Skip to main content
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1988 Aug;82(2):508–513. doi: 10.1172/JCI113625

Desialation of transferrin by rat liver endothelium.

S Irie 1, T Kishimoto 1, M Tavassoli 1
PMCID: PMC303541  PMID: 3165384

Abstract

To examine the role of liver endothelium in desialation of transferrin (TF), pulse-chase studies were done by incubation of either 3H (sialic acid labeled)-, or 125I, or 59Fe (protein core labeled)-TF with fractionated liver endothelium. While 125I or 59Fe labels were externalized after initial binding and internalization, a large proportion of 3H label was internalized and remained within the cell. When the supernatant of these experiments was studied by isoelectricfocusing and Ricinus communis agglutinin (RCA120) affinity chromatography, generation of asialotransferrin was noted by both techniques. Incubation of liver endothelium with double-labeled TF (sialic acids with 3H and protein core with 125I or 59Fe) led initially to a concordant uptake of the two labels, which were then dissociated and 3H was retained by the cell. These findings indicate desialation of TF by liver endothelium. The significance of these findings in the pathogenesis of hepatic siderosis is discussed.

Full text

PDF
508

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashwell G., Morell A. G. The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol Relat Areas Mol Biol. 1974;41(0):99–128. doi: 10.1002/9780470122860.ch3. [DOI] [PubMed] [Google Scholar]
  2. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bunn H. F., Shapiro R., McManus M., Garrick L., McDonald M. J., Gallop P. M., Gabbay K. H. Structural heterogeneity of human hemoglobin A due to nonenzymatic glycosylation. J Biol Chem. 1979 May 25;254(10):3892–3898. [PubMed] [Google Scholar]
  4. Ciechanover A., Schwartz A. L., Dautry-Varsat A., Lodish H. F. Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line. Effect of lysosomotropic agents. J Biol Chem. 1983 Aug 25;258(16):9681–9689. [PubMed] [Google Scholar]
  5. DYER J. R. Use of periodate oxidations in biochemical analysis. Methods Biochem Anal. 1956;3:111–152. doi: 10.1002/9780470110195.ch5. [DOI] [PubMed] [Google Scholar]
  6. David G. S. Solid state lactoperoxidase: a highly stable enzyme for simple, gentle iodination of proteins. Biochem Biophys Res Commun. 1972 Jul 25;48(2):464–471. doi: 10.1016/s0006-291x(72)80074-3. [DOI] [PubMed] [Google Scholar]
  7. Haigler H. T., Maxfield F. R., Willingham M. C., Pastan I. Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells. J Biol Chem. 1980 Feb 25;255(4):1239–1241. [PubMed] [Google Scholar]
  8. Huebers H. A., Finch C. A. Transferrin: physiologic behavior and clinical implications. Blood. 1984 Oct;64(4):763–767. [PubMed] [Google Scholar]
  9. Iacopetta B. J., Morgan E. H. The kinetics of transferrin endocytosis and iron uptake from transferrin in rabbit reticulocytes. J Biol Chem. 1983 Aug 10;258(15):9108–9115. [PubMed] [Google Scholar]
  10. Irie S., Tavassoli M. Liver endothelium desialates ceruloplasmin. Biochem Biophys Res Commun. 1986 Oct 15;140(1):94–100. doi: 10.1016/0006-291x(86)91062-4. [DOI] [PubMed] [Google Scholar]
  11. Karin M., Mintz B. Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells. J Biol Chem. 1981 Apr 10;256(7):3245–3252. [PubMed] [Google Scholar]
  12. Kataoka M., Tavassoli M. Ceruloplasmin receptors in liver cell suspensions are limited to the endothelium. Exp Cell Res. 1984 Nov;155(1):232–240. doi: 10.1016/0014-4827(84)90784-5. [DOI] [PubMed] [Google Scholar]
  13. Kataoka M., Tavassoli M. The role of liver endothelium in the binding and uptake of ceruloplasmin: studies with colloidal gold probe. J Ultrastruct Res. 1985 Feb;90(2):194–202. doi: 10.1016/0889-1605(85)90109-0. [DOI] [PubMed] [Google Scholar]
  14. Kishimoto T., Tavassoli M. Double labeling of transferrin: tritium labeling of sialic acid and 125I or 59Fe labeling of the protein moiety. Anal Biochem. 1986 Mar;153(2):324–329. doi: 10.1016/0003-2697(86)90099-0. [DOI] [PubMed] [Google Scholar]
  15. Kishimoto T., Tavassoli M. Endothelial binding of transferrin in fractionated liver cell suspensions. Biochim Biophys Acta. 1985 Jul 30;846(1):14–20. doi: 10.1016/0167-4889(85)90104-1. [DOI] [PubMed] [Google Scholar]
  16. Kishimoto T., Tavassoli M. Transendothelial transport (transcytosis) of iron-transferrin complex in the rat liver. Am J Anat. 1987 Mar;178(3):241–249. doi: 10.1002/aja.1001780305. [DOI] [PubMed] [Google Scholar]
  17. Klausner R. D., Van Renswoude J., Ashwell G., Kempf C., Schechter A. N., Dean A., Bridges K. R. Receptor-mediated endocytosis of transferrin in K562 cells. J Biol Chem. 1983 Apr 25;258(8):4715–4724. [PubMed] [Google Scholar]
  18. Kohno H., Tokunaga R. Transferrin and iron uptake by rat reticulocytes. J Biochem. 1985 Apr;97(4):1181–1188. doi: 10.1093/oxfordjournals.jbchem.a135163. [DOI] [PubMed] [Google Scholar]
  19. Okada S., Jarvis B., Brown E. B. In vivo evidence for the functional heterogeneity of transferrin-bound iron. V. Isotransferrins: an explanation of the Fletcher-Huehns phenomenon in the rat. J Lab Clin Med. 1979 Feb;93(2):189–198. [PubMed] [Google Scholar]
  20. Regoeczi E., Chindemi P. A., Debanne M. T. Transferrin glycans: a possible link between alcoholism and hepatic siderosis. Alcohol Clin Exp Res. 1984 May-Jun;8(3):287–292. doi: 10.1111/j.1530-0277.1984.tb05513.x. [DOI] [PubMed] [Google Scholar]
  21. Regoeczi E., Taylor P., Debanne M. T., März L., Hatton M. W. Three types of human asialo-transferrin and their interactions with the rat liver. Biochem J. 1979 Nov 15;184(2):399–407. doi: 10.1042/bj1840399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Regoeczi E., Taylor P., Hatton M. W., Wong K. L., Koj A. Distinction between binding and endocytosis of human asialo-transferrin by the rat liver. Biochem J. 1978 Jul 15;174(1):171–178. doi: 10.1042/bj1740171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schreiber G., Dryburgh H., Millership A., Matsuda Y., Inglis A., Phillips J., Edwards K., Maggs J. The synthesis and secretion of rat transferrin. J Biol Chem. 1979 Dec 10;254(23):12013–12019. [PubMed] [Google Scholar]
  24. Seglen P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 1976;13:29–83. doi: 10.1016/s0091-679x(08)61797-5. [DOI] [PubMed] [Google Scholar]
  25. Soda R., Tavassoli M. Distribution of insulin receptors in liver cell suspensions using a minibead probe. Highest density is on endothelial cell. Exp Cell Res. 1983 May;145(2):389–395. doi: 10.1016/0014-4827(83)90017-4. [DOI] [PubMed] [Google Scholar]
  26. Soda R., Tavassoli M. Insulin uptake by rat liver endothelium studied in fractionated liver cell suspensions. Mol Cell Biochem. 1985 Jan;65(2):117–123. doi: 10.1007/BF00221094. [DOI] [PubMed] [Google Scholar]
  27. Soda R., Tavassoli M., Jacobsen D. W. Receptor distribution and the endothelial uptake of transcobalamin II in liver cell suspensions. Blood. 1985 Apr;65(4):795–802. [PubMed] [Google Scholar]
  28. Soda R., Tavassoli M. Liver endothelium and not hepatocytes or Kupffer cells have transferrin receptors. Blood. 1984 Feb;63(2):270–276. [PubMed] [Google Scholar]
  29. Stibler H., Borg S. Evidence of a reduced sialic acid content in serum transferrin in male alcoholics. Alcohol Clin Exp Res. 1981 Fall;5(4):545–549. doi: 10.1111/j.1530-0277.1981.tb05358.x. [DOI] [PubMed] [Google Scholar]
  30. Storey E. L., Anderson G. J., Mack U., Powell L. W., Halliday J. W. Desialylated transferrin as a serological marker of chronic excessive alcohol ingestion. Lancet. 1987 Jun 6;1(8545):1292–1294. doi: 10.1016/s0140-6736(87)90544-7. [DOI] [PubMed] [Google Scholar]
  31. Takahashi K., Tavassoli M. Biphasic uptake of iron-transferrin complex by L1210 murine leukemia cells and rat reticulocytes. Biochim Biophys Acta. 1982 Feb 8;685(1):6–12. doi: 10.1016/0005-2736(82)90027-x. [DOI] [PubMed] [Google Scholar]
  32. Tavassoli M., Kishimoto T., Kataoka M. Liver endothelium mediates the hepatocyte's uptake of ceruloplasmin. J Cell Biol. 1986 Apr;102(4):1298–1303. doi: 10.1083/jcb.102.4.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tavassoli M., Kishimoto T., Soda R., Kataoka M., Harjes K. Liver endothelium mediates the uptake of iron-transferrin complex by hepatocytes. Exp Cell Res. 1986 Aug;165(2):369–379. doi: 10.1016/0014-4827(86)90591-4. [DOI] [PubMed] [Google Scholar]
  34. Tavassoli M. Liver endothelium binds, transports, and desialates ceruloplasmin which is then recognized by galactosyl receptors of hepatocytes. Trans Assoc Am Physicians. 1985;98:370–377. [PubMed] [Google Scholar]
  35. Van Lenten L., Ashwell G. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins. J Biol Chem. 1971 Mar 25;246(6):1889–1894. [PubMed] [Google Scholar]
  36. Zahlten R. N., Hagler H. K., Nejtek M. E., Day C. J. Morphological characterization of Kupffer and endothelial cells of rat liver isolated by counterflow elutriation. Gastroenterology. 1978 Jul;75(1):80–87. [PubMed] [Google Scholar]
  37. van Renswoude J., Bridges K. R., Harford J. B., Klausner R. D. Receptor-mediated endocytosis of transferrin and the uptake of fe in K562 cells: identification of a nonlysosomal acidic compartment. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6186–6190. doi: 10.1073/pnas.79.20.6186. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES