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. 2011 Jan 21;60(2):436–442. doi: 10.2337/db10-0352

FIG. 2.

FIG. 2.

Total and phosphorylated forms of AMPKα in three hindbrain regions after 2DG injection. Tissue homogenates were obtained 45 or 120 min after saline (control) or 2DG (200 mg/kg s.c.) injection. Equivalent amounts of protein were subjected to SDS-PAGE and immunoblotted with anti-pAMPKα, anti-total AMPKα, or anti–β-actin antibody. A: Upper panel: immunoblots of pAMPKα and β-actin in A1–C1m, VMM, and A2–C2 regions with saline or 2DG injection. Lower panel: quantitative data. B: Upper panel: immunoblots of total AMPKα and β-actin in A1–C1m and A2–C2 regions. Lower panel: quantitative data. To compare expression levels of these proteins, the intensity of each band was quantified and normalized with β-actin and expressed as the percentage of saline control rats. *P < 0.01 vs. the control saline-injected rats. Fisher PLSD test after one-way ANOVA (n = eight rats for each group).