Figure 5.

SDS-PAGE/Western blot comparison of the ability of detergents and cholesterol analogs to extract a His₁₀-tagged GPCR, hKOR1, from bacterial membranes. The following test concentrations were used: 2% CHAPSO, 1.5% Digitonin, 2% CHOBIMALT, 2% DDM, 1% LMPC, and 1% DPC. Samples loaded onto this gel were extracts from membrane aliquots, each containing 15 μg of protein. Western blot detection was based on use of a mouse Penta-His primary antibody and mouse IgG-alkaline phosphatase secondary antibody followed by color development mediated by alkaline phosphatase. Positions of the monomer and dimer receptor bands are indicated by the (m) and (d) on the left side.