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. 2010 Aug 12;117(3):1081–1090. doi: 10.1182/blood-2010-02-267427

Figure 2.

Figure 2

VEGF-A–concentration dependent corneal angiogenesis and lymphangiogenesis. Control pellets (PBS) or pellets with varying amounts of VEGF-A (25, 100, 400, and 1600 ng) were implanted into the corneas of C57BL/6 mice. Corneal angiogenesis and lymphangiogenesis were examined on day 6 after pellet implantation. (A) Double staining of corneal flat mounts for angiogenesis (CD31, green) and lymphangiogenesis (LYVE-1, red) in the front, side, and the back of the cornea. *Implanted pellets. Bar, 200 μm. (B) Quantitative analysis of angiogenesis and lymphangiogenesis on day 6 (n = 5-8). (C) Quantitation of the number of LYVE-1(+) lymphatic vessels in corneas of PBS or VEGF-A– (25, 100, 400, or 1600 ng) implanted animals (n = 5-6). (D) Ratio of the distance of lymphatic tips and vascular tips from limbal vessels in corneas of PBS or VEGF-A– (25, 100, 400, or 1600 ng) implanted animals (n = 15-22). (E) Quantitation of the number of lymphatic vessels (n = 4-10). Bar, 200 μm. *P < .05; **P < .01.