Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Biochimie. 2010 Oct 26;93(2):361–368. doi: 10.1016/j.biochi.2010.10.009

Figure 4. Hypoxia induces constitutive luciferase activation in Cos7 and NIH3T3 cells.

Figure 4

(A) Cos 7 cells and (B) NIH 3T3 cells were transfected with pRL-CMV, pRL-SV40, or pRL-TK constitutive luciferase reporter using the split transfection protocol as described. Samples were treated at the oxygen concentration indicated and analyzed as described in Materials and Methods. Results are average of three independent experiments. Luciferase activity at each concentration was analyzed and is shown relative to activity at 21% oxygen. Error bars represent standard deviation. Significance was denoted as *p≤0.01 or **p≤0.001 and was determined using a one way Anova with Tukeys post hoc test.