Fig. 3.

Oligodendrocyte Cx47 cannot form functional heterotypic channels with Cx32 or Cx26 but does so readily with Cx43 or Cx30. Cells expressing Cx47 (A-E, green) were plated with cells expressing Cx32 (A, blue), Cx26 (B, blue), an empty vector control (C, blue), Cx43 (D, blue) and Cx30 (E, blue). Neurobiotin injection revealed heterotypic transfer only for Cx47-Cx43 and Cx47-Cx30 pairs. Consistent with these data, no co-localization of Cx47 with either Cx32 (F) or Cx26 (G) was evident, whereas co-localization of Cx47 with Cx43 (H) or Cx30 (I) was robust (arrowheads). Scale bar: 50 μm.