Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1994 Aug;62(8):3590–3593. doi: 10.1128/iai.62.8.3590-3593.1994

Structure-function relationships for polysaccharide-induced intra-abdominal abscesses.

A O Tzianabos 1, A B Onderdonk 1, R S Smith 1, D L Kasper 1
PMCID: PMC303001  PMID: 8039936

Abstract

We have previously shown that in an animal model of sepsis positively and negatively charged groups on polysaccharide A of Bacteroides fragilis are essential for the induction of intra-abdominal abscess formation (A. O. Tzianabos, A. B. Onderdonk, B. Rosner, R. L. Cisneros, and D. L. Kasper, Science 262:416-419, 1993). By introducing chemical modifications into the structures of B. fragilis polysaccharide B as well as other abscess-inducing bacterial polysaccharides, we observed the following. (i) The presence of a nonacetylated free amino group on these polysaccharides appears to be required for abscess induction. (ii) No specific type of negatively charged group is essential to abscess induction by these polysaccharides. (iii) The density of free amino groups on these polysaccharides influences this pathobiologic host response.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann H., Tzianabos A. O., Brisson J. R., Kasper D. L., Jennings H. J. Structural elucidation of two capsular polysaccharides from one strain of Bacteroides fragilis using high-resolution NMR spectroscopy. Biochemistry. 1992 Apr 28;31(16):4081–4089. doi: 10.1021/bi00131a026. [DOI] [PubMed] [Google Scholar]
  2. Bhattacharjee A. K., Jennings H. J., Kenny C. P., Martin A., Smith I. C. Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem. 1975 Mar 10;250(5):1926–1932. [PubMed] [Google Scholar]
  3. Jennings H. J., Katzenellenbogen E., Lugowski C., Kasper D. L. Structure of native polysaccharide antigens of type Ia and type Ib group B Streptococcus. Biochemistry. 1983 Mar 1;22(5):1258–1264. doi: 10.1021/bi00274a042. [DOI] [PubMed] [Google Scholar]
  4. Jennings H. J., Lugowski C., Young N. M. Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1. Biochemistry. 1980 Sep 30;19(20):4712–4719. doi: 10.1021/bi00561a026. [DOI] [PubMed] [Google Scholar]
  5. Kasper D. L., Onderdonk A. B., Crabb J., Bartlett J. G. Protective efficacy of immunization with capsular antigen against experimental infection with Bacteroides fragilis. J Infect Dis. 1979 Nov;140(5):724–731. doi: 10.1093/infdis/140.5.724. [DOI] [PubMed] [Google Scholar]
  6. Lindberg B., Lindqvist B., Lönngren J., Powell D. A. Structural studies of the capsular polysaccharide from Streptococcus pneumoniae type 1. Carbohydr Res. 1980 Jan 1;78(1):111–117. doi: 10.1016/s0008-6215(00)83664-2. [DOI] [PubMed] [Google Scholar]
  7. Lindberg B., Lönngren J., Powell D. A. Structural studies on the specific type-14 pneumococcal polysaccharide. Carbohydr Res. 1977 Sep;58(1):177–186. doi: 10.1016/s0008-6215(00)83413-8. [DOI] [PubMed] [Google Scholar]
  8. Onderdonk A. B., Kasper D. L., Cisneros R. L., Bartlett J. G. The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. J Infect Dis. 1977 Jul;136(1):82–89. doi: 10.1093/infdis/136.1.82. [DOI] [PubMed] [Google Scholar]
  9. Pantosti A., Tzianabos A. O., Onderdonk A. B., Kasper D. L. Immunochemical characterization of two surface polysaccharides of Bacteroides fragilis. Infect Immun. 1991 Jun;59(6):2075–2082. doi: 10.1128/iai.59.6.2075-2082.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Polk B. F., Kasper D. L. Bacteroides fragilis subspecies in clinical isolates. Ann Intern Med. 1977 May;86(5):569–571. doi: 10.7326/0003-4819-86-5-569. [DOI] [PubMed] [Google Scholar]
  11. Rhodes J. Erythrocyte rosettes provide an analogue for Schiff base formation in specific T cell activation. J Immunol. 1990 Jul 15;145(2):463–469. [PubMed] [Google Scholar]
  12. Rhodes J. Evidence for an intercellular covalent reaction essential in antigen-specific T cell activation. J Immunol. 1989 Sep 1;143(5):1482–1489. [PubMed] [Google Scholar]
  13. Shapiro M. E., Kasper D. L., Zaleznik D. F., Spriggs S., Onderdonk A. B., Finberg R. W. Cellular control of abscess formation: role of T cells in the regulation of abscesses formed in response to Bacteroides fragilis. J Immunol. 1986 Jul 1;137(1):341–346. [PubMed] [Google Scholar]
  14. Tzianabos A. O., Onderdonk A. B., Rosner B., Cisneros R. L., Kasper D. L. Structural features of polysaccharides that induce intra-abdominal abscesses. Science. 1993 Oct 15;262(5132):416–419. doi: 10.1126/science.8211161. [DOI] [PubMed] [Google Scholar]
  15. Tzianabos A. O., Pantosti A., Baumann H., Brisson J. R., Jennings H. J., Kasper D. L. The capsular polysaccharide of Bacteroides fragilis comprises two ionically linked polysaccharides. J Biol Chem. 1992 Sep 5;267(25):18230–18235. [PubMed] [Google Scholar]
  16. Wessels M. R., Pozsgay V., Kasper D. L., Jennings H. J. Structure and immunochemistry of an oligosaccharide repeating unit of the capsular polysaccharide of type III group B Streptococcus. A revised structure for the type III group B streptococcal polysaccharide antigen. J Biol Chem. 1987 Jun 15;262(17):8262–8267. [PubMed] [Google Scholar]
  17. Zheng B., Brett S. J., Tite J. P., Lifely M. R., Brodie T. A., Rhodes J. Galactose oxidation in the design of immunogenic vaccines. Science. 1992 Jun 12;256(5063):1560–1563. doi: 10.1126/science.1598588. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES