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. 1994 Jul;62(7):3022–3026. doi: 10.1128/iai.62.7.3022-3026.1994

Effect of Lewis blood group antigen expression on bacterial adherence to COS-1 cells.

R A Gaffney 1, A J Schaeffer 1, B E Anderson 1, J L Duncan 1
PMCID: PMC302916  PMID: 8005692

Abstract

Epithelial cells from secretor individuals demonstrate decreased bacterial adherence compared with cells from nonsecretors. Lewis blood group antigen expression is one component of the secretor/nonsecretor phenotype and several epidemiologic studies have suggested a link between Lewis blood group antigen phenotype and susceptibility to urinary tract infections. In this study, we examined the possibility that the expression of the difucosylated Lewis blood group determinants, Leb and Ley (associated with the secretor phenotype), made cells less susceptible to Escherichia coli adherence by masking receptors for pili. COS-1 cells, which do not produce Lewis (Lea, Leb, Le(x), and Ley) blood group antigens, were used as target cells for bacterial adherence. The surface blood group antigen expression pattern of the cells was then modified by cotransfection with plasmids containing DNA inserts encoding alpha (1,2)-fucosyltransferase and alpha (1,3)- and alpha (1,4)-fucosyltransferases, resulting in the expression of Leb and Ley. E. coli HB101 expressing various adhesins (type 1, PapJ96, PapIA2, PapAD110, Prs, and S) from recombinant plasmids bound equally well to untransfected cells and transfected cells expressing Lea and Le(x) (nonsecretor phenotype) and Leb and Ley (secretor phenotype) antigens. We conclude that the presence of Leb and Ley antigens on cells from secretors does not alone mask receptors for E. coli pili or hinder bacterial adherence.

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Selected References

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  1. Carroll S. M., Higa H. H., Paulson J. C. Different cell-surface receptor determinants of antigenically similar influenza virus hemagglutinins. J Biol Chem. 1981 Aug 25;256(16):8357–8363. [PubMed] [Google Scholar]
  2. Clegg S. Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6. Infect Immun. 1982 Nov;38(2):739–744. doi: 10.1128/iai.38.2.739-744.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cordon-Cardo C., Lloyd K. O., Finstad C. L., McGroarty M. E., Reuter V. E., Bander N. H., Old L. J., Melamed M. R. Immunoanatomic distribution of blood group antigens in the human urinary tract. Influence of secretor status. Lab Invest. 1986 Oct;55(4):444–454. [PubMed] [Google Scholar]
  4. Falk P., Roth K. A., Borén T., Westblom T. U., Gordon J. I., Normark S. An in vitro adherence assay reveals that Helicobacter pylori exhibits cell lineage-specific tropism in the human gastric epithelium. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2035–2039. doi: 10.1073/pnas.90.5.2035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fukuda M., Guan J. L., Rose J. K. A membrane-anchored form but not the secretory form of human chorionic gonadotropin-alpha chain acquires polylactosaminoglycan. J Biol Chem. 1988 Apr 15;263(11):5314–5318. [PubMed] [Google Scholar]
  6. Gaffney R. A., Schaeffer A. J., Duncan J. L. Lewis blood group antigen expression by cultured normal ureteral epithelial cells. J Urol. 1992 Oct;148(4):1341–1346. doi: 10.1016/s0022-5347(17)36905-7. [DOI] [PubMed] [Google Scholar]
  7. Gius D., Laimins L. A. Activation of human papillomavirus type 18 gene expression by herpes simplex virus type 1 viral transactivators and a phorbol ester. J Virol. 1989 Feb;63(2):555–563. doi: 10.1128/jvi.63.2.555-563.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hacker J., Schmidt G., Hughes C., Knapp S., Marget M., Goebel W. Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain. Infect Immun. 1985 Feb;47(2):434–440. doi: 10.1128/iai.47.2.434-440.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hultgren S. J., Porter T. N., Schaeffer A. J., Duncan J. L. Role of type 1 pili and effects of phase variation on lower urinary tract infections produced by Escherichia coli. Infect Immun. 1985 Nov;50(2):370–377. doi: 10.1128/iai.50.2.370-377.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johanson I., Lindstedt R., Svanborg C. Roles of the pap- and prs-encoded adhesins in Escherichia coli adherence to human uroepithelial cells. Infect Immun. 1992 Aug;60(8):3416–3422. doi: 10.1128/iai.60.8.3416-3422.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kinane D. F., Blackwell C. C., Brettle R. P., Weir D. M., Winstanley F. P., Elton R. A. ABO blood group, secretor state, and susceptibility to recurrent urinary tract infection in women. Br Med J (Clin Res Ed) 1982 Jul 3;285(6334):7–9. doi: 10.1136/bmj.285.6334.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kukowska-Latallo J. F., Larsen R. D., Nair R. P., Lowe J. B. A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase. Genes Dev. 1990 Aug;4(8):1288–1303. doi: 10.1101/gad.4.8.1288. [DOI] [PubMed] [Google Scholar]
  13. Larsen R. D., Ernst L. K., Nair R. P., Lowe J. B. Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6674–6678. doi: 10.1073/pnas.87.17.6674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Limas C. Detection of urothelial Lewis antigens with monoclonal antibodies. Am J Pathol. 1986 Dec;125(3):515–523. [PMC free article] [PubMed] [Google Scholar]
  15. Lindstedt R., Baker N., Falk P., Hull R., Hull S., Karr J., Leffler H., Svanborg Edén C., Larson G. Binding specificities of wild-type and cloned Escherichia coli strains that recognize globo-A. Infect Immun. 1989 Nov;57(11):3389–3394. doi: 10.1128/iai.57.11.3389-3394.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindstedt R., Larson G., Falk P., Jodal U., Leffler H., Svanborg C. The receptor repertoire defines the host range for attaching Escherichia coli strains that recognize globo-A. Infect Immun. 1991 Mar;59(3):1086–1092. doi: 10.1128/iai.59.3.1086-1092.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lloyd K. O. Philip Levine award lecture. Blood group antigens as markers for normal differentiation and malignant change in human tissues. Am J Clin Pathol. 1987 Jan;87(1):129–139. doi: 10.1093/ajcp/87.1.129. [DOI] [PubMed] [Google Scholar]
  18. Lomberg H., Cedergren B., Leffler H., Nilsson B., Carlström A. S., Svanborg-Edén C. Influence of blood group on the availability of receptors for attachment of uropathogenic Escherichia coli. Infect Immun. 1986 Mar;51(3):919–926. doi: 10.1128/iai.51.3.919-926.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lomberg H., Hellström M., Jodal U., Svanborg Eden C. Secretor state and renal scarring in girls with recurrent pyelonephritis. FEMS Microbiol Immunol. 1989 Jun;1(6-7):371–375. doi: 10.1111/j.1574-6968.1989.tb02423.x. [DOI] [PubMed] [Google Scholar]
  20. Lund B., Marklund B. I., Strömberg N., Lindberg F., Karlsson K. A., Normark S. Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities. Mol Microbiol. 1988 Mar;2(2):255–263. doi: 10.1111/j.1365-2958.1988.tb00027.x. [DOI] [PubMed] [Google Scholar]
  21. Mehta N. G. ABO(H) blood group antigens of the human erythrocyte membrane: contribution of glycoprotein and glycolipid. J Membr Biol. 1980 Jan 31;52(1):17–24. doi: 10.1007/BF01869002. [DOI] [PubMed] [Google Scholar]
  22. Navas E. L., Venegas M. F., Duncan J. L., Anderson B. E., Chmiel J. S., Schaeffer A. J. Blood group antigen expression on vaginal and buccal epithelial cells and mucus in secretor and nonsecretor women. J Urol. 1993 Jun;149(6):1492–1498. doi: 10.1016/s0022-5347(17)36425-x. [DOI] [PubMed] [Google Scholar]
  23. Normark S., Lark D., Hull R., Norgren M., Båga M., O'Hanley P., Schoolnik G., Falkow S. Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain. Infect Immun. 1983 Sep;41(3):942–949. doi: 10.1128/iai.41.3.942-949.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rajan V. P., Larsen R. D., Ajmera S., Ernst L. K., Lowe J. B. A cloned human DNA restriction fragment determines expression of a GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase in transfected cells. Evidence for isolation and transfer of the human H blood group locus. J Biol Chem. 1989 Jul 5;264(19):11158–11167. [PubMed] [Google Scholar]
  25. Schaeffer A. J., Amundsen S. K., Schmidt L. N. Adherence of Escherichia coli to human urinary tract epithelial cells. Infect Immun. 1979 Jun;24(3):753–759. doi: 10.1128/iai.24.3.753-759.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Schaeffer A. J., Jones J. M., Dunn J. K. Association of vitro Escherichia coli adherence to vaginal and buccal epithelial cells with susceptibility of women to recurrent urinary-tract infections. N Engl J Med. 1981 Apr 30;304(18):1062–1066. doi: 10.1056/NEJM198104303041802. [DOI] [PubMed] [Google Scholar]
  27. Schwan W. R., Seifert H. S., Duncan J. L. Growth conditions mediate differential transcription of fim genes involved in phase variation of type 1 pili. J Bacteriol. 1992 Apr;174(7):2367–2375. doi: 10.1128/jb.174.7.2367-2375.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sheinfeld J., Schaeffer A. J., Cordon-Cardo C., Rogatko A., Fair W. R. Association of the Lewis blood-group phenotype with recurrent urinary tract infections in women. N Engl J Med. 1989 Mar 23;320(12):773–777. doi: 10.1056/NEJM198903233201205. [DOI] [PubMed] [Google Scholar]
  29. Stapleton A., Nudelman E., Clausen H., Hakomori S., Stamm W. E. Binding of uropathogenic Escherichia coli R45 to glycolipids extracted from vaginal epithelial cells is dependent on histo-blood group secretor status. J Clin Invest. 1992 Sep;90(3):965–972. doi: 10.1172/JCI115973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Watkins W. M. Biochemistry and Genetics of the ABO, Lewis, and P blood group systems. Adv Hum Genet. 1980;10:1-136, 379-85. doi: 10.1007/978-1-4615-8288-5_1. [DOI] [PubMed] [Google Scholar]
  31. van Die I., Hoekstra W., Bergmans H. Analysis of the primary structure of P-fimbrillins of uropathogenic Escherichia coli. Microb Pathog. 1987 Sep;3(3):149–154. doi: 10.1016/0882-4010(87)90091-x. [DOI] [PubMed] [Google Scholar]
  32. van't Wout J., Burnette W. N., Mar V. L., Rozdzinski E., Wright S. D., Tuomanen E. I. Role of carbohydrate recognition domains of pertussis toxin in adherence of Bordetella pertussis to human macrophages. Infect Immun. 1992 Aug;60(8):3303–3308. doi: 10.1128/iai.60.8.3303-3308.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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