Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1988 Jul;82(1):350–353. doi: 10.1172/JCI113593

The order of islet microvascular cellular perfusion is B----A----D in the perfused rat pancreas.

E Samols 1, J I Stagner 1, R B Ewart 1, V Marks 1
PMCID: PMC303515  PMID: 2455737

Abstract

In order to determine whether microvascular blood flow is important in the regulation of intra-islet cellular interactions, rat pancreata were isolated and perfused in vitro, both anterogradely or retrogradely, with and without anti-insulin or anti-somatostatin gamma-globulin. Expressed as percent change, anterograde infusion of insulin antibody increased efflux concentrations of glucagon (110 +/- 20%, P less than 0.0005) and somatostatin (2,112 +/- 73%, P less than 0.0005) above their respective control. Retrograde infusion of insulin antibody did not affect efflux concentrations of glucagon (P less than 0.50) or somatostatin (P less than 0.50). The anterograde infusion of anti-somatostatin antibody had no effect upon insulin (P less than 0.50) or glucagon (P less than 0.50) efflux concentrations, whereas retrograde anti-somatostatin antibody infusion produced immediate increases in efflux concentrations of both insulin (115 +/- 33%, P less than 0.0005) and glucagon (77 +/- 8%, P less than 0.0005). These results strongly suggest that (a) the vascular compartment is important in the regulation of intra-islet cellular interactions and further suggest that (b) the order of islet cellular perfusion and interaction is from the B cell core outward to the mantle, and (c) the mantle is further subordered with the majority of D cells downstream or distal to the majority of A cells. Thus, in the vascular compartment, B cells inhibit A-cell secretion and A cells stimulate D-cell secretion.

Full text

PDF
350

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner-Weir S., Orci L. New perspectives on the microvasculature of the islets of Langerhans in the rat. Diabetes. 1982 Oct;31(10):883–889. doi: 10.2337/diab.31.10.883. [DOI] [PubMed] [Google Scholar]
  2. Burdette S., Schwartz R. S. Current concepts: immunology. Idiotypes and idiotypic networks. N Engl J Med. 1987 Jul 23;317(4):219–224. doi: 10.1056/NEJM198707233170407. [DOI] [PubMed] [Google Scholar]
  3. Filipponi P., Gregorio F., Cristallini S., Ferrandina C., Nicoletti I., Santeusanio F. Selective impairment of pancreatic A cell suppression by glucose during acute alloxan-induced insulinopenia: in vitro study on isolated perfused rat pancreas. Endocrinology. 1986 Jul;119(1):408–415. doi: 10.1210/endo-119-1-408. [DOI] [PubMed] [Google Scholar]
  4. Grodsky G. M., Fanska R. E. The in vitro perfused pancreas. Methods Enzymol. 1975;39:364–372. doi: 10.1016/s0076-6879(75)39033-2. [DOI] [PubMed] [Google Scholar]
  5. Kawai K., Chiba Y., Okuda Y., Yamashita K. Hormone release from pancreatic islets perfused from venous side. Diabetes. 1987 Mar;36(3):256–260. doi: 10.2337/diab.36.3.256. [DOI] [PubMed] [Google Scholar]
  6. Maruyama H., Hisatomi A., Orci L., Grodsky G. M., Unger R. H. Insulin within islets is a physiologic glucagon release inhibitor. J Clin Invest. 1984 Dec;74(6):2296–2299. doi: 10.1172/JCI111658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Orci L., Malaisse-Lagae F., Ravazzola M., Rouiller D., Renold A. E., Perrelet A., Unger R. A morphological basis for intercellular communication between alpha- and beta-cells in the endocrine pancreas. J Clin Invest. 1975 Oct;56(4):1066–1070. doi: 10.1172/JCI108154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orci L., Unger R. H. Functional subdivision of islets of Langerhans and possible role of D cells. Lancet. 1975 Dec 20;2(7947):1243–1244. doi: 10.1016/s0140-6736(75)92078-4. [DOI] [PubMed] [Google Scholar]
  9. Reilly T. M., Root R. T. Production of idiotypic and anti-idiotypic antibodies by BALB/c mice in response to immunizations with glucagon, vasopressin, or insulin: supporting evidence for the network concept. J Immunol. 1986 Jul 15;137(2):597–602. [PubMed] [Google Scholar]
  10. Stagner J. I., Samols E. Retrograde perfusion as a model for testing the relative effects of glucose versus insulin on the A cell. J Clin Invest. 1986 Mar;77(3):1034–1037. doi: 10.1172/JCI112356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tan K., Atabani G., Marks V. Divergent effect of glucagon antibodies on arginine and glucose-stimulated insulin secretion in the rat. Diabetologia. 1985 Jul;28(7):441–444. doi: 10.1007/BF00280888. [DOI] [PubMed] [Google Scholar]
  12. Weir G. C., Samols E., Loo S., Patel Y. C., Gabbay K. H. Somatostatin and pancreatic polypeptide secretion: effects of glucagon, insulin, and arginine. Diabetes. 1979 Jan;28(1):35–40. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES