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. 1973 Feb;52(2):524–527. doi: 10.1172/JCI107211

Activation of Renal Cortical Adenylate Cyclase by Circulating Immunoreactive Parathyroid Hormone Fragments

Janet M Canterbury 1,2, Gerald S Levey 1,2, Eric Reiss 1,2
PMCID: PMC302284  PMID: 4346008

Abstract

Three distinct immunoreactive species of parathyroid hormone (PTH) are present in human serum. One has an estimated mol wt of 9,500 and probably represents glandular hormone, the second 7,000-7,500 mol wt, and the third 4,500-5,000 mol wt. In order to assess the biological activity of these circulating forms of PTH, we determined their ability to activate renal cortical adenylate cyclase. The 9,500 mol wt and 4,500-5,000 mol wt fractions produced four- to sixfold increases in cyclic 3′,5′-AMP accumulation above control; the 7,000-7,500 mol wt fraction was inactive. None of the fragments had any effects on phosphodiesterase activity. Antiserum to bovine PTH did not block the activation of adenylate cyclase by either the gragments or bovine PTH. The data suggest that a large proportion of circulating immunoreactive human PTH is biologically active and that the biologically and immunologically active sites of the hormone are distinct.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnaud C. D., Tsao H. S., Littledike T. Radioimmunoassay of human parathyroid hormone in serum. J Clin Invest. 1971 Jan;50(1):21–34. doi: 10.1172/JCI106476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Canterbury J. M., Reiss E. Multiple immunoreactive molecular forms of parathyroid hormone in human serum. 1. Proc Soc Exp Biol Med. 1972 Sep;140(4):1393–1398. doi: 10.3181/00379727-140-36681. [DOI] [PubMed] [Google Scholar]
  3. Chase L. R., Aurbach G. D. Parathyroid function and the renal excretion of 3'5'-adenylic acid. Proc Natl Acad Sci U S A. 1967 Aug;58(2):518–525. doi: 10.1073/pnas.58.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chase L. R., Aurbach G. D. Renal adenyl cyclase: anatomically separate sites for parathyroid hormone and vasopressin. Science. 1968 Feb 2;159(3814):545–547. doi: 10.1126/science.159.3814.545. [DOI] [PubMed] [Google Scholar]
  5. Fischer J. A., Oldham S. B., Sizemore G. W., Arnaud C. D. Calcium-regulated parathyroid hormone peptidase. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2341–2345. doi: 10.1073/pnas.69.8.2341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Habener J. F., Powell D., Murray T. M., Mayer G. P., Potts J. T., Jr Parathyroid hormone: secretion and metabolism in vivo. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2986–2991. doi: 10.1073/pnas.68.12.2986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Habener J. F., Segre G. V., Powell D., Murray T. M., Potts J. T., Jr Immunoreactive parathyroid hormone in circulation of man. Nat New Biol. 1972 Aug 2;238(83):152–154. doi: 10.1038/newbio238152a0. [DOI] [PubMed] [Google Scholar]
  8. Krishna G., Weiss B., Brodie B. B. A simple, sensitive method for the assay of adenyl cyclase. J Pharmacol Exp Ther. 1968 Oct;163(2):379–385. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Levey G. S., Epstein S. E. Myocardial adenyl cyclase: activation by thyroid hormones and evidence for two adenyl cyclase systems. J Clin Invest. 1969 Sep;48(9):1663–1669. doi: 10.1172/JCI106131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reis E., Canterbury J. M. A radioimmunoassay for parathyroid hormone in man. Proc Soc Exp Biol Med. 1968 Jun;128(2):501–504. doi: 10.3181/00379727-128-33050. [DOI] [PubMed] [Google Scholar]
  12. Roof B. S., Carpenter B., Fink D. J., Gordan G. S. Some thoughts on the nature of ectopic parathyroid hormones. Am J Med. 1971 May;50(5):686–691. doi: 10.1016/0002-9343(71)90124-0. [DOI] [PubMed] [Google Scholar]
  13. Sherwood L. M., Mayer G. P., Ramberg C. F., Jr, Kronfeld D. S., Aurbach G. D., Potts J. T., Jr Regulation of parathyroid hormone secretion: proportional control by calcium, lack of effect of phosphate. Endocrinology. 1968 Nov;83(5):1043–1051. doi: 10.1210/endo-83-5-1043. [DOI] [PubMed] [Google Scholar]

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