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. 1973 May;52(5):1041–1051. doi: 10.1172/JCI107269

Dynamics of Insulin Release and Microtubular-Microfilamentous System. I. EFFECT OF CYTOCHALASIN B

E Van Obberghen 1,2, G Somers 1,2, G Devis 1,2, G D Vaughan 1,2, F Malaisse-Lagae 1,2, L Orci 1,2, W J Malaisse 1,2
PMCID: PMC302358  PMID: 4573352

Abstract

In order to assess the participation of the microfilamentous cell web in the multiphasic response of the pancreatic beta cell, the effect of cytochalasin B upon both glucose- and sulfonylurea-induced insulin release was investigated in the perfused isolated pancreas. Cytochalasin B failed to affect the basal rate of insulin release, but enhanced the initial and later phases of insulin secretion in response to either glucose or gliclazide. In addition, cytochalasin B lowered the threshold concentration for the stimulant action of glucose upon insulin release. Ultrastructural studies supported the concept of a specific interaction of cytochalasin B with the microfilamentous cell web of the beta cell. It is concluded that the integrity of such a structure is equally important for both the initial and later secretory responses of the beta cell to various insulinotropic agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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