Figure 7.

Hic-5 binds to the TBEs of the c-myc promoter in the presence of bicalutamide. The chromatin–protein complexes were pulled down by antibody for Hic-5. (a) PCR products in the input panel were amplified using diluted chromatin that was not immunoprecipitated. A mouse IgG was used to detect any nonspecific immunoprecipitated DNA. PCR products shown in gel are representatives of three separate experiments. (b) Relative changes in Hic-5 recruitment to the TBEs of c-myc promoter were quantitated by real-time qPCR, normalized by the input. Data are shown as an average of TBE1 and TBE2 binding of at least three independent experiments. ANOVA significant differences of **P<0.05.