FIG 3 .

Expression of sCAC610 and CAC0528 in response to clindamycin treatment of C. acetobutylicum cultures. A single-stranded oligonucleotide probe was used in Northern analysis of sCAC610, and a double-stranded oligonucleotide probe was used for CAC0528. (A) Relative expression, by Q-RT-PCR analysis, of sCAC610 and CAC0528 upon clindamycin treatment, compared to untreated cells. The cells were treated with vehicle (no-clindamycin control) or with 50, 75, and 100 μg/ml clindamycin for 30 and 60 min. (B) Differential expression of sCAC610 and CAC0528 was confirmed using Northern analysis. The cells were treated with 50 µg/ml clindamycin for 30 and 60 min or not treated with clindamycin (0 µg/ml). Ethidium bromide gels of 16S RNA are shown as qualitative loading controls. (C) Predicted rho-independent terminators downstream of CAC0528. (D) DNA sequence of the entire intergenic region upstream of CAC0528. The sCAC610 transcript determined by the 5′ and 3′ RACE reactions is shown in bold type.