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. 1973 Feb;52(2):236–247. doi: 10.1172/JCI107180

The Interaction of Serum and Arterial Lipoproteins with Elastin of the Arterial Intima and Its Role in the Lipid Accumulation in Atherosclerotic Plaques

Dieter M Kramsch 1, William Hollander 1
PMCID: PMC302253  PMID: 4346005

Abstract

Arterial elastin appears to be a proteinlipid complex with the lipid component being bound to elastin peptide groups. In atherosclerotic lesions the lipid content of elastin increases progressively with increasing severity of atherosclerosis. The increases in the lipid content of plaque elastin are mainly due to large increases in cholesterol with about 80% of the cholesterol being cholesterol ester. This deposition of cholesterol in elastin accounts for a substantial part of the total cholesterol accumulation in atherosclerotic lesions of all stages. The present in vitro study suggests that the mechanism involved in the deposition of lipids in arterial elastin may be an interaction of the elastin protein with serum or arterial low density or very low density lipoproteins (LDL and VLDL) resulting in a transfer of lipids, but not of lipoprotein protein to the elastin. No significant lipid transfer occurred from the high density lipoproteins or chylomicrons. The amount of lipid taken up by plaque elastin was strikingly higher than by normal elastin and consisted mainly of cholesterol with over 80% of the cholesterol being cholesterol ester. The precondition for the lipid accumulation in plaque elastin appeared to be an altered amino acid composition of the elastin protein consisting of an increase in polar amino acids and a reduction in cross-linking amino acids. Subsequent treatment of lipoprotein-incubated arterial elastin with hot alkali and apolipoproteins did not reverse the binding of lipoprotein lipid to diseased elastin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS C. W., TUQAN N. A. Elastic degeneration as source of lipids in the early lesion of atherosclerosis. J Pathol Bacteriol. 1961 Jul;82:131–139. doi: 10.1002/path.1700820116. [DOI] [PubMed] [Google Scholar]
  2. AVIGAN J. Modification of human serum lipoprotein fractions by lipide extraction. J Biol Chem. 1957 Jun;226(2):957–964. [PubMed] [Google Scholar]
  3. FRIEDMAN M. PATHOGENESIS OF THE SPONTANEOUS ATHEROSCLEROTIC PLAQUE. A STUDY ON THE A/FRIEDMAN M: PATHOGENESIS OF THE SPONTANEOUS ATHEROSCLEROTIC PLAQUE. A STUDY ON THE CHOLESTEROL-FED RABBIT. Arch Pathol. 1963 Sep;76:318–329. [PubMed] [Google Scholar]
  4. Fredrickson D. S., Levy R. I., Lees R. S. Fat transport in lipoproteins--an integrated approach to mechanisms and disorders. N Engl J Med. 1967 Jan 19;276(3):148–contd. doi: 10.1056/NEJM196701192760305. [DOI] [PubMed] [Google Scholar]
  5. Fredrickson D. S., Levy R. I., Lees R. S. Fat transport in lipoproteins--an integrated approach to mechanisms and disorders. N Engl J Med. 1967 Jan 26;276(4):215–contd. doi: 10.1056/NEJM196701262760406. [DOI] [PubMed] [Google Scholar]
  6. Fredrickson D. S., Levy R. I., Lees R. S. Fat transport in lipoproteins--an integrated approach to mechanisms and disorders. N Engl J Med. 1967 Feb 2;276(5):273–concl. doi: 10.1056/NEJM196702022760507. [DOI] [PubMed] [Google Scholar]
  7. GITLIN D., CORNWELL D. G., NAKASATO D., ONCLEY J. L., HUGHES W. L., Jr, JANEWAY C. A. Studies on the metabolism of plasma proteins in the nephrotic syndrome. II. The lipoproteins. J Clin Invest. 1958 Feb;37(2):172–184. doi: 10.1172/JCI103596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollander W., Kramsch D. M. The distribution of intravenously administered [3H] cholesterol in the arteries and other tissues. 1. Tissue fractionation findings. J Atheroscler Res. 1967 Jul-Aug;7(4):491–500. doi: 10.1016/s0368-1319(67)80026-7. [DOI] [PubMed] [Google Scholar]
  10. Kramsch D. M., Franzblau C., Hollander W. The protein and lipid composition of arterial elastin and its relationship to lipid accumulation in the atherosclerotic plaque. J Clin Invest. 1971 Aug;50(8):1666–1677. doi: 10.1172/JCI106656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kramsch D. M., Gore I., Hollander W. The distribution of intravenously administered [3H] cholesterol in the arteries and other tissues. 2. Radioautographic findings. J Atheroscler Res. 1967 Jul-Aug;7(4):501–516. doi: 10.1016/s0368-1319(67)80027-9. [DOI] [PubMed] [Google Scholar]
  12. Kramsch D. M., Hollander W. Occlusive atheroslcerotic disease of the coronary arteries in monkey (Macaca irus) induced by diet. Exp Mol Pathol. 1968 Aug;9(1):1–22. doi: 10.1016/0014-4800(68)90045-2. [DOI] [PubMed] [Google Scholar]
  13. LANSING A. I., ALEX M., ROSENTHAL T. B. Atheromatosis as a sequel to senescent changes in the arterial wall. J Gerontol. 1950 Oct;5(1-4):314–318. doi: 10.1093/geronj/5.4.314. [DOI] [PubMed] [Google Scholar]
  14. LANSING A. I., ROSENTHAL T. B., ALEX M., DEMPSEY E. W. The structure and chemical characterization of elastic fibers as revealed by elastase and by electron microscopy. Anat Rec. 1952 Dec;114(4):555–575. doi: 10.1002/ar.1091140404. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MOON H. D., RINEHART J. F. Histogenesis of coronary arteriosclerosis. Circulation. 1952 Oct;6(4):481–488. doi: 10.1161/01.cir.6.4.481. [DOI] [PubMed] [Google Scholar]
  17. Robert A. M., Grosgogeat Y., Reverdy V., Robert B., Robert L. Lésions artérielles produites chez le lapin par immunisation avec l'élastine et les glycoprotéines de structure de l'aorte. Atherosclerosis. 1971 May-Jun;13(3):427–449. doi: 10.1016/0021-9150(71)90084-0. [DOI] [PubMed] [Google Scholar]
  18. Ross R., Bornstein P. The elastic fiber. I. The separation and partial characterization of its macromolecular components. J Cell Biol. 1969 Feb;40(2):366–381. doi: 10.1083/jcb.40.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scanu A. Forms of human serum high density lipoprotein protein. J Lipid Res. 1966 Mar;7(2):295–306. [PubMed] [Google Scholar]
  20. Smith E. B., Evans P. H., Downham M. D. Lipid in the aortic intima. The correlation of morphological and chemical characteristics. J Atheroscler Res. 1967 Mar-Apr;7(2):171–186. doi: 10.1016/s0368-1319(67)80079-6. [DOI] [PubMed] [Google Scholar]
  21. VAN HANDEL E., ZILVERSMIT D. B. Micromethod for the direct determination of serum triglycerides. J Lab Clin Med. 1957 Jul;50(1):152–157. [PubMed] [Google Scholar]
  22. Yu S. Y. Cross-linking of elastin in human atherosclerotic aortas. I. A preliminary study. Lab Invest. 1971 Aug;25(2):121–125. [PubMed] [Google Scholar]
  23. ZUGIBE F. T., BROWN K. D. Histochemical studies in atherogenesis: human aortas. Circ Res. 1960 Jan;8:287–295. doi: 10.1161/01.res.8.1.287. [DOI] [PubMed] [Google Scholar]

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