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. 2009 Dec 1;32(4):792–796. doi: 10.1590/S1415-47572009005000078

Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)

Maressa Ferreira Neto 1, Marcelo Ricardo Vicari 2,✉, Edemar Furquim de Camargo 2, Roberto Ferreira Artoni 2, Orlando Moreira-Filho 1
PMCID: PMC3036894  PMID: 21637456

Abstract

Cytogenetic data are presented for Astyanax altiparanae populations from three Brazilian hydrographic systems. The chromosomal data obtained in A. altiparanae support the hypothesis of diploid number conservation. However, small differences in the karyotype formula and number of nucleolar organizer regions were observed in these populations. The apparent karyotypical similarity among the studied populations strongly suggests a close relationship among them with some chromosomal divergences due to gene flow restriction.

Keywords: cytotaxonomy, karyotype diversification, fish cytogenetics

Astyanax is a genus from the family Characidae widely distributed throughout South and Central America and previously assigned to the subfamily Tetragonopterinae (Géry, 1977). This group has been recently considered Incertae sedis for not exhibiting consistent evidence of monophyletism (Lima et al., 2003). Astyanax altiparanae is a typical species of the upper Paraná River Basin above Sete Quedas, Brazil (Garutti and Britski, 2000), with the exception of one population from a location in the Iguaçu River situated below this region (Graça and Pavanelli, 2002).

The cytogenetics of Neotropical fishes has greatly contributed to their systematics and taxonomy. There are numerous examples among Astyanax, mainly from the “scabripinnis” and “fasciatus” groups, in which the intraspecific cytogenetic variation among populations was shown to be much larger than that detected through morphological systematics, thereby forming true species complexes (Moreira-Filho and Bertollo, 1991; Artoni et al., 2006; Vicari et al., 2008a).

The aim of this work was to characterize the karyotypes of three A. altiparanae populations and to compare them with those described in the literature. This comparative analysis could reveal population markers and help to establish cytosystematic, evolutionary and/or biogeographical relationships among the populations.

Forty-nine specimens of A. altiparanae were analyzed: nine specimens (five males and four females) from the Pântano Stream in the Tietê River Basin of the state of São Paulo; eight specimens (five males and three females) from the Feijão Stream in the Mogi-Guaçu River Basin in the state of São Paulo; and 32 specimens collected at the Salto Segredo hydroelectric power plant on the Jordão River in the Iguaçu River Basin in the state of Paraná. The procedures were approved by the Ethics Committee on Animal Experimentation of the Universidade Estadual de Ponta Grossa (Process no. 04741/08).

Chromosome preparations were obtained from anterior kidney cells using the in vivo colchicine treatment (Bertollo et al., 1978). Nucleolar organizer regions (NORs) were detected after silver nitrate staining (Ag-NOR) according to Howell and Black (1980) and C-banding was obtained as described in Sumner (1972).

Fluorescent in situ hybridization (FISH) was performed with a biotinyled 18S rDNA probe from the fish Prochilodus argenteus (Hatanaka and Galetti Jr, 2004) and with a biotinyled 5S rDNA probe from the fish Leporinus elongatus (Martins and Galetti Jr, 1999). An Olympus BX50 epifluorescence microscope was used for the analysis.

Nearly 30 metaphases were analyzed per specimen to determine the diploid number, karyotype composition and Ag-NOR distribution. Chromosomes were classified as metacentric (m), submetacentric (sm), subtelocentric (st) and acrocentric (a) following Levan et al. (1964).

Fishes of the genus Astyanax have shown considerable karyotype diversity. The diploid and fundamental numbers (2n and FN) allied to some other chromosome markers, allowed cytotaxonomic and evolutionary inferences regarding these fishes. The A. altiparanae populations studied (Pântano Stream, Feijão Stream and Jordão River) have a diploid number of 2n = 50 chromosomes (Figure 1). However, differences in the fundamental numbers were found among the three populations analyzed. The specimens from the Pântano Stream displayed a FN = 88, with 6m+28sm+4st+12a. The Feijão Stream population had a FN = 94, with 6m+30sm+8st+6a, whereas the specimens from the Jordão River showed a FN = 92, with 6m+28sm+8st+8a (Figure 1, Table 1). In the Jordão River population, chromosome pair 2 was smaller than the same pair in the Pântano and Feijão Streams populations (Figure 1). Variations in diploid number were observed between populations of species from the A. scabripinnis and A. fasciatus complexes and resulted from chromosome translocations (Mantovani et al., 2000; Artoni et al., 2006; Vicari et al., 2008a). Nevertheless, A. altiparanae exhibited a conserved 2n = 50 chromosomes in all the populations karyotyped (Table 1). Despite presenting the same 2n, different fundamental numbers were detected due to chromosome morphology variations. Non-Robertsonian rearrangements, such as pericentric inversions were suggested to explain the karyotype differences among A. altiparanae populations (Domingues et al. 2007).

Figure 1.

Figure 1

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Karyotypes of Astyanax altiparanae: population from the Pântano Stream after conventional Giemsa staining (a) and sequential C-banding (b); in the inset, the chromosomes with Ag-NORs. Population from the Feijão Stream after conventional Giemsa staining (c) and sequential C-banding (d); in the inset, the chromosomes with Ag-NORs. Population from the Jordão River after conventional Giemsa staining (e) and sequential C-banding (f); in the inset, the chromosomes with Ag-NORs. The bar represents 10 μm.

Table 1.

Chromosome numbers (2n) in Argentinean species of Orchidaceae.

Taxon 2n Locality and vouchers 2n - Previous references
Aspidogyne kuczyynskii (Porsh) Garay 2 Ctes. Ituzaingó, Santa Tecla, Almada 151 -
Brassavola tuberculata Hook 40 Mnes. Capital, Posadas, Hojsgaard 228 40 - Afzelius (1943), Blumenschein (1960)
Campylocentrum neglectum (Rchb. f. & Warm.) Cogn. 38 Chaco, San Fernando, Colonia Benítez, Insaurralde 676 38 - Dematteis and Daviña (1999)
Catasetum fimbriatum (C. Morren.) Lindl. & Paxton 108 Mnes. Capital, Posadas, Insaurralde 707 108 - Jones and Darker (1968), Dematteis and Daviña (1999)
Corymborkis flava (Sw.) Kuntze 1 56 Mnes. San Pedro, P. P. Saltos del Moconá, Daviña 208 -
Cyclopogon callophyllus (Barb. Rodr.) Barb. Rodr.1 28 Mnes. Capital, Nemesio Parma, Cerutti 74 -
28 Ctes. Ituzaingó, Garapé, Cerutti 28 -
C. congestus (Vell.) Hoehne 32 Mnes. Candelaria, Aº Yabebiry, Almada 150 28, 32, 36 - Martínez (1981), Tanaka and Maekawa (1983)
32 Mnes. Aristóbulo del Valle, Cuña Pirú, Hojsgaard 192
C. elatus (Sw.) Schltr. 28 Mnes. Apóstoles, Aº Chimiray, Hojsgaard 341 28, 30, 45 - Martínez (1981), Felix and Guerra (2005)
28 Ctes. Capital, Insaurralde 708 -
C. oliganthus (Hoehne) Hoehne & Schltr.1 64 Mnes. Apóstoles, Aº Chimiray, Hojsgaard 339 -
64 Mnes. Capital, Posadas, Aº Zaimán, Cerutti 71
Cyrtopodium hatschbachii Pabst 1 46 Mnes. Capital, Villa Lanús, Aº Zaimán, Almada 153 -
C. palmifrons Rchb. f. et Warm.1 46 Mnes. Capital, Posadas, Hojsgaard 354 -
Eltroplectris schlechteriana (Porto & Brade) Pabst 26 Mnes. Montecarlo, Honfi 1357 26 - Martínez (1985), Dematteis and Daviña (1999)
Eurystyles actinosophila (Barb. Rodr.) Schltr.2 56 Mnes. Capital, Honfi 1358 -
Galeandra beyrichii Rchb. f.1 54 Mnes. Capital, Garupá, Insaurralde 705 -
Gomesa planifolia (Lindl.) Klotzsch ex Rchb.f.1 56 Mnes. San Pedro, Piñalito, Guillén 492 -
Habenaria bractescens Lindl.1 44 Mnes. Capital, Garupá, Insaurralde 697 -
Leptotes unicolor Bar. Rodr. 40 Mnes. San Ignacio, Teyú Cuaré, Seijo 706 40 - Blumenschein (1960)
Mesadenella cuspidata (Lindl.) Garay 46 Mnes. Capital, Garupá, Hojsgaard 349 46 - Martínez (1985)
46 Ctes. Ituzaingó, Garapé, Cerutti 68
Miltonia flavescens (Lindl.) Lindl. 60 Mnes. Eldorado, Valle Hermoso, Honfi 1153 60 - Sinotô (1962, 1969), Charanasri et al. (1973), Félix and Guerra (2000)
Oeceoclades maculata (Lindl.) Lindl. 56 Mnes. San Ignacio, Dematteis 486 56 - Dematteis and Daviña (1999)
56 Mnes. Capital, Posadas, Daviña 613 48, c.52, 54, 58 - Guerra (1986), Felix and Guerra (2000)
Oncidium bifolium Sims 108 Mnes. Guaraní, Honfi 1359 108 - Dematteis and Daviña (1999)
O. divaricatum Lindl.3 56 Mnes. Bernardo de Irigoyen, Pozo Azul, Honfi 1360 42 - Charanasri et al. (1973)
O. edwallii Cogn.1 42 Mnes. Montecarlo, Puerto Rico, Insaurralde w/n -
O. fimbriatum Lindl.1 56 Mnes. Bernardo de Irigoyen, Pozo Azul, Honfi 1363 -
O. longicornu Mutel 3 42 Mnes. Iguazú, Puerto Iguazú, Daviña 615 56 - Dematteis and Daviña (1999)
O. longipes Lindl. 56 Mnes. Iguazú, Puerto Iguazú, Daviña 616 56 - Blumenschein (1960), Dematteis and Daviña (1999)
O. pubes Lindl.1 84 Mnes. Guaraní, Cabassi w/n -
O. riograndense Cogn.1 56 Mnes. Bernardo de Irigoyen, Pozo Azul, Honfi 1192 -
Pelexia bonariensis (Lindl.) Schltr. 46 Mnes. Capital, Nemesio Parma, Cerutti 29 46 - Martínez (1985), Dematteis and Daviña (1999)
46 Mnes. Candelaria, P. P. Cañadón de Profundidad, Hojsgaard 289
P. ekmanii (Kraenzl) Schltr.1 46 Mnes. Capital, Garupá, Radins 15 -
P. lindmanii Kraenzl 1. 46 Mnes. San Pedro, P. P. Saltos del Moconá, Daviña 123 -
Rodriguezia decora (Lem.) Rchb. f. 42 Mnes. Bernardo de Irigoyen, Pozo Azul, Honfi 1362 Sinoto (1962)
Sacoila lanceolata (Aubl.) Garay 46 Mnes. Capital, Garupá, Radins 55 46 - Cocucci (1956), Martínez (1985), Felix and Guerra (2005)
Sarcoglottis fasciculata (Vell.) Schltr.3 46, 47, 49 Mnes. Capital, Nemesio Parma, Hojsgaard 291B 46 - Martínez (1985), Felix and Guerra (2005)
46 Mnes. Capital, Garupá, Insaurralde w/n
46 Ctes. Ituzaingó, Garapé, Insaurralde w/n
S. grandiflora (Hook.) Klotzsch 46 Mnes. Capital, Nemesio Parma, Cerutti 56 46 - Martínez (1985)
46 Ctes. Ituzaingó, Garapé, Insaurralde w/n
S. ventricosa (Vell.) Hoehne 46 Mnes. Montecarlo, Isla Caraguatay, Hojsgaard 255 46 - Martínez (1985)
Skeptrostachys paraguayensis (Rchb. f.) Garay 2 46 Mnes. Apóstoles, San José, Baumgratz 19 -
Sophronitis cernua Lindl. 40 Mnes. Capital, Posadas, Honfi 1361 40 - Blumenschein (1960)
Stigmatosema polyaden (Vell.) Garay 2 40 Mnes. Apóstoles, San José, Hadad 18 -
Trichocentrum pumilum (Lindl.) M.W.Chase & N.H.Williams 30 Mnes. Capital, Villa Lanús, Cerutti 73 30 - Dematteis (1997), Dematteis and Daviña (1999), Felix and Guerra (2000)
Warrea warreana (Lodd. ex Lindl.) C. Schweinf.1 48 Mnes. San Pedro, P. P. Saltos del Moconá, Insaurralde w/n -
Zygopetalum maxillare Lodd. 48 Mnes. Capital, Posadas, Daviña 614 48 - Blumenschein (1960), Tanaka and Kamemoto (1984)
Zygostates alleniana Kraenzl 2 54 Mnes. Apostoles, Aº Chimiray, Hojsgaard 343 -
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1First chromosome number for the species, 2First chromosome number for the genus, 3Taxa with chromosome numbers that differ from previously published reports.

Species are alphabetically grouped. All voucher specimens are deposited at MNES (Universidad Nacional de Misiones Herbarium). Mnes: Misiones, Ctes: Corrientes.

After C-banding, heterochromatic blocks were observed in the centromeric or pericentromeric regions of all chromosome pairs and in the telomeric regions of some chromosomes with small differences among the analyzed populations (Figure 1b, d and f). This results are similar to findings previously described for other A. altiparanae populations (Fernandes and Martins-Santos, 2004; Domingues et al., 2007). The small variations in constitutive heterochromatin distribution combined with the large variation in FN found among the A. altiparanae populations (Table 1) supports the hypothesis that this structural variation is not due to the amplification of heterochromatic sequences. Thus, the different FNs observed in A. altiparanae populations, sometimes even within the same hydrographic basin, such as the Tibagi, Paranapanema and Mogi-Guaçu Rivers, must be mainly due to pericentric inversions.

Silver-nitrate staining (Ag-NORs) and FISH with the 18S rDNA probe evidenced a single NOR-bearing pair in the population of the Pântano Stream, while the populations of the Feijão Stream and the Jordão River exhibited multiple terminal signals on different chromosomes (Figure 1, in the insets; and Figure 2a, c and e). Chromosome rearrangements and transference of ribosomal sites could cause these variations, but transposition events have been suggested as the main mechanism to explain the majority of cases of NORs variability in the genome of these animals (Mantovani et al., 2000; Vicari et al., 2008b). Together with variations in the fundamental number, Ag-NORs are important cytogenetic markers to determine differences among populations in this species (Pacheco et al., 2001; Fernandes and Martins-Santos, 2004; 2006). Domingues et al. (2007) carried out a comparative cytogenetic and morphometric study on A. altiparanae populations from the upper Tibagi and Iguaçu Rivers. These authors found a similar karyotype macrostructure in the populations with differences in the number of major rDNA sites between populations. The Ag-NORs and 18S rDNA FISH data (Table 1) also revealed variations between populations of this species both within the same and from different hydrographic basins.

Figure 2.

Figure 2

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Chromosome spreads of Astyanax altiparanae after FISH: (a) 18S and (b) 5S rDNA sites in the population from the Pântano Stream (c) 18S and (d) 5S rDNA sites in the Feijão Stream population; and (e) 18S and (f) 5S rDNA sites in the Jordão River population. The bar represents 10 μm.

While variations in the number of 18S rDNA sites occurred between populations of A.altiparanae, only one signal in the proximal long arm of a sm chromosome was observed after FISH with the 5S rDNA probe (Figure 2b, d and e) (Fernandes and Martins-Santos, 2004; 2006; Domingues et al., 2007; Peres et al., 2008; present study). The 5S rDNA sites seemed to be conserved in the proximal region of the long arm of two chromosome pairs (one m and one a) in populations of the A. scabripinnis complex (Ferro et al., 2001; Almeida-Toledo et al., 2002; Mantovani et al., 2005; Vicari et al., 2008a). In Astyanax sp. D and in A. janeiroensis, the 5S rDNA site was located in the proximal region of the long arm of a single acrocentric pair, similar to what was found in the A. scabripinnis complex (Kantek et al., 2007; Vicari et al., 2008b, respectively). Mantovani et al. (2000) proposed that 5S rDNA sites in the proximal position of the long arm indicated a conserved pattern for these genes in Astyanax. Thus, the chromosomes with 5S rDNA sites would be the same among the A. altiparanae populations studied, but would diverge in morphology and number of 5S rDNA sites in other populations of the genus.

The cytogenetic data from the three A. altiparanae populations studied herein are similar to those from other populations of this species reported in the literature. This observation strengthens the hypothesis of a conserved 2n = 50. Nevertheless, differences in the karyotypic formula and in the number of nucleolar organizer regions were noticed when comparing the data from these populations to those already described for other populations. The apparent karyotype similarity strongly suggests a close relationship among the studied populations, but the small karyotypic differences detected indicate some evolutionary divergence due to gene flow restrictions.

Acknowledgments

The authors are grateful to the IAP (Instituto Ambiental do Paraná) and IBAMA (Instituto Brasileiro do Meio Ambiente) for authorizing the capture of specimens (IBAMA/MMA/SISBIO license number: 15117). This work was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico Processo: 473448/2007-6), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná, Processo: 12938). We also thank Mr. Miguel Airton Carvalho for his collaboration in the field work and in the laboratory.

Footnotes

Associate Editor: Fausto Foresti

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