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. 1994 Jul;62(7):2995–2997. doi: 10.1128/iai.62.7.2995-2997.1994

Growth temperature reversibly modulates the virulence of Legionella pneumophila.

W S Mauchline 1, B W James 1, R B Fitzgeorge 1, P J Dennis 1, C W Keevil 1
PMCID: PMC302910  PMID: 8005687

Abstract

In chemostat culture, the virulence of two strains of Legionella pneumophila was shown to be significantly (P < 0.05) reduced when the culture temperature was lowered from 37 to 24 degrees C. This modulation was reversed by returning the temperature to 37 degrees C, which resulted in a statistically significant (P < 0.05) increase in virulence.

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Selected References

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  1. Baskerville A., Fitzgeorge R. B., Broster M., Hambleton P., Dennis P. J. Experimental transmission of legionnaires' disease by exposure to aerosols of Legionella pneumophila. Lancet. 1981 Dec 19;2(8260-61):1389–1390. doi: 10.1016/s0140-6736(81)92803-8. [DOI] [PubMed] [Google Scholar]
  2. Berg J. D., Hoff J. C., Roberts P. V., Matin A. Growth of Legionella pneumophila in continuous culture. Appl Environ Microbiol. 1985 Jun;49(6):1534–1537. doi: 10.1128/aem.49.6.1534-1537.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catrenich C. E., Johnson W. Virulence conversion of Legionella pneumophila: a one-way phenomenon. Infect Immun. 1988 Dec;56(12):3121–3125. doi: 10.1128/iai.56.12.3121-3125.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colville A., Crowley J., Dearden D., Slack R. C., Lee J. V. Outbreak of Legionnaires' disease at University Hospital, Nottingham. Epidemiology, microbiology and control. Epidemiol Infect. 1993 Feb;110(1):105–116. doi: 10.1017/s0950268800050731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edelstein P. H., Beer K. B., DeBoynton E. D. Influence of growth temperature on virulence of Legionella pneumophila. Infect Immun. 1987 Nov;55(11):2701–2705. doi: 10.1128/iai.55.11.2701-2705.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol. 1981 Sep;14(3):298–303. doi: 10.1128/jcm.14.3.298-303.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fitzgeorge R. B., Baskerville A., Broster M., Hambleton P., Dennis P. J. Aerosol infection of animals with strains of Legionella pneumophila of different virulence: comparison with intraperitoneal and intranasal routes of infection. J Hyg (Lond) 1983 Feb;90(1):81–89. doi: 10.1017/s0022172400063877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fliermans C. B., Cherry W. B., Orrison L. H., Smith S. J., Tison D. L., Pope D. H. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol. 1981 Jan;41(1):9–16. doi: 10.1128/aem.41.1.9-16.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gross R. Signal transduction and virulence regulation in human and animal pathogens. FEMS Microbiol Rev. 1993 Apr;10(3-4):301–326. doi: 10.1111/j.1574-6968.1993.tb05873.x. [DOI] [PubMed] [Google Scholar]
  10. HERBERT D., ELSWORTH R., TELLING R. C. The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol. 1956 Jul;14(3):601–622. doi: 10.1099/00221287-14-3-601. [DOI] [PubMed] [Google Scholar]
  11. LACEY B. W. Antigenic modulation of Bordetella pertussis. J Hyg (Lond) 1960 Mar;58:57–93. doi: 10.1017/s0022172400038134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leimeister-Wächter M., Domann E., Chakraborty T. The expression of virulence genes in Listeria monocytogenes is thermoregulated. J Bacteriol. 1992 Feb;174(3):947–952. doi: 10.1128/jb.174.3.947-952.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mauchline W. S., Araujo R., Wait R., Dowsett A. B., Dennis P. J., Keevil C. W. Physiology and morphology of Legionella pneumophila in continuous culture at low oxygen concentration. J Gen Microbiol. 1992 Nov;138(11):2371–2380. doi: 10.1099/00221287-138-11-2371. [DOI] [PubMed] [Google Scholar]
  14. Maurelli A. T., Blackmon B., Curtiss R., 3rd Temperature-dependent expression of virulence genes in Shigella species. Infect Immun. 1984 Jan;43(1):195–201. doi: 10.1128/iai.43.1.195-201.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maurelli A. T. Temperature regulation of virulence genes in pathogenic bacteria: a general strategy for human pathogens? Microb Pathog. 1989 Jul;7(1):1–10. doi: 10.1016/0882-4010(89)90106-x. [DOI] [PubMed] [Google Scholar]
  16. McDade J. E., Shepard C. C. Virulent to avirulent conversion of Legionnaires' disease bacterium (Legionella pneumophila)--its effect on isolation techniques. J Infect Dis. 1979 Jun;139(6):707–711. doi: 10.1093/infdis/139.6.707. [DOI] [PubMed] [Google Scholar]
  17. Miller J. F., Mekalanos J. J., Falkow S. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science. 1989 Feb 17;243(4893):916–922. doi: 10.1126/science.2537530. [DOI] [PubMed] [Google Scholar]
  18. NOVICK A., SZILARD L. Experiments with the Chemostat on spontaneous mutations of bacteria. Proc Natl Acad Sci U S A. 1950 Dec;36(12):708–719. doi: 10.1073/pnas.36.12.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ott M., Messner P., Heesemann J., Marre R., Hacker J. Temperature-dependent expression of flagella in Legionella. J Gen Microbiol. 1991 Aug;137(8):1955–1961. doi: 10.1099/00221287-137-8-1955. [DOI] [PubMed] [Google Scholar]
  20. POWELL E. O. Criteria for the growth of contaminants and mutants in continuous culture. J Gen Microbiol. 1958 Feb;18(1):259–268. doi: 10.1099/00221287-18-1-259. [DOI] [PubMed] [Google Scholar]
  21. Pine L., Hoffman P. S., Malcolm G. B., Benson R. F., Franzus M. J. Role of keto acids and reduced-oxygen-scavenging enzymes in the growth of Legionella species. J Clin Microbiol. 1986 Jan;23(1):33–42. doi: 10.1128/jcm.23.1.33-42.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Plouffe J. F., Webster L. R., Hackman B. Relationship between colonization of hospital building with Legionella pneumophila and hot water temperatures. Appl Environ Microbiol. 1983 Sep;46(3):769–770. doi: 10.1128/aem.46.3.769-770.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Recsei P., Kreiswirth B., O'Reilly M., Schlievert P., Gruss A., Novick R. P. Regulation of exoprotein gene expression in Staphylococcus aureus by agar. Mol Gen Genet. 1986 Jan;202(1):58–61. doi: 10.1007/BF00330517. [DOI] [PubMed] [Google Scholar]
  24. Straley S. C., Bowmer W. S. Virulence genes regulated at the transcriptional level by Ca2+ in Yersinia pestis include structural genes for outer membrane proteins. Infect Immun. 1986 Feb;51(2):445–454. doi: 10.1128/iai.51.2.445-454.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wadowsky R. M., Wolford R., McNamara A. M., Yee R. B. Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water. Appl Environ Microbiol. 1985 May;49(5):1197–1205. doi: 10.1128/aem.49.5.1197-1205.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yamamoto H., Sugiura M., Kusunoki S., Ezaki T., Ikedo M., Yabuuchi E. Factors stimulating propagation of legionellae in cooling tower water. Appl Environ Microbiol. 1992 Apr;58(4):1394–1397. doi: 10.1128/aem.58.4.1394-1397.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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