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. 1973 Aug;52(8):1841–1844. doi: 10.1172/JCI107366

Neural Control of Counter-Regulatory Events during Glucopenia in Man

Robert G Brodows 1,2, F Xavier Pi-Sunyer 1,2, Robert G Campbell 1,2
PMCID: PMC302464  PMID: 4719665

Abstract

The effect of autonomic denervation on the metabolic and hormonal responses during intracellular glucopenia in man was investigated. 2-Deoxy-d-glucose (2 DG), a competitive inhibitor of glucose metabolism, was administered intravenously to nine normal volunteers and to five patients, three with complete cervical cord transection (C-6) and two with idiopathic orthostatic hypotension. Before, during, and after a 20 min infusion of 2 DG (50 mg/kg) plasma concentrations of glucose, lactate, FFA, total catecholamines, immunoreactive insulin (IRI), human growth hormone (HGH), and cortisol were determined for periods up to 150 min. In control subjects, the initial elevation of FFA, glucose. HGH, and cortisol corresponded with the rise in total catecholamines, with maximal levels attained at 60 min, lactate rose at a slower rate, reaching peak levels at 150 min: although no change in IRI was noted. In contrast, 2 DG-induced glucopenic stress in the autonomic denervated subjects was characterized by no detectable catecholamine release or significant rise in glucose, FFA, lactate, or IRI. However, HGH and cortisol responses in four of the five patients were of a similar or greater magnitude than controls.

These studies demonstrate that the integrity of the sympathoadrenomedullary axis is essential for the counter-regulatory response to intracellular glucopenia in man. Cortisol and HGH have no apparent role in these events.

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Selected References

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  1. ALTSZULER N., DUNN A., STEELE R., BISHOP J. S., DE BODO R. C. Effect of 2-deoxyglucose on glucose turnover in normal and adrenalectomized dogs. Am J Physiol. 1963 Jun;204:1008–1012. doi: 10.1152/ajplegacy.1963.204.6.1008. [DOI] [PubMed] [Google Scholar]
  2. DISALVO R. J., BLOOM W. L., BRUST A. A., FERGUSON R. W., FERRIS E. B. A comparison of the metabolic and circulatory effects of epinephrine, nor-epinephrine and insulin hypoglycemia with observations on the influence of autonomic blocking agents. J Clin Invest. 1956 May;35(5):568–577. doi: 10.1172/JCI103310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Frohman L. A., Bernardis L. L. Effect of hypothalamic stimulation on plasma glucose, insulin, and glucagon levels. Am J Physiol. 1971 Dec;221(6):1596–1603. doi: 10.1152/ajplegacy.1971.221.6.1596. [DOI] [PubMed] [Google Scholar]
  4. Goldfien A., Gullixson K. S., Hargrove G. Evidence for centers in the central nervous system that regulate fat mobilization in dogs. J Lipid Res. 1966 May;7(3):357–367. [PubMed] [Google Scholar]
  5. Goldsmith S. J., Yalow R. S., Berson S. A. Effects of 2-deoxy-D-glucose on insulin-secretory responses to intravenous glucose, glucagon, tolbutamide and arginine in man. Diabetes. 1970 Jun;19(6):453–457. doi: 10.2337/diab.19.6.453. [DOI] [PubMed] [Google Scholar]
  6. Grasso S. G., Karam J. H., Wegienka L. C., Grodsky G. M., Forsham P. H. Use of 2-deoxy-D-glucose in studying the mechanism of lipid mobilization. J Clin Endocrinol Metab. 1968 Apr;28(4):535–542. doi: 10.1210/jcem-28-4-535. [DOI] [PubMed] [Google Scholar]
  7. HOCHELLA N. J., WEINHOUSE S. AUTOMATED LACTIC ACID DETERMINATION IN SERUM AND TISSUE EXTRACTS. Anal Biochem. 1965 Feb;10:304–317. doi: 10.1016/0003-2697(65)90271-x. [DOI] [PubMed] [Google Scholar]
  8. HOKFELT B., BYDGEMAN S. Increased adrenaline production following administration of 2-deoxy-D-glucose in the rat. Proc Soc Exp Biol Med. 1961 Mar;106:537–539. doi: 10.3181/00379727-106-26394. [DOI] [PubMed] [Google Scholar]
  9. Kokka N., Eisenberg R. M., Garcia J., George R. Blood glucose, growth hormone, and cortisol levels after hypothalamic stimulation. Am J Physiol. 1972 Feb;222(2):296–301. doi: 10.1152/ajplegacy.1972.222.2.296. [DOI] [PubMed] [Google Scholar]
  10. LUFT R., VON EULER U. S. Two cases of postural hypotension showing a deficiency in release of nor-epinephrine and epinephrine. J Clin Invest. 1953 Nov;32(11):1065–1069. doi: 10.1172/JCI102828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCullough H. Semi-automated method for the differential determination of plasma catecholamines. J Clin Pathol. 1968 Nov;21(6):759–763. doi: 10.1136/jcp.21.6.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy B. E. Some studies of the protein-binding of steroids and their application to the routine micro and ultramicro measurement of various steroids in body fluids by competitive protein-binding radioassay. J Clin Endocrinol Metab. 1967 Jul;27(7):973–990. doi: 10.1210/jcem-27-7-973. [DOI] [PubMed] [Google Scholar]
  13. REDGATE E. S., GELLHORN E. Nature of sympathetico-adrenal discharge under conditions of excitation of central autonomic structures. Am J Physiol. 1953 Sep;174(3):475–480. doi: 10.1152/ajplegacy.1953.174.3.475. [DOI] [PubMed] [Google Scholar]
  14. SCHALCH D. S., PARKER M. L. A SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA. Nature. 1964 Sep 12;203:1141–1142. doi: 10.1038/2031141a0. [DOI] [PubMed] [Google Scholar]
  15. TROUT D. L., ESTES E. H., Jr, FRIEDBERG S. J. Titration of free fatty acids of plasma: a study of current methods and a new modification. J Lipid Res. 1960 Apr;1:199–202. [PubMed] [Google Scholar]
  16. UNGER R. H., EISENTRAUT A. M., McCALL M. S., MADISON L. L. Measurements of endogenous glucagon in plasma and the influence of blood glucose concentration upon its secretion. J Clin Invest. 1962 Apr;41:682–689. doi: 10.1172/JCI104525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WALLACE J. M., HARLAN W. R. SIGNIFICANCE OF EPINEPHRINE IN INSULIN HYPOGLYCEMIA IN MAN. Am J Med. 1965 Apr;38:531–539. doi: 10.1016/0002-9343(65)90131-2. [DOI] [PubMed] [Google Scholar]
  18. Wegienka L. C., Grasso S. G., Forsham P. H. Estimation of adrenomedullary reserve by infusion of 2-deoxy-D-glucose. J Clin Endocrinol Metab. 1966 Jan;26(1):37–45. doi: 10.1210/jcem-26-1-37. [DOI] [PubMed] [Google Scholar]

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