Figure 5.

Intranuclear membranes of oocyte nuclei contain luminal and integral membrane ER marker proteins. Nuclear envelope spreads of Xenopus oocytes were stained for FLAG-tagged lamin B2 and detected by indirect immunofluorescence with mAb M2 and a Cy3-conjugated secondary antibody (Lamin) and analyzed by confocal fluorescence microscopy. GFP was detected in the green channel. (A) Spreads of oocytes co-expressing lamin B2 and GFP-Sec61γ, (B) GFP-Sec61γ alone, (C) co-expressing lamin B2 and the luminal ER-GFP, (D) ER-GFP alone, (E) spreads of non-injected control oocytes. The right parts show merged images of the two channels. Size bar for all parts 10 µm.