Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1973 Feb;52(2):483–492. doi: 10.1172/JCI107205

Altered Lipid Metabolism in Human Platelets after Primary Aggregation

Daniel Deykin 1
PMCID: PMC302278  PMID: 4683885

Abstract

Thrombin and poly-l-lysine alter the incorporation of acetate, glycerol, and fatty acids into the lipids of washed human platelets. Both aggregating agents decrease the incorporation of acetate into all lipid classes other than free fatty acids. Similarly, glycerol incorporation into complex lipids is impaired by both thrombin and polylysine. Thrombin caused marked depression of the incorporation of palmitic acid into both lecithin and triglycerides. By contrast it enhanced the incorporation of oleic acid into lecithin, but not into triglycerides. The data suggest that the process of primary platelet aggregation is associated with a defect in the assembly of complex lipids.

Full text

PDF
483

Images in this article

489Image
on p.489

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baenziger N. L., Brodie G. N., Majerus P. W. A thrombin-sensitive protein of human platelet membranes. Proc Natl Acad Sci U S A. 1971 Jan;68(1):240–243. doi: 10.1073/pnas.68.1.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baenziger N. L., Brodie G. N., Majerus P. W. Isolation and properties of a thrombin-sensitive protein of human platelets. J Biol Chem. 1972 May 10;247(9):2723–2731. [PubMed] [Google Scholar]
  3. Cohen P., Derksen A., Van den Bosch H. Pathways of fatty acid metabolism in human platelets. J Clin Invest. 1970 Jan;49(1):128–139. doi: 10.1172/JCI106211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corn M. Effect of thrombin on glycolysis of fresh and stored platelets. J Appl Physiol. 1966 Jan;21(1):62–66. doi: 10.1152/jappl.1966.21.1.62. [DOI] [PubMed] [Google Scholar]
  5. Deykin D., Cochios F., DeCamp G., Lopez A. Hepatic removal of activated factor X by the perfused rabbit liver. Am J Physiol. 1968 Feb;214(2):414–419. doi: 10.1152/ajplegacy.1968.214.2.414. [DOI] [PubMed] [Google Scholar]
  6. Deykin D., Desser R. K. The incorporation of acetate and palmitate into lipids by human platelets. J Clin Invest. 1968 Jul;47(7):1590–1602. doi: 10.1172/JCI105851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elsbach P., Pettis P., Marcus A. Lysolecithin metabolism by human platelets. Blood. 1971 Jun;37(6):675–683. [PubMed] [Google Scholar]
  8. Hennes A. R., Awai K., Hammarstrand K., Duboff G. S. Carbon-14 in carboxyl carbon of fatty acids formed by platelets from normal and diabetic subjects. Nature. 1966 May 21;210(5038):839–841. doi: 10.1038/210839a0. [DOI] [PubMed] [Google Scholar]
  9. Hoak J. C., Spector A. A., Fry G. L., Barnes B. C. Localization of free fatty acids taken up by human platelets. Blood. 1972 Jul;40(1):16–22. [PubMed] [Google Scholar]
  10. Jenkins C. S., Packham M. A., Kinlough-Rathbone R. L., Mustard J. F. Interactions of polylysine with platelets. Blood. 1971 Apr;37(4):395–412. [PubMed] [Google Scholar]
  11. Karpatkin S. Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate, and agglutination and contraction on platelet glycolysis. J Clin Invest. 1967 Mar;46(3):409–417. doi: 10.1172/JCI105542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lewis N., Majerus P. W. Lipid metabolism in human platelets. II. De novo phospholipid synthesis and the effect of thrombin on the pattern of synthesis. J Clin Invest. 1969 Nov;48(11):2114–2123. doi: 10.1172/JCI106178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Majerus P. W., Brodie G. N. The binding of phytohemagglutinins to human platelet plasma membranes. J Biol Chem. 1972 Jul 10;247(13):4253–4257. [PubMed] [Google Scholar]
  14. Majerus P. W., Smith M. B., Clamon G. H. Lipid metabolism in human platelets. I. Evidence for a complete fatty acid synthesizing system. J Clin Invest. 1969 Jan;48(1):156–164. doi: 10.1172/JCI105964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Okuma M., Steiner M., Baldini M. G. Incorporation of acetate and fatty acids into lipids of rat platelets. Proc Soc Exp Biol Med. 1971 Mar;136(3):842–847. doi: 10.3181/00379727-136-35377. [DOI] [PubMed] [Google Scholar]
  16. Okuma M., Steiner M., Baldini M. Lipid content and in vitro incorporation of free fatty acids into lipids of human platelets: the effect of storage at 4 degrees C. Blood. 1971 Jul;38(1):27–38. [PubMed] [Google Scholar]
  17. Spector A. A., Hoak J. C., Warner E. D., Fry G. L. Utilization of long-chain free fatty acids by human platelets. J Clin Invest. 1970 Aug;49(8):1489–1496. doi: 10.1172/JCI106366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Warshaw A. L., Laster L., Shulman N. R. The stimulation by thrombin of glucose oxidation in human platelets. J Clin Invest. 1966 Dec;45(12):1923–1934. doi: 10.1172/JCI105497. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES