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. 1994 Oct;62(10):4664–4666. doi: 10.1128/iai.62.10.4664-4666.1994

Serum Cohn fraction IV-1 supports the growth of Giardia lamblia in vitro.

H D Lujan 1, L G Byrd 1, M R Mowatt 1, T E Nash 1
PMCID: PMC303162  PMID: 7927737

Abstract

Bovine serum Cohn fractions were substituted for whole bovine serum in TYI-S-33 medium. Only fraction IV-1 supported attachment, cysteine uptake, and growth of Giardia lamblia. Among the mammalian sources tested, only goat and horse fractions showed activities comparable to that of the bovine fraction. Agglutinating immunoglobulins were identified as deleterious agents in human, rat, rabbit, and dog fractions.

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Selected References

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  1. Adam R. D. The biology of Giardia spp. Microbiol Rev. 1991 Dec;55(4):706–732. doi: 10.1128/mr.55.4.706-732.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bifulco J. M., Schaefer F. W., 3rd Serial subcultivation of Giardia lamblia in Keister's modified TYI-S-33 medium containing ultroser G. J Protozool. 1992 Jan-Feb;39(1):211–213. doi: 10.1111/j.1550-7408.1992.tb01304.x. [DOI] [PubMed] [Google Scholar]
  3. Blum W. F., Ranke M. B., Bierich J. R. Isolation and partial characterization of six somatomedin-like peptides from human plasma Cohn fraction IV. Acta Endocrinol (Copenh) 1986 Feb;111(2):271–284. doi: 10.1530/acta.0.1110271. [DOI] [PubMed] [Google Scholar]
  4. Bruderer T., Papanastasiou P., Castro R., Köhler P. Variant cysteine-rich surface proteins of Giardia isolates from human and animal sources. Infect Immun. 1993 Jul;61(7):2937–2944. doi: 10.1128/iai.61.7.2937-2944.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cham B. E., Knowles B. R. A solvent system for delipidation of plasma or serum without protein precipitation. J Lipid Res. 1976 Mar;17(2):176–181. [PubMed] [Google Scholar]
  6. Congote L. F., Esch F. The major erythrotropin of bovine Cohn fraction V has the N-terminal sequence of insulin-like growth factor II with isoleucine at position 35. Biochem Biophys Res Commun. 1987 Dec 31;149(3):1026–1032. doi: 10.1016/0006-291x(87)90511-0. [DOI] [PubMed] [Google Scholar]
  7. Congote L. F. Extraction of an erythrotropin-like factor from bovine serum albumin (Cohn fraction V). In Vitro Cell Dev Biol. 1987 May;23(5):361–366. doi: 10.1007/BF02620993. [DOI] [PubMed] [Google Scholar]
  8. Farthing M. J., Varon S. R., Keusch G. T. Mammalian bile promotes growth of Giardia lamblia in axenic culture. Trans R Soc Trop Med Hyg. 1983;77(4):467–469. doi: 10.1016/0035-9203(83)90115-3. [DOI] [PubMed] [Google Scholar]
  9. Gillin F. D., Gault M. J., Hofmann A. F., Gurantz D., Sauch J. F. Biliary lipids support serum-free growth of Giardia lamblia. Infect Immun. 1986 Sep;53(3):641–645. doi: 10.1128/iai.53.3.641-645.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gillin F. D., Reiner D. S. Attachment of the flagellate Giardia lamblia: role of reducing agents, serum, temperature, and ionic composition. Mol Cell Biol. 1982 Apr;2(4):369–377. doi: 10.1128/mcb.2.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gold D., Ofek I. Adhesion of Trichomonas vaginalis to plastic surfaces: requirement for energy and serum constituents. Parasitology. 1992 Aug;105(Pt 1):55–62. doi: 10.1017/s0031182000073686. [DOI] [PubMed] [Google Scholar]
  12. Guy R. A., Bertrand S., Faubert G. M. Modification of RPMI 1640 for use in vitro immunological studies of host-parasite interactions in giardiasis. J Clin Microbiol. 1991 Mar;29(3):627–629. doi: 10.1128/jcm.29.3.627-629.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jarroll E. L., Muller P. J., Meyer E. A., Morse S. A. Lipid and carbohydrate metabolism of Giardia lamblia. Mol Biochem Parasitol. 1981 Feb;2(3-4):187–196. doi: 10.1016/0166-6851(81)90099-2. [DOI] [PubMed] [Google Scholar]
  14. Keister D. B. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg. 1983;77(4):487–488. doi: 10.1016/0035-9203(83)90120-7. [DOI] [PubMed] [Google Scholar]
  15. Lujan H. D., Nash T. E. The uptake and metabolism of cysteine by Giardia lamblia trophozoites. J Eukaryot Microbiol. 1994 Mar-Apr;41(2):169–175. doi: 10.1111/j.1550-7408.1994.tb01491.x. [DOI] [PubMed] [Google Scholar]
  16. Luján H. D., Mowatt M. R., Helman L. J., Nash T. E. Insulin-like growth factors stimulate growth and L-cysteine uptake by the intestinal parasite Giardia lamblia. J Biol Chem. 1994 May 6;269(18):13069–13072. [PubMed] [Google Scholar]
  17. Meloni B. P., Thompson R. C. Comparative studies on the axenic in vitro cultivation of Giardia of human and canine origin: evidence for intraspecific variation. Trans R Soc Trop Med Hyg. 1987;81(4):637–640. doi: 10.1016/0035-9203(87)90438-x. [DOI] [PubMed] [Google Scholar]
  18. Meyer E. A. Isolation and axenic cultivation of Giardia trophozoites from the rabbit, chinchilla, and cat. Exp Parasitol. 1970 Apr;27(2):179–183. doi: 10.1016/0014-4894(70)90023-8. [DOI] [PubMed] [Google Scholar]
  19. Mowatt M. R., Aggarwal A., Nash T. E. Carboxy-terminal sequence conservation among variant-specific surface proteins of Giardia lamblia. Mol Biochem Parasitol. 1991 Dec;49(2):215–227. doi: 10.1016/0166-6851(91)90065-e. [DOI] [PubMed] [Google Scholar]
  20. Nash T. E., Aggarwal A., Adam R. D., Conrad J. T., Merritt J. W., Jr Antigenic variation in Giardia lamblia. J Immunol. 1988 Jul 15;141(2):636–641. [PubMed] [Google Scholar]
  21. Nash T. E., Aggarwal A. Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates. J Immunol. 1986 Apr 1;136(7):2628–2632. [PubMed] [Google Scholar]
  22. Nash T. Surface antigen variability and variation in Giardia lamblia. Parasitol Today. 1992 Jul;8(7):229–234. doi: 10.1016/0169-4758(92)90119-m. [DOI] [PubMed] [Google Scholar]
  23. Straczek J., Heulin M. H., Chenut A. M., Lasbennes A., Geschier C., Belleville F., Nabet P. Purification and characterization of three molecular forms of insulin-like growth factor II from human Cohn paste IV. J Chromatogr. 1990 Nov 16;532(2):237–248. doi: 10.1016/s0378-4347(00)83775-5. [DOI] [PubMed] [Google Scholar]
  24. Strandén A. M., Köhler P. Swiss Giardia isolates of different host origin show great similarities in their metabolism. Parasitol Res. 1991;77(5):455–457. doi: 10.1007/BF00931645. [DOI] [PubMed] [Google Scholar]
  25. Thompson R. C., Reynoldson J. A., Mendis A. H. Giardia and giardiasis. Adv Parasitol. 1993;32:71–160. doi: 10.1016/s0065-308x(08)60207-9. [DOI] [PubMed] [Google Scholar]
  26. Van den Brande J. L., Hoogerbrugge C. M., Beyreuther K., Roepstorff P., Jansen J., van Buul-Offers S. C. Isolation and partial characterization of IGF-like peptides from Cohn fraction IV of human plasma. Acta Endocrinol (Copenh) 1990 Jun;122(6):683–695. doi: 10.1530/acta.0.1220683. [DOI] [PubMed] [Google Scholar]
  27. Wieder S. C., Keister D. B., Reiner D. S. Mass cultivation of Giardia lamblia in a serum-free medium. J Parasitol. 1983 Dec;69(6):1181–1182. [PubMed] [Google Scholar]

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