Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1988 Sep;82(3):928–935. doi: 10.1172/JCI113700

Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.

D Sorrentino 1, D Stump 1, B J Potter 1, R B Robinson 1, R White 1, C L Kiang 1, P D Berk 1
PMCID: PMC303604  PMID: 3417874

Abstract

Uptake of [3H]oleate by canine or rat cardiac myocytes is saturable, displays the countertransport phenomenon, and is inhibited by phloretin and trypsin. Cardiac myocytes contain a basic (pI approximately 9.1) 40-kD plasma membrane fatty acid binding protein (FABPPM) analogous to those recently isolated from liver, adipose tissue, and gut, unrelated to the 12-14-kD cytosolic FABP in these same tissues. An antibody to rat liver FABPPM selectively inhibits specific uptake of [3H]oleate by rat heart myocytes at 37 degrees C, but has no influence on nonspecific [3H]oleate uptake at 4 degrees C or on specific uptake of [3H]glucose. Uptake of long-chain free fatty acids by cardiac muscle cells, liver, and adipose tissue and absorption by gut epithelial cells is a facilitated process mediated by identical or closely related plasma membrane FABPs.

Full text

PDF
928

Images in this article

932Image
on p.932

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abumrad N. A., Park J. H., Park C. R. Permeation of long-chain fatty acid into adipocytes. Kinetics, specificity, and evidence for involvement of a membrane protein. J Biol Chem. 1984 Jul 25;259(14):8945–8953. [PubMed] [Google Scholar]
  2. Abumrad N. A., Perkins R. C., Park J. H., Park C. R. Mechanism of long chain fatty acid permeation in the isolated adipocyte. J Biol Chem. 1981 Sep 10;256(17):9183–9191. [PubMed] [Google Scholar]
  3. Anversa P., Loud A. V., Giacomelli F., Wiener J. Absolute morphometric study of myocardial hypertrophy in experimental hypertension. II. Ultrastructure of myocytes and interstitium. Lab Invest. 1978 May;38(5):597–609. [PubMed] [Google Scholar]
  4. Bass N. M. Function and regulation of hepatic and intestinal fatty acid binding proteins. Chem Phys Lipids. 1985 Aug 30;38(1-2):95–114. doi: 10.1016/0009-3084(85)90060-x. [DOI] [PubMed] [Google Scholar]
  5. DeGrella R. F., Light R. J. Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. I. Kinetics of homologous fatty acids. J Biol Chem. 1980 Oct 25;255(20):9731–9738. [PubMed] [Google Scholar]
  6. DeGrella R. F., Light R. J. Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. II. Inhibition by albumin and fatty acid homologues, and the effect of temperature and metabolic reagents. J Biol Chem. 1980 Oct 25;255(20):9739–9745. [PubMed] [Google Scholar]
  7. Gerards P., Graf W., Kammermeier Glucose transfer studies in isolated cardiocytes of adult rats. J Mol Cell Cardiol. 1982 Mar;14(3):141–149. doi: 10.1016/0022-2828(82)90112-2. [DOI] [PubMed] [Google Scholar]
  8. Goresky C. A., Bach G. G., Wolkoff A. W., Rose C. P., Cousineau D. Sequestered tracer outflow recovery in multiple indicator dilution experiments. Hepatology. 1985 Sep-Oct;5(5):805–814. doi: 10.1002/hep.1840050516. [DOI] [PubMed] [Google Scholar]
  9. Goresky C. A., Daly D. S., Mishkin S., Arias I. M. Uptake of labeled palmitate by the intact liver: role of intracellular binding sites. Am J Physiol. 1978 Jun;234(6):E542–E553. doi: 10.1152/ajpendo.1978.234.6.E542. [DOI] [PubMed] [Google Scholar]
  10. Hewett K., Legato M. J., Danilo P., Jr, Robinson R. B. Isolated myocytes from adult canine left ventricle: Ca2+ tolerance, electrophysiology, and ultrastructure. Am J Physiol. 1983 Nov;245(5 Pt 1):H830–H839. doi: 10.1152/ajpheart.1983.245.5.H830. [DOI] [PubMed] [Google Scholar]
  11. Kuhl W. E., Spector A. A. Uptake of long-chain fatty acid methyl esters by mammalian cells. J Lipid Res. 1970 Sep;11(5):458–465. [PubMed] [Google Scholar]
  12. Neely J. R., Rovetto M. J., Oram J. F. Myocardial utilization of carbohydrate and lipids. Prog Cardiovasc Dis. 1972 Nov-Dec;15(3):289–329. doi: 10.1016/0033-0620(72)90029-1. [DOI] [PubMed] [Google Scholar]
  13. Nunn W. D., Colburn R. W., Black P. N. Transport of long-chain fatty acids in Escherichia coli. Evidence for role of fadL gene product as long-chain fatty acid receptor. J Biol Chem. 1986 Jan 5;261(1):167–171. [PubMed] [Google Scholar]
  14. Paris S., Samuel D., Romey G., Ailhaud G. Uptake of fatty acids by cultured cardiac cells from chick embryo: evidence for a facilitation process without energy dependence. Biochimie. 1979;61(3):361–367. doi: 10.1016/s0300-9084(79)80129-7. [DOI] [PubMed] [Google Scholar]
  15. Potter B. J., Stump D., Schwieterman W., Sorrentino D., Jacobs L. N., Kiang C. L., Rand J. H., Berk P. D. Isolation and partial characterization of plasma membrane fatty acid binding proteins from myocardium and adipose tissue and their relationship to analogous proteins in liver and gut. Biochem Biophys Res Commun. 1987 Nov 13;148(3):1370–1376. doi: 10.1016/s0006-291x(87)80283-8. [DOI] [PubMed] [Google Scholar]
  16. Reider H., Ramadori G., Meyer zum Büschenfelde K. H. Opsonic activity of human ascitic fluid. Hepatology. 1986 May-Jun;6(3):545–546. doi: 10.1002/hep.1840060347. [DOI] [PubMed] [Google Scholar]
  17. Rose C. P., Goresky C. A., Bach G. G. The capillary and sarcolemmal barriers in the heart. An exploration of labeled water permeability. Circ Res. 1977 Oct;41(4):515–533. doi: 10.1161/01.res.41.4.515. [DOI] [PubMed] [Google Scholar]
  18. Rose C. P., Goresky C. A. Constraints on the uptake of labeled palmitate by the heart. The barriers at the capillary and sarcolemmal surfaces and the control of intracellular sequestration. Circ Res. 1977 Oct;41(4):534–545. doi: 10.1161/01.res.41.4.534. [DOI] [PubMed] [Google Scholar]
  19. Samuel D., Paris S., Ailhaud G. Uptake and metabolism of fatty acids and analogues by cultured cardiac cells from chick embryo. Eur J Biochem. 1976 May 1;64(2):583–595. doi: 10.1111/j.1432-1033.1976.tb10338.x. [DOI] [PubMed] [Google Scholar]
  20. Scharschmidt B. F., Gollan J. L. Current concepts of bilirubin metabolism and hereditary hyperbilirubinemia. Prog Liver Dis. 1979;6:187–212. [PubMed] [Google Scholar]
  21. Schwieterman W., Sorrentino D., Potter B. J., Rand J., Kiang C. L., Stump D., Berk P. D. Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut. Proc Natl Acad Sci U S A. 1988 Jan;85(2):359–363. doi: 10.1073/pnas.85.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spector A. A., Fletcher J. E., Ashbrook J. D. Analysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constants. Biochemistry. 1971 Aug 17;10(17):3229–3232. doi: 10.1021/bi00793a011. [DOI] [PubMed] [Google Scholar]
  23. Spratt S. K., Black P. N., Ragozzino M. M., Nunn W. D. Cloning, mapping, and expression of genes involved in the fatty acid-degradative multienzyme complex of Escherichia coli. J Bacteriol. 1984 May;158(2):535–542. doi: 10.1128/jb.158.2.535-542.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stremmel W., Berk P. D. Hepatocellular influx of [14C]oleate reflects membrane transport rather than intracellular metabolism or binding. Proc Natl Acad Sci U S A. 1986 May;83(10):3086–3090. doi: 10.1073/pnas.83.10.3086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stremmel W. Fatty acid uptake by isolated rat heart myocytes represents a carrier-mediated transport process. J Clin Invest. 1988 Mar;81(3):844–852. doi: 10.1172/JCI113393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stremmel W., Lotz G., Strohmeyer G., Berk P. D. Identification, isolation, and partial characterization of a fatty acid binding protein from rat jejunal microvillous membranes. J Clin Invest. 1985 Mar;75(3):1068–1076. doi: 10.1172/JCI111769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stremmel W., Strohmeyer G., Berk P. D. Hepatocellular uptake of oleate is energy dependent, sodium linked, and inhibited by an antibody to a hepatocyte plasma membrane fatty acid binding protein. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3584–3588. doi: 10.1073/pnas.83.11.3584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stremmel W., Strohmeyer G., Borchard F., Kochwa S., Berk P. D. Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes. Proc Natl Acad Sci U S A. 1985 Jan;82(1):4–8. doi: 10.1073/pnas.82.1.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stremmel W. Translocation of fatty acids across the basolateral rat liver plasma membrane is driven by an active potential-sensitive sodium-dependent transport system. J Biol Chem. 1987 May 5;262(13):6284–6289. [PubMed] [Google Scholar]
  30. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Weisiger R. A. Dissociation from albumin: a potentially rate-limiting step in the clearance of substances by the liver. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1563–1567. doi: 10.1073/pnas.82.5.1563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Weisiger R., Gollan J., Ockner R. Receptor for albumin on the liver cell surface may mediate uptake of fatty acids and other albumin-bound substances. Science. 1981 Mar 6;211(4486):1048–1051. doi: 10.1126/science.6258226. [DOI] [PubMed] [Google Scholar]
  33. Wittenberg B. A., Robinson T. F. Oxygen requirements, morphology, cell coat and membrane permeability of calcium-tolerant myocytes from hearts of adult rats. Cell Tissue Res. 1981;216(2):231–251. doi: 10.1007/BF00233618. [DOI] [PubMed] [Google Scholar]
  34. Wosilait W. D., Nagy P. A method of computing drug distribution in plasma using stepwise association constants: clofibrate acid as an illustrative example. Comput Programs Biomed. 1976 Oct;6(3):142–148. doi: 10.1016/0010-468x(76)90020-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES