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. 1974 May;53(5):1250–1257. doi: 10.1172/JCI107671

The Effect of Cyclic AMP and Dibutyryl Cyclic AMP on the Permeability Characteristics of the Renal Tubule

William B Lorentz Jr 1
PMCID: PMC302611  PMID: 4363407

Abstract

The effect of adenosine-3′,5′-cyclic monophosphate (cyclic AMP) and N6,O2-dibutyryl adenosine-3′,5′-cyclic monophosphate (dibutyryl cyclic AMP) on renal tubular permeability was studied by microinjection techniques in anesthetized diuretic rats. Radioactive inulin and mannitol were microinjected simultaneously into superficial proximal and distal convolutions and recovery of the isotopes was measured in the urine.

During control conditions, mannitol and inulin recovery was essentially complete. However, during infusion of cyclic AMP or dibutyryl cyclic AMP, mannitol recovery was significantly less than control after early proximal and late proximal microinjections, averaging 79 and 85%, respectively. There was no loss of mannitol from the nephron after microinjection into distal convolutions. Inulin recovery was complete after all microinjections during cyclic AMP or dibutyryl cyclic AMP infusion. Simultaneous clearances of mannitol and inulin as well as peritubular capillary microinjections studies demonstrated bidirectional fluxes of mannitol across the proximal tubular epithelium during infusion of cyclic AMP or dibutyryl cyclic AMP. Intratubular pressures were not different during control and experimental periods.

These studies demonstrate a change in the permeability characteristic of the proximal convoluted tubule during infusion of cyclic AMP and dibutyryl cyclic AMP. This change in permeability of the proximal tubule could account for the effects of cyclic AMP on proximal tubular transport processes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agus Z. S., Puschett J. B., Senesky D., Goldberg M. Mode of action of parathyroid hormone and cyclic adenosine 3',5'-monophosphate on renal tubular phosphate reabsorption in the dog. J Clin Invest. 1971 Mar;50(3):617–626. doi: 10.1172/JCI106532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boulpaep E. L. Permeability changes of the proximal tubule of Necturus during saline loading. Am J Physiol. 1972 Mar;222(3):517–531. doi: 10.1152/ajplegacy.1972.222.3.517. [DOI] [PubMed] [Google Scholar]
  3. Boulpaep E. L., Seely J. F. Electrophysiology of proximal and distal tubules in the autoperfused dog kidney. Am J Physiol. 1971 Oct;221(4):1084–1096. doi: 10.1152/ajplegacy.1971.221.4.1084. [DOI] [PubMed] [Google Scholar]
  4. Butlen D., Jard S. Renal handling of 3'-5'-cyclic AMP in the rat. The possible role of luminal 3'-5'-cyclic AMP in the tubular reabsorption of phosphate. Pflugers Arch. 1972;331(2):172–190. doi: 10.1007/BF00587260. [DOI] [PubMed] [Google Scholar]
  5. DIAMOND J. M. The mechanism of solute transport by the gall-bladder. J Physiol. 1962 May;161:474–502. doi: 10.1113/jphysiol.1962.sp006899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fraser D., Kooh S. W., Scriver C. R. Hyperparathyroidism as the cause of hyperaminoaciduria and phosphaturia in human vitamin D deficiency. Pediatr Res. 1967 Nov;1(6):425–435. doi: 10.1203/00006450-196711000-00001. [DOI] [PubMed] [Google Scholar]
  7. GOTTSCHALK C. W., MOREL F., MYLLE M. TRACER MICROINJECTION STUDIES OF RENAL TUBULAR PERMEABILITY. Am J Physiol. 1965 Jul;209:173–178. doi: 10.1152/ajplegacy.1965.209.1.173. [DOI] [PubMed] [Google Scholar]
  8. GOTTSCHALK C. W., MYLLE M. Micropuncture study of pressures in proximal tubules and peritubular capillaries of the rat kidney and their relation to ureteral and renal venous pressures. Am J Physiol. 1956 May;185(2):430–439. doi: 10.1152/ajplegacy.1956.185.2.430. [DOI] [PubMed] [Google Scholar]
  9. GUTMAN Y., GOTTSCHALK C. W., LASSITER W. E. MICROPUNCTURE STUDY OF INULIN ABSORPTION IN THE RAT KIDNEY. Science. 1965 Feb 12;147(3659):753–754. doi: 10.1126/science.147.3659.753. [DOI] [PubMed] [Google Scholar]
  10. Giebisch G. Functional organization of proximal and distal tubular electrolyte transport. Nephron. 1969;6(3):260–281. doi: 10.1159/000179733. [DOI] [PubMed] [Google Scholar]
  11. Grose J. H., Scriver C. R. Parathyroid-dependent phosphaturia and aminoaciduria in the vitamin D-deficient rat. Am J Physiol. 1968 Feb;214(2):370–377. doi: 10.1152/ajplegacy.1968.214.2.370. [DOI] [PubMed] [Google Scholar]
  12. Henion W. F., Sutherland E. W., Posternak T. Effects of derivatives of adenosine 3',5'-phosphate on liver slices and intact animals. Biochim Biophys Acta. 1967 Oct 9;148(1):106–113. doi: 10.1016/0304-4165(67)90284-x. [DOI] [PubMed] [Google Scholar]
  13. Liddle G. W., Hardman J. G. Cyclic adenosine monophosphate as a mediator of hormone action. N Engl J Med. 1971 Sep 2;285(10):560–566. doi: 10.1056/NEJM197109022851007. [DOI] [PubMed] [Google Scholar]
  14. Linarelli L. G. Newborn urinary cyclic AMP and developmental renal responsiveness to parathyroid hormone. Pediatrics. 1972 Jul;50(1):14–23. [PubMed] [Google Scholar]
  15. Lorentz W. B., Jr, Lassiter W. E., Gottschalk C. W. Renal tubular permeability during increased intrarenal pressure. J Clin Invest. 1972 Mar;51(3):484–492. doi: 10.1172/JCI106836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MARSH D., FRASIER C. RELIABILITY OF INULIN FOR DETERMINING VOLUME FLOW IN RAT RENAL CORTICAL TUBULES. Am J Physiol. 1965 Aug;209:283–286. doi: 10.1152/ajplegacy.1965.209.2.283. [DOI] [PubMed] [Google Scholar]
  17. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Streeto J. M. Renal cortical adenyl cyclase: effect of parathyroid hormone and calcium. Metabolism. 1969 Nov;18(11):968–973. doi: 10.1016/0026-0495(69)90037-7. [DOI] [PubMed] [Google Scholar]
  19. Ussing H. H., Johansen B. Anomalous transport of sucrose and urea in toad skin. Nephron. 1969;6(3):317–328. doi: 10.1159/000179736. [DOI] [PubMed] [Google Scholar]
  20. WINBERG J., BERGSTROEM T. RENAL ACIDIFICATION DEFECT IN INFANTS WITH MILD DEFICIENCY RICKETS. Acta Paediatr Scand. 1965 Mar;54:139–148. doi: 10.1111/j.1651-2227.1965.tb06355.x. [DOI] [PubMed] [Google Scholar]

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