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The volume of the water droplet pre-deposited on the MALDI target plate changed from 0.5 µL to 2.0 µL. The sample contained 0.05 µg/µL horse myoglobin, 0.9% Pharmalyte, 2% CHAPS and 0.5 mM ammonia acetate. ~0.1 µL of the sample was delivered to the water droplet. All other conditions were the same as in Figures 2A and 2C. The error bars were obtained by repeating the same tests for three times, from CIEF separation to MS measurement.
