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. 1993 Jan;61(1):364–367. doi: 10.1128/iai.61.1.364-367.1993

Acid resistance in enteric bacteria.

J Gorden 1, P L Small 1
PMCID: PMC302732  PMID: 8418063

Abstract

Shigella species require a uniquely small inoculum for causing dysentery. One explanation for the low infective dose is that Shigella species are better able to survive the acidic conditions encountered in the stomach than are other enteric pathogens. We have tested Shigella species, Escherichia coli, and Salmonella species for the ability to survive at pH 2.5 for at least 2 h. Most isolates of Shigella and E. coli survived this treatment, whereas none of the Salmonella isolates were able to do so. The ability of Shigella species to survive at low pHs does not require the presence of the large virulence plasmid or growth at 37 degrees C but is strikingly dependent on growth phase. We have also found that Shigella isolates exposed to acid lose the ability to invade epithelial cells.

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Selected References

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  1. Blaser M. J., Newman L. S. A review of human salmonellosis: I. Infective dose. Rev Infect Dis. 1982 Nov-Dec;4(6):1096–1106. doi: 10.1093/clinids/4.6.1096. [DOI] [PubMed] [Google Scholar]
  2. Bloch C. A., Stocker B. A., Orndorff P. E. A key role for type 1 pili in enterobacterial communicability. Mol Microbiol. 1992 Mar;6(6):697–701. doi: 10.1111/j.1365-2958.1992.tb01518.x. [DOI] [PubMed] [Google Scholar]
  3. Bohannon D. E., Connell N., Keener J., Tormo A., Espinosa-Urgel M., Zambrano M. M., Kolter R. Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70. J Bacteriol. 1991 Jul;173(14):4482–4492. doi: 10.1128/jb.173.14.4482-4492.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cash R. A., Music S. I., Libonati J. P., Snyder M. J., Wenzel R. P., Hornick R. B. Response of man to infection with Vibrio cholerae. I. Clinical, serologic, and bacteriologic responses to a known inoculum. J Infect Dis. 1974 Jan;129(1):45–52. doi: 10.1093/infdis/129.1.45. [DOI] [PubMed] [Google Scholar]
  5. Drasar B. S., Shiner M., McLeod G. M. Studies on the intestinal flora. I. The bacterial flora of the gastrointestinal tract in healthy and achlorhydric persons. Gastroenterology. 1969 Jan;56(1):71–79. [PubMed] [Google Scholar]
  6. DuPont H. L., Formal S. B., Hornick R. B., Snyder M. J., Libonati J. P., Sheahan D. G., LaBrec E. H., Kalas J. P. Pathogenesis of Escherichia coli diarrhea. N Engl J Med. 1971 Jul 1;285(1):1–9. doi: 10.1056/NEJM197107012850101. [DOI] [PubMed] [Google Scholar]
  7. DuPont H. L., Hornick R. B., Snyder M. J., Libonati J. P., Formal S. B., Gangarosa E. J. Immunity in shigellosis. I. Response of man to attenuated strains of Shigella. J Infect Dis. 1972 Jan;125(1):5–11. doi: 10.1093/infdis/125.1.5. [DOI] [PubMed] [Google Scholar]
  8. DuPont H. L., Levine M. M., Hornick R. B., Formal S. B. Inoculum size in shigellosis and implications for expected mode of transmission. J Infect Dis. 1989 Jun;159(6):1126–1128. doi: 10.1093/infdis/159.6.1126. [DOI] [PubMed] [Google Scholar]
  9. Foster J. W., Hall H. K. Adaptive acidification tolerance response of Salmonella typhimurium. J Bacteriol. 1990 Feb;172(2):771–778. doi: 10.1128/jb.172.2.771-778.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Giannella R. A., Broitman S. A., Zamcheck N. Influence of gastric acidity on bacterial and parasitic enteric infections. A perspective. Ann Intern Med. 1973 Feb;78(2):271–276. doi: 10.7326/0003-4819-78-2-271. [DOI] [PubMed] [Google Scholar]
  11. Hale T. L. Genetic basis of virulence in Shigella species. Microbiol Rev. 1991 Jun;55(2):206–224. doi: 10.1128/mr.55.2.206-224.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hale T. L., Oaks E. V., Formal S. B. Identification and antigenic characterization of virulence-associated, plasmid-coded proteins of Shigella spp. and enteroinvasive Escherichia coli. Infect Immun. 1985 Dec;50(3):620–629. doi: 10.1128/iai.50.3.620-629.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Herrington D. A., Van de Verg L., Formal S. B., Hale T. L., Tall B. D., Cryz S. J., Tramont E. C., Levine M. M. Studies in volunteers to evaluate candidate Shigella vaccines: further experience with a bivalent Salmonella typhi-Shigella sonnei vaccine and protection conferred by previous Shigella sonnei disease. Vaccine. 1990 Aug;8(4):353–357. doi: 10.1016/0264-410x(90)90094-3. [DOI] [PubMed] [Google Scholar]
  14. Hornick R. B., Greisman S. E., Woodward T. E., DuPont H. L., Dawkins A. T., Snyder M. J. Typhoid fever: pathogenesis and immunologic control. 2. N Engl J Med. 1970 Oct 1;283(14):739–746. doi: 10.1056/NEJM197010012831406. [DOI] [PubMed] [Google Scholar]
  15. Hromockyj A. E., Maurelli A. T. Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence. J Bacteriol. 1989 May;171(5):2879–2881. doi: 10.1128/jb.171.5.2879-2881.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jerse A. E., Gicquelais K. G., Kaper J. B. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli. Infect Immun. 1991 Nov;59(11):3869–3875. doi: 10.1128/iai.59.11.3869-3875.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Keusch G. T., Bennish M. L. Shigellosis: recent progress, persisting problems and research issues. Pediatr Infect Dis J. 1989 Oct;8(10):713–719. [PubMed] [Google Scholar]
  18. Lange R., Hengge-Aronis R. Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):49–59. doi: 10.1111/j.1365-2958.1991.tb01825.x. [DOI] [PubMed] [Google Scholar]
  19. Levine M. M., Hone D., Tacket C., Ferreccio C., Cryz S. Clinical and field trials with attenuated Salmonella typhi as live oral vaccines and as "carrier" vaccines. Res Microbiol. 1990 Sep-Oct;141(7-8):807–816. doi: 10.1016/0923-2508(90)90114-6. [DOI] [PubMed] [Google Scholar]
  20. McCann M. P., Kidwell J. P., Matin A. The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli. J Bacteriol. 1991 Jul;173(13):4188–4194. doi: 10.1128/jb.173.13.4188-4194.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peterson W. L., Mackowiak P. A., Barnett C. C., Marling-Cason M., Haley M. L. The human gastric bactericidal barrier: mechanisms of action, relative antibacterial activity, and dietary influences. J Infect Dis. 1989 May;159(5):979–983. doi: 10.1093/infdis/159.5.979. [DOI] [PubMed] [Google Scholar]
  22. Riley L. W., Junio L. N., Libaek L. B., Schoolnik G. K. Plasmid-encoded expression of lipopolysaccharide O-antigenic polysaccharide in enteropathogenic Escherichia coli. Infect Immun. 1987 Sep;55(9):2052–2056. doi: 10.1128/iai.55.9.2052-2056.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Small P. L., Isberg R. R., Falkow S. Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells. Infect Immun. 1987 Jul;55(7):1674–1679. doi: 10.1128/iai.55.7.1674-1679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Speelman P., Kabir I., Islam M. Distribution and spread of colonic lesions in shigellosis: a colonoscopic study. J Infect Dis. 1984 Dec;150(6):899–903. doi: 10.1093/infdis/150.6.899. [DOI] [PubMed] [Google Scholar]
  25. Taylor D. N., Echeverria P., Sethabutr O., Pitarangsi C., Leksomboon U., Blacklow N. R., Rowe B., Gross R., Cross J. Clinical and microbiologic features of Shigella and enteroinvasive Escherichia coli infections detected by DNA hybridization. J Clin Microbiol. 1988 Jul;26(7):1362–1366. doi: 10.1128/jcm.26.7.1362-1366.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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