Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1973 Oct;52(10):2486–2494. doi: 10.1172/JCI107439

Brush Border Disaccharidases in Dog Kidney and Their Spatial Relationship to Glucose Transport Receptors

Melvin Silverman 1
PMCID: PMC302507  PMID: 4729044

Abstract

The localization of disaccharidases in kidney has been studied by means of the multiple indicator dilution technique. A pulse injection of a solution containing Evans blue dye (plasma marker), creatinine (extracellular marker), and a 14C-labeled disaccharide (lactose, sucrose, maltose, and αα-trehalose), is made into the renal artery of an anesthetized dog, and the outflow curves are monitored simultaneously from renal venous and urine effluents. Lactose and sucrose have an extracellular distribution. Trehalose and maltose remain extracellular from the postglomerular circulation. About 75% of filtered tracer maltose or trehalose is extracted by the luminal surface of the nephron. Thin-layer chromatography of urine samples shows that all of the excreted 14C radiolabel is in the form of the injected disaccharide. Following the administration of phlorizin, all of the filtered radioactivity is recoverable in the urine, but chromatography of the urine samples now reveals that there is a significant excretion of [14C]glucose, approximating the amount previously extracted under control conditions (in the absence of phlorizin). It has been verified that no hydrolysis of maltose or trehalose to their constituent glucose subunits occurred during the transit of tracer between the point of injection (renal artery), and the point of filtration (glomerular basement membrane). Similarly, after addition of [14C]disaccharides to fresh urine there is no chromatographically recoverable [14C]glucose.

It is concluded that there exist α-glucosidases with maltase and trehalase activity along the brush border of the proximal tubule and that these disaccharidases are located spatially superficial to the glucose transport receptors.

Full text

PDF
2486

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger S. J., Sacktor B. Isolation and biochemical characterization of brush borders from rabbit kidney. J Cell Biol. 1970 Dec;47(3):637–645. doi: 10.1083/jcb.47.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHINARD F. P., TAYLOR W. R., NOLAN M. F., ENNS T. Renal handling of glucose in dogs. Am J Physiol. 1959 Mar;196(3):535–544. doi: 10.1152/ajplegacy.1959.196.3.535. [DOI] [PubMed] [Google Scholar]
  3. Chertok R. J., Lake S. A simple method for the preparation of renal brush borders. J Cell Biol. 1972 Aug;54(2):426–430. [PMC free article] [PubMed] [Google Scholar]
  4. Glossmann H., Neville D. M., Jr Phlorizin receptors in isolated kidney brush border membranes. J Biol Chem. 1972 Dec 10;247(23):7779–7789. [PubMed] [Google Scholar]
  5. Glossmann H., Neville D. M. gamma-Glutamyltransferase in kidney brush border membranes. FEBS Lett. 1972 Jan 1;19(4):340–344. doi: 10.1016/0014-5793(72)80075-9. [DOI] [PubMed] [Google Scholar]
  6. MILLER D., CRANE R. K. The digestive function of the epithelium of the small intestine. I. An intracellular locus of disaccharide and sugar phosphate ester hydrolysis. Biochim Biophys Acta. 1961 Sep 16;52:281–293. doi: 10.1016/0006-3002(61)90677-1. [DOI] [PubMed] [Google Scholar]
  7. MILLER D., CRANE R. K. The digestive function of the epithelium of the small intestine. II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells. Biochim Biophys Acta. 1961 Sep 16;52:293–298. doi: 10.1016/0006-3002(61)90678-3. [DOI] [PubMed] [Google Scholar]
  8. Malathi P., Crane R. K. Spatial relationship between intestinal disaccharidases and the active transport system for sugars. Biochim Biophys Acta. 1968 Sep 17;163(2):275–277. doi: 10.1016/0005-2736(68)90109-0. [DOI] [PubMed] [Google Scholar]
  9. Sacktor B. Trehalase and the transport of glucose in the mammalian kidney and intestine. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1007–1014. doi: 10.1073/pnas.60.3.1007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sacktor B., Wu N. C. Studies on the spatial relationship between intestinal disaccharidases and the phlorizin-sensitive transport of glucose. Arch Biochem Biophys. 1971 May;144(1):423–427. doi: 10.1016/0003-9861(71)90495-4. [DOI] [PubMed] [Google Scholar]
  11. Semenza G., Rihová L. Allosteric effects and phlorizin inhibition of intestinal trehalase. Biochim Biophys Acta. 1969 Apr 22;178(2):393–396. doi: 10.1016/0005-2744(69)90409-4. [DOI] [PubMed] [Google Scholar]
  12. Silverman M., Aganon M. A., Chinard F. P. D-glucose interactions with renal tubule cell surfaces. Am J Physiol. 1970 Mar;218(3):735–742. doi: 10.1152/ajplegacy.1970.218.3.735. [DOI] [PubMed] [Google Scholar]
  13. Silverman M., Aganon M. A., Chinard F. P. Specificity of monosaccharide transport in dog kidney. Am J Physiol. 1970 Mar;218(3):743–750. doi: 10.1152/ajplegacy.1970.218.3.743. [DOI] [PubMed] [Google Scholar]
  14. Starnes C. W., Welsh J. D. Intestinal sucrase-isomaltase deficiency and renal calculi. N Engl J Med. 1970 Apr 30;282(18):1023–1024. doi: 10.1056/NEJM197004302821809. [DOI] [PubMed] [Google Scholar]
  15. Stevenson F. K. The disaccharidase activity of a membrane fraction obtained from the rabbit renal cortex. Biochim Biophys Acta. 1972 Apr 14;266(1):144–153. doi: 10.1016/0005-2736(72)90130-7. [DOI] [PubMed] [Google Scholar]
  16. Young J. M., Weser E. The metabolism of circulating maltose in man. J Clin Invest. 1971 May;50(5):986–991. doi: 10.1172/JCI106592. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES