Figure 5.—

Disruption of N signaling does not alter Hh signaling. Ci (magenta) and Ptc (red) expression in third instar wing discs (A–L) and pupal wings (M–P). Clones are negatively marked by lack of GFP or lacZ expression (green). (A and B) w⁻ wing discs. (C and D) Wing discs from N^l1N-ts1 larvae shifted to 30° (restrictive temperature) for 24 hr. (E–H) Clone of null allele N^55e11 within the AP organizer. (I–L) Ventral clone in the posterior compartment and a dorsal clone in the anterior compartment of null allele Su(H)^del47. (H and L) Merged images showing the positions of these clones in relation to Ptc and Ci expression. (M–P) Expression of Ptc and Ci in w⁻ and temperature-shifted N^ts pupal wings. (M and N) w⁻ pupal wings. Note that at this stage dorsal and ventral surfaces of the wing are not apposed to each other, resulting in the apparent splitting of the Pct and Ci stripes in the proximal region (left) of the wing. (O and P) N^l1N-ts1 pupal wings shifted to 30° for 24 hr.