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. 1973 Mar;52(3):612–623. doi: 10.1172/JCI107223

Sodium Chloride and Water Transport in the Medullary Thick Ascending Limb of Henle. EVIDENCE FOR ACTIVE CHLORIDE TRANSPORT

Antonino S Rocha 1, Juha P Kokko 1
PMCID: PMC302300  PMID: 4685086

Abstract

Transport of NaCl and water was examined in the rabbit medullary thick ascending limb of Henle (ALH) by perfusing isolated segments of these nephrons in vitro. Osmotic water permeability was evaluated by perfusing tubules against imposed osmotic gradients. In these experiments the net transport of fluid remained at zero when segments of thick ALH were perfused with isotonic ultrafiltrate in a bath of rabbit serum in which the serum osmolality was increased by the addition of either 239±8 mosmol/liter of raffinose or 232±17 mosmol of NaCl indicating that the thick ascending limb of Henle is impermeant to osmotic flow of water. When these tubules were perfused at slow rates with isosmolal ultrafiltrate of same rabbit serum as used for the bath, the effluent osmolality was consistently lowered to concentrations less than the perfusate and the bath. That this decrease in collected fluid osmolality represented salt transport was demonstrated in a separate set of experiments in which it was shown that the sodium and chloride concentrations decreased to 0.79±0.02 and 0.77±0.02 respectively when compared with the perfusion fluid concentrations. In each instance the simultaneously determined transtubular potential difference (PD) revealed the lumen to be positive with the magnitude dependent on the perfusion rate. At flow rates above 2 nl·min-1, the mean transtubular PD was stable and equal to 6.70±0.34 mv. At stop-flow conditions this PD became more positive. Ouabain and cooling reversibly decreased the magnitude of this PD. The transtubular PD remained positive, 3.3±0.2 mV, when complete substitution of Na by choline was carried out in both the perfusion fluid and the bathing media. These results are interpreted to indicate that the active transport process is primarily an electrogenic chloride mechanism. The isotopic permeability coefficient for Na was 6.27±0.38 × 10-5 cm·s-1 indicating that the thick ALH is approximately as permeable to Na as the proximal convoluted tubule. The chloride permeability coefficient for the thick ALH was 1.06±0.12 × 10-5 cm·s-1 which is significantly less than the chloride permeability of the proximal tubule.

These data demonstrate that the medullary thick ascending limb of Henle is water impermeable while having the capacity for active outward solute transport as a consequence of an electrogenic chloride pump. The combination of these characteristics allows this segment to generate a dilute tubular fluid and participate as the principal energy source for the overall operation of the countercurrent multiplication system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett C. M., Clapp J. R., Berliner R. W. Micropuncture study of the proximal and distal tubule in the dog. Am J Physiol. 1967 Nov;213(5):1254–1262. doi: 10.1152/ajplegacy.1967.213.5.1254. [DOI] [PubMed] [Google Scholar]
  2. Boulpaep E. L., Seely J. F. Electrophysiology of proximal and distal tubules in the autoperfused dog kidney. Am J Physiol. 1971 Oct;221(4):1084–1096. doi: 10.1152/ajplegacy.1971.221.4.1084. [DOI] [PubMed] [Google Scholar]
  3. Brunton W. J., Brinster R. L. Active chloride transport in the isolated rabbit oviduct. Am J Physiol. 1971 Aug;221(2):658–661. doi: 10.1152/ajplegacy.1971.221.2.658. [DOI] [PubMed] [Google Scholar]
  4. Burg M. B., Issaacson L., Grantham J., Orloff J. Electrical properties of isolated perfused rabbit renal tubules. Am J Physiol. 1968 Oct;215(4):788–794. doi: 10.1152/ajplegacy.1968.215.4.788. [DOI] [PubMed] [Google Scholar]
  5. Burg M. B., Orloff J. Electrical potential difference across proximal convoluted tubules. Am J Physiol. 1970 Dec;219(6):1714–1716. doi: 10.1152/ajplegacy.1970.219.6.1714. [DOI] [PubMed] [Google Scholar]
  6. Clapp J. R., Robinson R. R. Osmolality of distal tubular fluid in the dog. J Clin Invest. 1966 Dec;45(12):1847–1853. doi: 10.1172/JCI105488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finn A. L., Handler J. S., Orloff J. Active chloride transport in the isolated toad bladder. Am J Physiol. 1967 Jul;213(1):179–184. doi: 10.1152/ajplegacy.1967.213.1.179. [DOI] [PubMed] [Google Scholar]
  8. GLYNN I. M. THE ACTION OF CARDIAC GLYCOSIDES ON ION MOVEMENTS. Pharmacol Rev. 1964 Dec;16:381–407. [PubMed] [Google Scholar]
  9. GOTTSCHALK C. W., LASSITER W. E., MYLLE M., ULLRICH KJ SCHMIDT-NIELSEN B., O'DELL R., PEHLING G. Micropuncture study of composition of loop of Henle fluid in desert rodents. Am J Physiol. 1963 Apr;204:532–535. doi: 10.1152/ajplegacy.1963.204.4.532. [DOI] [PubMed] [Google Scholar]
  10. Grantham J. J., Kurg M. B., Obloff J. The nature of transtubular Na and K transport in isolated rabbit renal collecting tubules. J Clin Invest. 1970 Oct;49(10):1815–1826. doi: 10.1172/JCI106399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imai M., Kokko J. P. Effect of peritubular protein concentration on reabsorption of sodium and water in isolated perfused proxmal tubules. J Clin Invest. 1972 Feb;51(2):314–325. doi: 10.1172/JCI106816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JARAUSCH K. H., ULLRICH K. J. Untersuchungen zum Problem der Harnkonzentrierung und Harnverdünnung; Uber die Verteilung von Elektrolyten (Na, K, Ca, Mg, Cl, anorganischem Phosphat), Harnstoff, Aminosäuren und exogenem Kreatinin in Rinde und Mark der Hundeniere bei verschiedenen Diuresezuständen. Pflugers Arch. 1956;262(6):537–550. doi: 10.1007/BF00362116. [DOI] [PubMed] [Google Scholar]
  13. Kokko J. P., Rector F. C. Flow dependence of transtubular potential difference in isolated perfused segments of rabbit proximal convoluted tubule. J Clin Invest. 1971 Dec;50(12):2745–2750. doi: 10.1172/JCI106776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kokko J. P. Sodium chloride and water transport in the descending limb of Henle. J Clin Invest. 1970 Oct;49(10):1838–1846. doi: 10.1172/JCI106401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kokko J. P. Urea transport in proximal tubule and the descending limb of Henle. J Clin Invest. 1972 Aug;51(8):1999–2008. doi: 10.1172/JCI107006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LEVITIN H., GOODMAN A., PIGEON G., EPSTEIN F. H. Composition of the renal medulla during water diuresis. J Clin Invest. 1962 May;41:1145–1151. doi: 10.1172/JCI104567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MACROBBIE E. A., USSING H. H. Osmotic behaviour of the epithelial cells of frog skin. Acta Physiol Scand. 1961 Nov-Dec;53:348–365. doi: 10.1111/j.1748-1716.1961.tb02293.x. [DOI] [PubMed] [Google Scholar]
  18. Malnic G., Klose R. M., Giebisch G. Micropuncture study of distal tubular potassium and sodium transport in rat nephron. Am J Physiol. 1966 Sep;211(3):529–547. doi: 10.1152/ajplegacy.1966.211.3.529. [DOI] [PubMed] [Google Scholar]
  19. RECTOR F. C., Jr, CLAPP J. R. Evidence for active chloride reabsorption in the distal renal tubule of the rat. J Clin Invest. 1962 Jan;41:101–107. doi: 10.1172/JCI104451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosin J. M., Katz M. A., Rector F. C., Jr, Seldin D. W. Acetazolamide in studying sodium reabsorption in diluting segment. Am J Physiol. 1970 Dec;219(6):1731–1738. doi: 10.1152/ajplegacy.1970.219.6.1731. [DOI] [PubMed] [Google Scholar]
  21. Schmidt U., Dubach U. C. Activity of (Na+K+)-stimulated adenosintriphosphatase in the rat nephron. Pflugers Arch. 1969;306(3):219–226. doi: 10.1007/BF00592433. [DOI] [PubMed] [Google Scholar]
  22. Schnermann J. Microperfusion study of single short loops of Henle in rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1968;300(4):255–282. doi: 10.1007/BF00364298. [DOI] [PubMed] [Google Scholar]
  23. ULLRICH K. J., SCHMIDT-NIELSON B., O'DELL R., PEHLING G., GOTTSCHALK C. W., LASSITER W. E., MYLLE M. Micropuncture study of composition of proximal and distal tubular fluid in rat kidney. Am J Physiol. 1963 Apr;204:527–531. doi: 10.1152/ajplegacy.1963.204.4.527. [DOI] [PubMed] [Google Scholar]
  24. Valtin H. Sequestration of urea and nonurea solutes in renal tissues of rats with hereditary hypothalamic diabetes insipidus: effect of vasopressin and dehydration on the countercurrent mechanism. J Clin Invest. 1966 Mar;45(3):337–345. doi: 10.1172/JCI105348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WINDHAGER E. E., GIEBISCH G. Micropuncture study of renal tubular transfer of sodium chloride in the rat. Am J Physiol. 1961 Mar;200:581–590. doi: 10.1152/ajplegacy.1961.200.3.581. [DOI] [PubMed] [Google Scholar]
  26. Zadunaisky J. A. Active transport of chloride in frog cornea. Am J Physiol. 1966 Aug;211(2):506–512. doi: 10.1152/ajplegacy.1966.211.2.506. [DOI] [PubMed] [Google Scholar]

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