Abstract
The paper describes the use of an extrinsic tag of inorganic radioiron to determine the total absorption of nonheme iron from a complete meal. The method was developed by measuring the iron absorbed from vegetable foods containing biosynthetically incorporated 55Fe (intrinsic tag) and from 59Fe added as a small dose of inorganic iron to the same meal (extrinsic tag). In studies with maize, black bean, and wheat, a consistent extrinsic: intrinsic radioiron absorption ratio averaging 1.10 was observed. Similar results were obtained with either ferrous or ferric iron as the extrinsic tag, and with doses of the latter ranging from 0.001 to 0.5 mg iron added to a test meal containing 2-4 mg of food iron. Adding the radioiron at different stages in preparation of the test meal also had little effect. Separate administration of the extrinsic tag was less satisfactory when small portions of a single food were employed, but with a complete meal, the separate dose was preferable. The extrinsic tag provided a valid measure of absorption despite marked differences in the iron status of the subject, and with wide changes in absorption imposed by adding desferrioxamine or ascorbic acid to the test meal. These findings indicate that there is a common pool of nonheme iron, the absorption of which is influenced by various blocking or enhancing substances present in the meal.
Full text
PDF










Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHODOS R. B., ROSS J. F., APT L., POLLYCOVE M., HALKETT J. A. The absorption of radioiron labeled foods and iron salts in normal and iron-deficient subjects and in idiopathic hemochromatosis. J Clin Invest. 1957 Feb;36(2):314–326. doi: 10.1172/JCI103427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COOK J. D., BROWN G. M., VALBERG L. S. THE EFFECT OF ACHYLIA GASTRICA ON IRON ABSORPTION. J Clin Invest. 1964 Jun;43:1185–1191. doi: 10.1172/JCI105002. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Callender S. T., Marney S. R., Jr, Warner G. T. Eggs and iron absorption. Br J Haematol. 1970 Dec;19(6):657–665. doi: 10.1111/j.1365-2141.1970.tb07010.x. [DOI] [PubMed] [Google Scholar]
- Callender S. T., Warner G. T. Iron absorption from bread. Am J Clin Nutr. 1968 Oct;21(10):1170–1174. doi: 10.1093/ajcn/21.10.1170. [DOI] [PubMed] [Google Scholar]
- DERN R. J., HART W. L. Studies with doubly labeled iron. I. Simultaneous liquid scintillation counting of isotopes Fe55 and Fe59 as ferrous perchlorate. J Lab Clin Med. 1961 Feb;57:322–330. [PubMed] [Google Scholar]
- DERN R. J., HART W. L. Studies with doubly labeled iron. II. Separation of iron from blood samples and preparation of ferrous perchlorate for liquid scintillation counting. J Lab Clin Med. 1961 Mar;57:460–467. [PubMed] [Google Scholar]
- Eakins J. D., Brown D. A. An improved method for the simultaneous determination of iron-55 and iron-59 in blood by liquid scintillation counting. Int J Appl Radiat Isot. 1966 Jul;17(7):391–397. doi: 10.1016/0020-708x(66)90065-2. [DOI] [PubMed] [Google Scholar]
- Elwood P. C., Benjamin I. T., Fry F. A., Eakins J. D., Brown D. A., De Kock P. C., Shah J. U. Absorption of iron from chapatti made from wheat flour. Am J Clin Nutr. 1970 Oct;23(10):1267–1271. doi: 10.1093/ajcn/23.10.1267. [DOI] [PubMed] [Google Scholar]
- Elwood P. C., Newton D., Eakins J. D., Brown D. A. Absorption of iron from bread. Am J Clin Nutr. 1968 Oct;21(10):1162–1169. doi: 10.1093/ajcn/21.10.1162. [DOI] [PubMed] [Google Scholar]
- GOLDBERG A., LOCHHEAD A. C., DAGG J. H. Histamine-fast achlorhydria and iron absorption. Lancet. 1963 Apr 20;1(7286):848–850. doi: 10.1016/s0140-6736(63)91623-4. [DOI] [PubMed] [Google Scholar]
- Green R., Charlton R., Seftel H., Bothwell T., Mayet F., Adams B., Finch C., Layrisse M. Body iron excretion in man: a collaborative study. Am J Med. 1968 Sep;45(3):336–353. doi: 10.1016/0002-9343(68)90069-7. [DOI] [PubMed] [Google Scholar]
- HALKETT J. A., CHODOS R. B., ROSS J. F. The labeling of human foods with radioactive iron (Fe 59). J Lab Clin Med. 1959 May;53(5):816–823. [PubMed] [Google Scholar]
- HUSSAIN R., WALKER R. B., LAYRISSE M., CLARK P., FINCH C. A. NUTRITIVE VALUE OF FOOD IRON. Am J Clin Nutr. 1965 Jun;16:464–471. doi: 10.1093/ajcn/16.6.464. [DOI] [PubMed] [Google Scholar]
- Herbert V., Gottlieb C. W., Lau K. S., Fisher M., Gevirtz N. R., Wasserman L. R. Coated charcoal assay of unsaturated iron-binding capacity. J Lab Clin Med. 1966 May;67(5):855–862. [PubMed] [Google Scholar]
- Jacobs A. M., Owen G. M. The effect of age on iron absorption. J Gerontol. 1969 Jan;24(1):95–96. doi: 10.1093/geronj/24.1.95. [DOI] [PubMed] [Google Scholar]
- Jacobs A., Owen G. M. Effect of gastric juice on iron absorption in patients with gastric atrophy. Gut. 1969 Jun;10(6):488–490. doi: 10.1136/gut.10.6.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn I. N., Monsen E. R., Cook J. D., Finch C. A. Iron absorption in man. J Lab Clin Med. 1968 May;71(5):715–721. [PubMed] [Google Scholar]
- Kuhn I. N., Roche M., Layrisse M., Martinez C., Walker R. B. Observations on the mechanism of iron absorption. Am J Clin Nutr. 1968 Oct;21(10):1184–1188. doi: 10.1093/ajcn/21.10.1184. [DOI] [PubMed] [Google Scholar]
- Layrisse M., Cook J. D., Martinez C., Roche M., Kuhn I. N., Walker R. B., Finch C. A. Food iron absorption: a comparison of vegetable and animal foods. Blood. 1969 Mar;33(3):430–443. [PubMed] [Google Scholar]
- Layrisse M., Martínez-Torres C., Roche M. Effect of interaction of various foods on iron absorption. Am J Clin Nutr. 1968 Oct;21(10):1175–1183. doi: 10.1093/ajcn/21.10.1175. [DOI] [PubMed] [Google Scholar]
- MOORE C. V., DUBACH R. Observations on the absorption of iron from foods tagged with radioiron. Trans Assoc Am Physicians. 1951;64:245–256. [PubMed] [Google Scholar]
- Martinez-Torres C., Layrisse M. Effect of amino acids on iron absorption from a staple vegetable food. Blood. 1970 May;35(5):669–682. [PubMed] [Google Scholar]
- PIRZIO-BIROLI G., BOTHWELL T. H., FINCH C. A. Iron absorption. II. The absorption of radioiron administered with a standard meal in man. J Lab Clin Med. 1958 Jan;51(1):37–48. [PubMed] [Google Scholar]
- RAMSAY W. N. The determination of the total iron-binding capacity of serum. Clin Chim Acta. 1957 Jun;2(3):221–226. doi: 10.1016/0009-8981(57)90106-7. [DOI] [PubMed] [Google Scholar]
- Retzlaff J. A., Tauxe W. N., Kiely J. M., Stroebel C. F. Erythrocyte volume, plasma volume, and lean body mass in adult men and women. Blood. 1969 May;33(5):649–661. [PubMed] [Google Scholar]
- SCHULZ J., SMITH N. J. A quantitative study of the absorption of food iron in infants and children. AMA J Dis Child. 1958 Feb;95(2):109–119. doi: 10.1001/archpedi.1958.02060050111001. [DOI] [PubMed] [Google Scholar]
- SHARPE L. M., PEACOCK W. C., COOKE R., HARRIS R. S. The effect of phytate and other food factors on iron absorption. J Nutr. 1950 Jul;41(3):433–446. doi: 10.1093/jn/41.3.433. [DOI] [PubMed] [Google Scholar]
- STEVENS A. R., Jr, PIRZIO-BIROLI G., HARKINS H. N., NYHUS L. M., FINCH C. A. Iron metabolism in patients after partial gastrectomy. Ann Surg. 1959 Apr;149(4):534–538. doi: 10.1097/00000658-195904000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]