtration rate. 1. N Engl J Med. 1971 Nov;285(22):1231–1243. doi: 10.1056/NEJM197111252852205. [DOI] [PubMed] [Google Scholar]
  23. Schroeder E. T., Shear L., Sancetta S. M., Gabuzda G. J. Renal failure in patients with cirrhosis of the liver. 3. Evaluation of intrarenal blood flow by para-aminohippurate extraction and response to angiotensin. Am J Med. 1967 Dec;43(6):887–896. doi: 10.1016/0002-9343(67)90247-1. [DOI] [PubMed] [Google Scholar]
  24. Seldin D. W., Eknoyan G., Suki W. N., Rector F. C., Jr Localization of diuretic action from the pattern of water and electrolyte excretion. Ann N Y Acad Sci. 1966 Nov 22;139(2):328–343. doi: 10.1111/j.1749-6632.1966.tb41207.x. [DOI] [PubMed] [Google Scholar]
  25. Stumpe K. O., Lowitz H. D., Ochwadt B. Function of juxtamedullary nephrons in normotensive and chronically hypertensive rats. Pflugers Arch. 1969;313(1):43–52. doi: 10.1007/BF00586327. [DOI] [PubMed] [Google Scholar]
  26. WINDHAGER E. E., GIEBISCH G. Micropuncture study of renal tubular transfer of sodium chloride in the rat. Am J Physiol. 1961 Mar;200:581–590. doi: 10.1152/ajplegacy.1961.200.3.581. [DOI] [PubMed] [Google Scholar]
  27. Weinman E. J., Kashgarian M., Hayslett J. P. Role of peritubular protein concentration in sodium reabsorption. Am J Physiol. 1971 Nov;221(5):1521–1528. doi: 10.1152/ajplegacy.1971.221.5.1521. [DOI] [PubMed] [Google Scholar]
  28. Yarger W. E., Aynedjian H. S., Bank N. A micropuncture study of postobstructive diuresis in the rat. J Clin Invest. 1972 Mar;51(3):625–637. doi: 10.1172/JCI106852. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES