FIG 2 .

Northern analysis of predicted sRNAs. Validation of expression for a select set of sRNAs in C. acetobutylicum and C. botulinum by Northern analysis using single-stranded oligonucleotide probes. RNA from pool CAC2 was used for the C. acetobutylicum sRNAs, and RNA from pool CLK was used for the C. botulinum sRNAs. These RNA pools are described in Text S2 in the supplemental material. Ethidium bromide gels of 16S RNA are shown as qualitative and approximate loading controls. (A) Four predicted sRNAs from C. acetobutylicum (sCAC1449, sCAC1760, sCAC3821, and sCAC3340). (B) Two predicted sRNAs from C. botulinum A3 Loch Maree (sCLK_200 and sCLK_3105). (C) The predicted sCAC137 was validated with Northern analysis using a single-stranded oligonucleotide probe. The upstream annotated tRNA, CACt008, was also probed to confirm the tRNA size using a single-stranded oligonucleotide probe.