Abstract
Intact diaphragms from vitamin D-deficient rats were incubated in vitro with [3H]leucine. Oral administration of 10 mug (400 U) of cholecalciferol 7 h before incubation increased leucine incorporation into diaphragm muscle protein by 136% (P less than 0.001) of the preparation from untreated animals. Nephrectomy did not obliterate this response. ATP content of the diaphragm muscle was also enhanced 7 h after administration of the vitamin. At 4 h after administration of cholecalciferol, serum phosphorus concentration was reduced by 0.7 mg/100 ml (P less than 0.025) and the rate of inorganic 32PO4 accumulation by diaphragm muscle was increased by 18% (P less than 0.025) over the untreated animals. Increasing serum phosphate concentration of the vitamin D-deficient animals by dietary supplementation with phosphate for 3 days failed to significantly enhance leucine incorporation into protein. However, supplementation of the rachitogenic, vitamin D-deficient diet with phosphorus for 3 wk stimulated the growth of the animal and muscle ATP levels. This increase in growth and muscle ATP content attributed to the addition of phosphorus to the diet was less than the increase in growth and muscle ATP levels achieved by the addition of both phosphorus and vitamin D to the diet. To eliminate systemic effects of the vitamin, the epitrochlear muscle of the rat foreleg of vitamin D-depleted rats was maintained in tissue culture. Addition of 20 ng/ml of 25-hydroxycholecalciferol (25-OHD3) to the medium enhanced ATP content of the muscle and increased leucine incorporation into protein. Vitamin D3 at a concentration of 20 mug/ml and 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) at a concentration of 500 pg/ml were without effect. Analysis of muscle cytosol in sucrose density gradients revealed a protein fraction which specifically bound 25-OHD3 and which demonstrated a lesser affinity for 1,25-(OH)2D3. These studies suggest that 25-OHD3 may influence directly the intracellular accumulation of phosphate by muscle and thereby play an important role in the maintenance of muscle metabolism and function.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boyle I. T., Miravet L., Gray R. W., Holick M. F., Deluca H. F. The response of intestinal calcium transport to 25-hydroxy and 1,25-dihydroxy vitamin D in nephrectomized rats. Endocrinology. 1972 Mar;90(3):605–608. doi: 10.1210/endo-90-3-605. [DOI] [PubMed] [Google Scholar]
- Chen T. C., Castillo L., Korycka-Dahl M., DeLuca H. F. Role of vitamin D metabolites in phosphate transport of rat intestine. J Nutr. 1974 Aug;104(8):1056–1060. doi: 10.1093/jn/104.8.1056. [DOI] [PubMed] [Google Scholar]
- DeLuca H. F. Mechanism of action and metabolic fate of vitamin D. Vitam Horm. 1967;25:315–367. doi: 10.1016/s0083-6729(08)60039-4. [DOI] [PubMed] [Google Scholar]
- Dent C. E., Smith R. Nutritional osteomalacia. Q J Med. 1969 Apr;38(150):195–209. [PubMed] [Google Scholar]
- Fraser D. R., Kodicek E. Unique biosynthesis by kidney of a biological active vitamin D metabolite. Nature. 1970 Nov 21;228(5273):764–766. doi: 10.1038/228764a0. [DOI] [PubMed] [Google Scholar]
- Frolik C. A., DeLuca H. F. The stimulation of 1,25-dihydroxycholecalciferol metabolism in vitamin D-deficient rats by 1,25-dihydroxycholecalciferol treatment. J Clin Invest. 1973 Mar;52(3):543–548. doi: 10.1172/JCI107214. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gray R., Boyle I., DeLuca H. F. Vitamin D metabolism: the role of kidney tissue. Science. 1971 Jun 18;172(3989):1232–1234. doi: 10.1126/science.172.3989.1232. [DOI] [PubMed] [Google Scholar]
- HARRISON H. E., HARRISON H. C. Intestinal transport of phosphate: action of vitamin D, calcium, and potassium. Am J Physiol. 1961 Dec;201:1007–1012. doi: 10.1152/ajplegacy.1961.201.6.1007. [DOI] [PubMed] [Google Scholar]
- Haddad J. G., Birge S. J. Widespread, specific binding of 25-hydroxycholecalciferol in rat tissues. J Biol Chem. 1975 Jan 10;250(1):299–303. [PubMed] [Google Scholar]
- Haddad J. G., Chyu K. J. Competitive protein-binding radioassay for 25-hydroxycholecalciferol. J Clin Endocrinol Metab. 1971 Dec;33(6):992–995. doi: 10.1210/jcem-33-6-992. [DOI] [PubMed] [Google Scholar]
- Haddad J. G., Jr, Birge S. J. 25-Hydroxycholecalciferol: specific binding by rachitic tissue extracts. Biochem Biophys Res Commun. 1971 Nov;45(4):829–834. doi: 10.1016/0006-291x(71)90413-x. [DOI] [PubMed] [Google Scholar]
- Holick M. F., DeLuca H. F. A new chromatographic system for vitamin D3 and its metabolites: resoluation of a new vitamin D3 metabolite. J Lipid Res. 1971 Jul;12(4):460–465. [PubMed] [Google Scholar]
- Holmes AM ENOCH B. A., Taylor J. L., Jones M. E. Occult rickets and osteomalacia amongst the Asian immigrant population. Q J Med. 1973 Jan;42(165):125–149. [PubMed] [Google Scholar]
- KENNY A. D., MUNSON P. L. A method for the biological assay of phosphaturic activity in parathyroid extracts. Endocrinology. 1959 Apr;64(4):513–521. doi: 10.1210/endo-64-4-513. [DOI] [PubMed] [Google Scholar]
- Kowarski S., Schachter D. Effects of vitamin D on phosphate transport and incorporation into mucosal constituents of rat intestinal mucosa. J Biol Chem. 1969 Jan 10;244(1):211–217. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Marsden C. D., Reynolds E. H., Parsons V., Harris R., Duchen L. Myopathy associated with anticonvulsant osteomalacia. Br Med J. 1973 Dec 1;4(5891):526–527. doi: 10.1136/bmj.4.5891.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norman A. W. The mode of action of vitamin D. Biol Rev Camb Philos Soc. 1968 Feb;43(1):97–137. doi: 10.1111/j.1469-185x.1968.tb01111.x. [DOI] [PubMed] [Google Scholar]
- Omdahl J., Holick M., Suda T., Tanaka Y., DeLuca H. F. Biological activity of 1,25-dihydroxycholecalciferol. Biochemistry. 1971 Jul 20;10(15):2935–2940. doi: 10.1021/bi00791a022. [DOI] [PubMed] [Google Scholar]
- Ponchon G., Kennan A. L., DeLuca H. F. "Activation" of vitamin D by the liver. J Clin Invest. 1969 Nov;48(11):2032–2037. doi: 10.1172/JCI106168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raisz L. G., Trummel C. L., Holick M. F., DeLuca H. F. 1,25-dihydroxycholecalciferol: a potent stimulator of bone resorption in tissue culture. Science. 1972 Feb 18;175(4023):768–769. doi: 10.1126/science.175.4023.768. [DOI] [PubMed] [Google Scholar]
- STEENBOCK H., HERTING D. C. Vitamin D and growth. J Nutr. 1955 Dec 10;57(4):449–468. doi: 10.1093/jn/57.4.449. [DOI] [PubMed] [Google Scholar]
- Short E. M., Binder H. J., Rosenberg L. E. Familial hypophosphatemic rickets: defective transport of inorganic phosphate by intestinal mucosa. Science. 1973 Feb 16;179(4074):700–702. doi: 10.1126/science.179.4074.700. [DOI] [PubMed] [Google Scholar]
- Smith R., Stern G. Myopathy, osteomalacia and hyperparathyroidism. Brain. 1967 Sep;90(3):593–602. doi: 10.1093/brain/90.3.593. [DOI] [PubMed] [Google Scholar]
- Wasserman R. H., Taylor A. N. Intestinal absorption of phosphate in the chick: effect of vitamin D and other parameters. J Nutr. 1973 Apr;103(4):586–599. doi: 10.1093/jn/103.4.586. [DOI] [PubMed] [Google Scholar]
- ZETTNER A., SELIGSON D. APPLICATION OF ATOMIC ABSORPTION SPECTROPHOTOMETRY IN THE DETERMINATION OF CALCIUM IN SERUM. Clin Chem. 1964 Oct;10:869–890. [PubMed] [Google Scholar]
- al-Gauhari A. A., al-Nagdy S. A., el-Sabbagh M. E., Eisa E. A. Biochemical studies on some aspects of protein metabolism in vitamin-D deficient rats. Comp Biochem Physiol A Comp Physiol. 1974 Mar 1;47(3):845–854. doi: 10.1016/0300-9629(74)90459-9. [DOI] [PubMed] [Google Scholar]