Abstract
Wild-type Bordetella bronchiseptica and a bvg mutant strain were used for invasion and survival experiments in human Caco-2 and A549 epithelial cells. Both bacterial strains were able to enter and persist within the host cells for at least a week. A significant proportion of the bacteria from both B. bronchiseptica strains but not from Bordetella pertussis were found free in the cytoplasm, suggesting different invasion and survival strategies of the two species in epithelial cells.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CRAWFORD J. G., FISHEL C. W. Growth of Bordetella pertussis in tissue culture. J Bacteriol. 1959 Apr;77(4):465–474. doi: 10.1128/jb.77.4.465-474.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carbonetti N. H., Khelef N., Guiso N., Gross R. A phase variant of Bordetella pertussis with a mutation in a new locus involved in the regulation of pertussis toxin and adenylate cyclase toxin expression. J Bacteriol. 1993 Oct;175(20):6679–6688. doi: 10.1128/jb.175.20.6679-6688.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheers C., Gray D. F. Macrophage behaviour during the complaisant phase of murine pertussis. Immunology. 1969 Dec;17(6):875–887. [PMC free article] [PubMed] [Google Scholar]
- Ewanowich C. A., Melton A. R., Weiss A. A., Sherburne R. K., Peppler M. S. Invasion of HeLa 229 cells by virulent Bordetella pertussis. Infect Immun. 1989 Sep;57(9):2698–2704. doi: 10.1128/iai.57.9.2698-2704.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghosh H. K., Tranter J. Bordetella bronchicanis (bronchiseptica) infection in man: review and a case report. J Clin Pathol. 1979 Jun;32(6):546–548. doi: 10.1136/jcp.32.6.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg M. B., Sansonetti P. J. Shigella subversion of the cellular cytoskeleton: a strategy for epithelial colonization. Infect Immun. 1993 Dec;61(12):4941–4946. doi: 10.1128/iai.61.12.4941-4946.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gross R., Aricò B., Rappuoli R. Families of bacterial signal-transducing proteins. Mol Microbiol. 1989 Nov;3(11):1661–1667. doi: 10.1111/j.1365-2958.1989.tb00152.x. [DOI] [PubMed] [Google Scholar]
- Gross R., Rappuoli R. Pertussis toxin promoter sequences involved in modulation. J Bacteriol. 1989 Jul;171(7):4026–4030. doi: 10.1128/jb.171.7.4026-4030.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heesemann J., Laufs R. Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture. J Clin Microbiol. 1985 Aug;22(2):168–175. doi: 10.1128/jcm.22.2.168-175.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karunasagar I., Krohne G., Goebel W. Listeria ivanovii is capable of cell-to-cell spread involving actin polymerization. Infect Immun. 1993 Jan;61(1):162–169. doi: 10.1128/iai.61.1.162-169.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee C. K., Roberts A. L., Finn T. M., Knapp S., Mekalanos J. J. A new assay for invasion of HeLa 229 cells by Bordetella pertussis: effects of inhibitors, phenotypic modulation, and genetic alterations. Infect Immun. 1990 Aug;58(8):2516–2522. doi: 10.1128/iai.58.8.2516-2522.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leininger E., Ewanowich C. A., Bhargava A., Peppler M. S., Kenimer J. G., Brennan M. J. Comparative roles of the Arg-Gly-Asp sequence present in the Bordetella pertussis adhesins pertactin and filamentous hemagglutinin. Infect Immun. 1992 Jun;60(6):2380–2385. doi: 10.1128/iai.60.6.2380-2385.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masure H. R. The adenylate cyclase toxin contributes to the survival of Bordetella pertussis within human macrophages. Microb Pathog. 1993 Apr;14(4):253–260. doi: 10.1006/mpat.1993.1025. [DOI] [PubMed] [Google Scholar]
- Relman D., Tuomanen E., Falkow S., Golenbock D. T., Saukkonen K., Wright S. D. Recognition of a bacterial adhesion by an integrin: macrophage CR3 (alpha M beta 2, CD11b/CD18) binds filamentous hemagglutinin of Bordetella pertussis. Cell. 1990 Jun 29;61(7):1375–1382. doi: 10.1016/0092-8674(90)90701-f. [DOI] [PubMed] [Google Scholar]
- Saukkonen K., Cabellos C., Burroughs M., Prasad S., Tuomanen E. Integrin-mediated localization of Bordetella pertussis within macrophages: role in pulmonary colonization. J Exp Med. 1991 May 1;173(5):1143–1149. doi: 10.1084/jem.173.5.1143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Savelkoul P. H., Kremer B., Kusters J. G., van der Zeijst B. A., Gaastra W. Invasion of HeLa cells by Bordetella bronchiseptica. Microb Pathog. 1993 Feb;14(2):161–168. doi: 10.1006/mpat.1993.1016. [DOI] [PubMed] [Google Scholar]
- Steed L. L., Setareh M., Friedman R. L. Intracellular survival of virulent Bordetella pertussis in human polymorphonuclear leukocytes. J Leukoc Biol. 1991 Oct;50(4):321–330. doi: 10.1002/jlb.50.4.321. [DOI] [PubMed] [Google Scholar]
- Weiss A. A., Hewlett E. L. Virulence factors of Bordetella pertussis. Annu Rev Microbiol. 1986;40:661–686. doi: 10.1146/annurev.mi.40.100186.003305. [DOI] [PubMed] [Google Scholar]
- Woolfrey B. F., Moody J. A. Human infections associated with Bordetella bronchiseptica. Clin Microbiol Rev. 1991 Jul;4(3):243–255. doi: 10.1128/cmr.4.3.243. [DOI] [PMC free article] [PubMed] [Google Scholar]