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. 1988 Aug;82(2):739–743. doi: 10.1172/JCI113656

Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction.

P J Newman 1, J Gorski 1, G C White 2nd 1, S Gidwitz 1, C J Cretney 1, R H Aster 1
PMCID: PMC303572  PMID: 3403726

Abstract

Human platelets are derived from megakaryocytes as anucleate cells, and thus contain only vestigial amounts of RNA capable of being transcribed into protein. This has greatly hampered efforts to study directly platelet-specific gene products and their associated polymorphisms. In this report, we describe direct amplification, using the polymerase chain reaction, of platelet-derived mRNA in amounts sufficient to permit detailed analysis, such as restriction mapping and nucleotide sequencing. The ability to generate large amounts of cDNA from platelet-specific mRNA sequences should make possible direct molecular characterization of normal platelet proteins, and facilitate the investigation of a wide variety of inherited platelet disorders.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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