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The TORC1 signalling pathway regulates nuclear re-export of retrogradely transported spliced tRNAs, but not nuclear export of tRNAs made from intronless pre-tRNAs. The S. cerevisiae TB50a strain was treated with drug delivery solution (90% ethanol and 10% Tween 20) with or without 200 ng/ml rapamycin in YPD medium for the times indicated and FISH was used to monitor the cellular location of tRNATyr and tRNAGly.
