Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1973 Jan;52(1):210–214. doi: 10.1172/JCI107168

Neural Regulation of Insulin Secretion in the Dog

Daniel Porte Jr 1,2, Lucien Girardier 1,2, Josianne Seydoux 1,2, Yasunori Kanazawa 1,2, Jean Posternak 1,2
PMCID: PMC302245  PMID: 4682384

Abstract

The effects of stimulation of the mixed autonomic nerve to the dog pancreas has been studied under conditions in which both pancreaticoduodenal vein blood flow and insulin concentration were determined. Stimulation resulted in increased insulin output, which was blocked by prior administration of atropine. Blood flow was reduced by stimulation in proportion to the rate of stimulation. At 40 stimuli/s a maximum effect was found at 1 min with a gradual return toward base line despite continued application of the stimulus. Atropinization had no effect on blood flow changes. Insulin responses to 0.1 g/kg glucose were reduced on the average 40% by simultaneous stimulation of the pancreatic nerve at 40 cycles/s in atropinized animals. These studies establish this preparation as a reproducible model for the direct examination of autonomic influences on endocrine pancreatic function. From them it is concluded that the nerve supply to the endocrine pancreas of the dog is sufficient to inhibit insulin secretion by activation of the sympathetic nerves and to stimulate insulin secretion by activation of the parasympathetic nerves.

Full text

PDF
210

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison S. P., Hinton P., Chamberlain M. J. Intravenous glucose-tolerance, insulin, and free-fatty-acid levels in burned patients. Lancet. 1968 Nov 23;2(7578):1113–1116. doi: 10.1016/s0140-6736(68)91581-x. [DOI] [PubMed] [Google Scholar]
  2. Dupré J. Regulation of the secretions of the pancreas. Annu Rev Med. 1970;21:299–316. doi: 10.1146/annurev.me.21.020170.001503. [DOI] [PubMed] [Google Scholar]
  3. Esterhuizen A. C., Spriggs T. L., Lever J. D. Nature of islet-cell innervation in the cat pancreas. Diabetes. 1968 Jan;17(1):33–36. doi: 10.2337/diab.17.1.33. [DOI] [PubMed] [Google Scholar]
  4. Frohman L. A., Ezdinli E. Z., Javid R. Effect of vagotomy and vagal stimulation on insulin secretion. Diabetes. 1967 Jul;16(7):443–448. doi: 10.2337/diab.16.7.443. [DOI] [PubMed] [Google Scholar]
  5. Kajinuma H., Kaneto A., Kuzuya T., Nakao K. Effects of methacholine on insulin secretion in man. J Clin Endocrinol Metab. 1968 Sep;28(9):1384–1388. doi: 10.1210/jcem-28-9-1384. [DOI] [PubMed] [Google Scholar]
  6. Kanazawa Y., Kuzuya T., Ide T. Insulin output via the pancreatic vein and plasma insulin response to glucose in dogs. Am J Physiol. 1968 Sep;215(3):620–626. doi: 10.1152/ajplegacy.1968.215.3.620. [DOI] [PubMed] [Google Scholar]
  7. Kaneto A., Kosaka K., Nakao K. Effects of stimulation of the vagus nerve on insulin secretion. Endocrinology. 1967 Mar;80(3):530–536. doi: 10.1210/endo-80-3-530. [DOI] [PubMed] [Google Scholar]
  8. Lerner R. L., Porte D., Jr Epinephrine: selective inhibition of the acute insulin response to glucose. J Clin Invest. 1971 Nov;50(11):2453–2457. doi: 10.1172/JCI106744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Malaisse W., Malaisse-Lagae F., Wright P. H., Ashmore J. Effects of adrenergic and cholinergic agents upon insulin secretion in vitro. Endocrinology. 1967 May;80(5):975–978. doi: 10.1210/endo-80-5-975. [DOI] [PubMed] [Google Scholar]
  10. Orci L., Lambert A. E., Amherdt M., Cameron D., Kanazawa Y., Stauffacher W. [The autonomous nervous system and the B-cell: metabolic and morphological observations made in spiny mice (Acomys cahirinus) and in cultured fetal rat pancreas]. Acta Diabetol Lat. 1970 Sep;7 (Suppl 1):184–228. [PubMed] [Google Scholar]
  11. Porte D., Jr A receptor mechanism for the inhibition of insulin release by epinephrine in man. J Clin Invest. 1967 Jan;46(1):86–94. doi: 10.1172/JCI105514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Porte D., Jr Sympathetic regulation of insulin secretion. Its relation to diabetes mellitus. Arch Intern Med. 1969 Mar;123(3):252–260. [PubMed] [Google Scholar]
  13. Rappaport A. M., Kawamura T., Davidson J. K., Lin B. J., Oira S., Zeigler M., Coddling J. A., Henderson J., Haist R. E. Effects of hormones and of blood flow on insulin output of isolated pancreas in situ. Am J Physiol. 1971 Jul;221(1):343–348. doi: 10.1152/ajplegacy.1971.221.1.343. [DOI] [PubMed] [Google Scholar]
  14. Taylor S. H., Majid P. A. Insulin and the heart. J Mol Cell Cardiol. 1971 Aug;2(3):293–317. doi: 10.1016/0022-2828(71)90060-5. [DOI] [PubMed] [Google Scholar]
  15. Watari N. Fine structure of nervous elements in the pancreas of some vertebrates. Z Zellforsch Mikrosk Anat. 1968;85(3):291–314. doi: 10.1007/BF00328843. [DOI] [PubMed] [Google Scholar]
  16. Woods S. C., Alexander K. R., Porte D., Jr Conditioned insulin secretion and hypoglycemia following repeated injections of tolbutamide in rats. Endocrinology. 1972 Jan;90(1):227–231. doi: 10.1210/endo-90-1-227. [DOI] [PubMed] [Google Scholar]
  17. Woods S. C., Hutton R. A., Makous W. Conditioned insulin secretion in the albino rat. Proc Soc Exp Biol Med. 1970 Mar;133(3):964–968. doi: 10.3181/00379727-133-34605. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES