Figure 4.

Definitive hematopoiesis is severely impaired in meis1 MO-injected embryos. (A–B) At 72 hpf no cd41-positive cells were detectable in meis1 MO-injected Tg(cd41:EGFP) transgenic embryos in comparison with the control. (C–D) C-myb is normally expressed in the ICM of control MO-injected embryos (C) at 24 hpf and its expression was not affected by the loss of meis1 (D). (E–F) At 30 hpf in control embryos (E) c-myb expression was mostly confined to the AGM (arrow in white dotted inset) in contrast to meis1 MO-injected embryos (F) where c-myb was expressed throughout the aortic roof and vein (right square bracket in white dotted inset).