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Infection and Immunity logoLink to Infection and Immunity
. 1994 Nov;62(11):5173–5176. doi: 10.1128/iai.62.11.5173-5176.1994

Arginine-derived nitric oxide reduces fecal oocyst shedding in nude mice infected with Cryptosporidium parvum.

G J Leitch 1, Q He 1
PMCID: PMC303243  PMID: 7523306

Abstract

Dietary L-arginine (4%) significantly reduced fecal oocyst shedding in athymic nude mice chronically infected with Cryptosporidium parvum. This effect appeared to be due to an increase in host nitric oxide (NO) production as it was not observed in arginine-supplemented animals administered the NO synthase inhibitor, N-nitro-L-arginine methyl ester. N-Nitro-L-arginine methyl ester alone significantly increased fecal oocyst shedding in chronically infected animals. In in vitro assays, oocyst excystation and sporozoite viability were significantly reduced by the NO donors sodium nitroprusside and S-nitroso-L-acetyl penicillamine in a concentration-dependent manner. These data suggest that arginine-derived NO may reduce the parasite load in experimental cryptosporidiosis.

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Selected References

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  1. Adams L. B., Hibbs J. B., Jr, Taintor R. R., Krahenbuhl J. L. Microbiostatic effect of murine-activated macrophages for Toxoplasma gondii. Role for synthesis of inorganic nitrogen oxides from L-arginine. J Immunol. 1990 Apr 1;144(7):2725–2729. [PubMed] [Google Scholar]
  2. Alspaugh J. A., Granger D. L. Inhibition of Cryptococcus neoformans replication by nitrogen oxides supports the role of these molecules as effectors of macrophage-mediated cytostasis. Infect Immun. 1991 Jul;59(7):2291–2296. doi: 10.1128/iai.59.7.2291-2296.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arrowood M. J., Jaynes J. M., Healey M. C. In vitro activities of lytic peptides against the sporozoites of Cryptosporidium parvum. Antimicrob Agents Chemother. 1991 Feb;35(2):224–227. doi: 10.1128/aac.35.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boockvar K. S., Granger D. L., Poston R. M., Maybodi M., Washington M. K., Hibbs J. B., Jr, Kurlander R. L. Nitric oxide produced during murine listeriosis is protective. Infect Immun. 1994 Mar;62(3):1089–1100. doi: 10.1128/iai.62.3.1089-1100.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Croen K. D. Evidence for antiviral effect of nitric oxide. Inhibition of herpes simplex virus type 1 replication. J Clin Invest. 1993 Jun;91(6):2446–2452. doi: 10.1172/JCI116479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Current W. L., Garcia L. S. Cryptosporidiosis. Clin Lab Med. 1991 Dec;11(4):873–897. [PubMed] [Google Scholar]
  7. Di Rosa M., Radomski M., Carnuccio R., Moncada S. Glucocorticoids inhibit the induction of nitric oxide synthase in macrophages. Biochem Biophys Res Commun. 1990 Nov 15;172(3):1246–1252. doi: 10.1016/0006-291x(90)91583-e. [DOI] [PubMed] [Google Scholar]
  8. Doi T., Ando M., Akaike T., Suga M., Sato K., Maeda H. Resistance to nitric oxide in Mycobacterium avium complex and its implication in pathogenesis. Infect Immun. 1993 May;61(5):1980–1989. doi: 10.1128/iai.61.5.1980-1989.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gianotti L., Alexander J. W., Pyles T., Fukushima R. Arginine-supplemented diets improve survival in gut-derived sepsis and peritonitis by modulating bacterial clearance. The role of nitric oxide. Ann Surg. 1993 Jun;217(6):644–654. doi: 10.1097/00000658-199306000-00006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heine J., Moon H. W., Woodmansee D. B. Persistent Cryptosporidium infection in congenitally athymic (nude) mice. Infect Immun. 1984 Mar;43(3):856–859. doi: 10.1128/iai.43.3.856-859.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kirk S. J., Regan M. C., Wasserkrug H. L., Sodeyama M., Barbul A. Arginine enhances T-cell responses in athymic nude mice. JPEN J Parenter Enteral Nutr. 1992 Sep-Oct;16(5):429–432. doi: 10.1177/0148607192016005429. [DOI] [PubMed] [Google Scholar]
  12. Kröncke K. D., Brenner H. H., Rodriguez M. L., Etzkorn K., Noack E. A., Kolb H., Kolb-Bachofen V. Pancreatic islet cells are highly susceptible towards the cytotoxic effects of chemically generated nitric oxide. Biochim Biophys Acta. 1993 Sep 8;1182(2):221–229. doi: 10.1016/0925-4439(93)90144-p. [DOI] [PubMed] [Google Scholar]
  13. Leitch G. J., He Q. Putative anticryptosporidial agents tested with an immunodeficient mouse model. Antimicrob Agents Chemother. 1994 Apr;38(4):865–867. doi: 10.1128/aac.38.4.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lemeteil D., Roussel F., Favennec L., Ballet J. J., Brasseur P. Assessment of candidate anticryptosporidial agents in an immunosuppressed rat model. J Infect Dis. 1993 Mar;167(3):766–768. doi: 10.1093/infdis/167.3.766. [DOI] [PubMed] [Google Scholar]
  15. Liew F. Y., Li Y., Millott S. Tumour necrosis factor (TNF-alpha) in leishmaniasis. II. TNF-alpha-induced macrophage leishmanicidal activity is mediated by nitric oxide from L-arginine. Immunology. 1990 Dec;71(4):556–559. [PMC free article] [PubMed] [Google Scholar]
  16. McGowan I., Hawkins A. S., Weller I. V. The natural history of cryptosporidial diarrhoea in HIV-infected patients. AIDS. 1993 Mar;7(3):349–354. doi: 10.1097/00002030-199303000-00007. [DOI] [PubMed] [Google Scholar]
  17. Mead J. R., Arrowood M. J., Sidwell R. W., Healey M. C. Chronic Cryptosporidium parvum infections in congenitally immunodeficient SCID and nude mice. J Infect Dis. 1991 Jun;163(6):1297–1304. doi: 10.1093/infdis/163.6.1297. [DOI] [PubMed] [Google Scholar]
  18. Moncada S., Palmer R. M., Higgs E. A. Biosynthesis of nitric oxide from L-arginine. A pathway for the regulation of cell function and communication. Biochem Pharmacol. 1989 Jun 1;38(11):1709–1715. doi: 10.1016/0006-2952(89)90403-6. [DOI] [PubMed] [Google Scholar]
  19. Rockett K. A., Awburn M. M., Cowden W. B., Clark I. A. Killing of Plasmodium falciparum in vitro by nitric oxide derivatives. Infect Immun. 1991 Sep;59(9):3280–3283. doi: 10.1128/iai.59.9.3280-3283.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shaffer J. E., Han B. J., Chern W. H., Lee F. W. Lack of tolerance to a 24-hour infusion of S-nitroso N-acetylpenicillamine (SNAP) in conscious rabbits. J Pharmacol Exp Ther. 1992 Jan;260(1):286–293. [PubMed] [Google Scholar]
  21. Ungar B. L., Kao T. C., Burris J. A., Finkelman F. D. Cryptosporidium infection in an adult mouse model. Independent roles for IFN-gamma and CD4+ T lymphocytes in protective immunity. J Immunol. 1991 Aug 1;147(3):1014–1022. [PubMed] [Google Scholar]
  22. Vincendeau P., Daulouède S. Macrophage cytostatic effect on Trypanosoma musculi involves an L-arginine-dependent mechanism. J Immunol. 1991 Jun 15;146(12):4338–4343. [PubMed] [Google Scholar]
  23. Vincendeau P., Daulouède S., Veyret B., Darde M. L., Bouteille B., Lemesre J. L. Nitric oxide-mediated cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei. Exp Parasitol. 1992 Nov;75(3):353–360. doi: 10.1016/0014-4894(92)90220-5. [DOI] [PubMed] [Google Scholar]
  24. Zhu L., Gunn C., Beckman J. S. Bactericidal activity of peroxynitrite. Arch Biochem Biophys. 1992 Nov 1;298(2):452–457. doi: 10.1016/0003-9861(92)90434-x. [DOI] [PubMed] [Google Scholar]

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