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. 1994 Aug;62(8):3543–3546. doi: 10.1128/iai.62.8.3543-3546.1994

Altered expression of surface alpha-1,3-glucan in genetically related strains of Blastomyces dermatitidis that differ in virulence.

L H Hogan 1, B S Klein 1
PMCID: PMC302989  PMID: 8039925

Abstract

Recent studies of the dimorphic fungal pathogens Histoplasma capsulatum and Paracoccidioides brasiliensis have suggested a role in virulence for the cell surface carbohydrate alpha-(1,3)-glucan. To investigate a possible basis for alpha-(1,3)-glucan in the pathogenicity and virulence of the dimorphic fungus Blastomyces dermatitidis, we examined three genetically related strains of B. dermatitidis that differ in their virulence for mice: wild-type virulent strain ATCC 26199; mutant strain ATCC 60915, which is 10,000-fold reduced in virulence; and mutant strain ATCC 60916, which is avirulent. Immunologic quantitation of cell wall alpha-(1,3)-glucan revealed that the mutant yeasts were almost devoid of this sugar moiety, in contrast to the high concentration of alpha-(1,3)-glucan on the cell wall of the wild-type yeasts. These differences are discussed in relation to previous studies of yeast surface expression of the WI-1 antigen and recognition and binding of the related strains by human monocyte-derived macrophages.

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Selected References

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  1. Brass C., Volkmann C. M., Philpott D. E., Klein H. P., Halde C. J., Stevens D. A. Spontaneous mutant of Blastomyces dermatitidis attenuated in virulence for mice. Sabouraudia. 1982 Jun;20(2):145–158. [PubMed] [Google Scholar]
  2. Cox R. A., Best G. K. Cell wall composition of two strains of Blastomyces dermatitidis exhibiting differences in virulence for mice. Infect Immun. 1972 Apr;5(4):449–453. doi: 10.1128/iai.5.4.449-453.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DENTON J. F., DISALVO A. F. ISOLATION OF BLASTOMYCES DERMATITIDIS FROM NATURAL SITES AT AUGUSTA, GEORGIA. Am J Trop Med Hyg. 1964 Sep;13:716–722. doi: 10.4269/ajtmh.1964.13.716. [DOI] [PubMed] [Google Scholar]
  4. DENTON J. F., McDONOUGH E. S., AJELLO L., AUSHERMAN R. J. Isolation of Blastomyces dermatitidis from soil. Science. 1961 Apr 14;133(3459):1126–1127. doi: 10.1126/science.133.3459.1126. [DOI] [PubMed] [Google Scholar]
  5. DISALVO A. F., DENTON J. F. LIPID CONTENT OF FOUR STRAINS OF BLASTOMYCES DERMATITIDIS OF DIFFERENT MOUSE VIRULENCE. J Bacteriol. 1963 Apr;85:927–931. doi: 10.1128/jb.85.4.927-931.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drutz D. J., Frey C. L. Intracellular and extracellular defenses of human phagocytes against Blastomyces dermatitidis conidia and yeasts. J Lab Clin Med. 1985 Jun;105(6):737–750. [PubMed] [Google Scholar]
  7. Eissenberg L. G., Goldman W. E. Histoplasma variation and adaptive strategies for parasitism: new perspectives on histoplasmosis. Clin Microbiol Rev. 1991 Oct;4(4):411–421. doi: 10.1128/cmr.4.4.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harvey R. P., Schmid E. S., Carrington C. C., Stevens D. A. Mouse model of pulmonary blastomycosis: utility, simplicity, and quantitative parameters. Am Rev Respir Dis. 1978 Apr;117(4):695–703. doi: 10.1164/arrd.1978.117.4.695. [DOI] [PubMed] [Google Scholar]
  9. Klein B. S., Vergeront J. M., Weeks R. J., Kumar U. N., Mathai G., Varkey B., Kaufman L., Bradsher R. W., Stoebig J. F., Davis J. P. Isolation of Blastomyces dermatitidis in soil associated with a large outbreak of blastomycosis in Wisconsin. N Engl J Med. 1986 Feb 27;314(9):529–534. doi: 10.1056/NEJM198602273140901. [DOI] [PubMed] [Google Scholar]
  10. Klimpel K. R., Goldman W. E. Cell walls from avirulent variants of Histoplasma capsulatum lack alpha-(1,3)-glucan. Infect Immun. 1988 Nov;56(11):2997–3000. doi: 10.1128/iai.56.11.2997-3000.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klimpel K. R., Goldman W. E. Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum. Infect Immun. 1987 Mar;55(3):528–533. doi: 10.1128/iai.55.3.528-533.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morrison C. J., Stevens D. A. Mechanisms of fungal pathogenicity: correlation of virulence in vivo, susceptibility to killing by polymorphonuclear neutrophils in vitro, and neutrophil superoxide anion induction among Blastomyces dermatitidis isolates. Infect Immun. 1991 Aug;59(8):2744–2749. doi: 10.1128/iai.59.8.2744-2749.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. San-Blas F., San-Blas G., Cova L. J. A morphological mutant of Paracoccidioides brasiliensis strain IVIC Pb9. Isolation and wall characterization. J Gen Microbiol. 1976 Apr;93(2):209–218. doi: 10.1099/00221287-93-2-209. [DOI] [PubMed] [Google Scholar]
  14. San-Blas G., Vernet D. Induction of the synthesis of cell wall alpha-1,3-glucan in the yeastlike form of Paracoccidioides brasiliensis strain IVIC Pb9 by fetal calf serum. Infect Immun. 1977 Mar;15(3):897–902. doi: 10.1128/iai.15.3.897-902.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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