Fig. 1.

Acquisition and culture of human prostate specimens. A, Flow chart and schematic of sample acquisition. Posterior view of prostate with attached seminal vesicles showing removal of a complete cross-section. Open circles in cross-section indicate hypothetical locations of plug removal after inspection of toluidine staining under a high-power dissecting scope. Arrows from circles indicate the flow of plugs for tissue culture. Arrow below indicates flow of the remainder of prostate specimen. Diagonal arrow indicates the flow of the longitudinal slice for pathological evaluation. TZ, Transition zone. B, Schematic of processing of samples removed for growth of epithelial and/or stromal cells as described above.