Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1989 Feb;83(2):616–622. doi: 10.1172/JCI113925

Basolateral membrane Na+/H+ antiport, Na+/base cotransport, and Na+-independent Cl-/base exchange in the rabbit S3 proximal tubule.

I Kurtz 1
PMCID: PMC303722  PMID: 2536402

Abstract

The basolateral membrane Na+ and Cl(-)-dependent acid-base transport processes were studied in the isolated perfused rabbit S3 proximal straight tubule. Intracellular pH (pHi) was measured with 2'7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and a microfluorometer coupled to the tubule perfusion apparatus. Reduction of basolateral HCO3- from 25 to 5 mM caused pHi to decrease at a rate of 0.81 pH/min. Approximately 50% of this rate was Na+-dependent, 30% Cl(-)-dependent and 20% Na+ and Cl(-)-independent. Two basolateral Na+-dependent acid base transport pathways were detected: (a) an amiloride-sensitive Na+/H+ antiporter and (b) a stilbene-sensitive Na+/base cotransporter. No evidence was found for a Na+-dependent Cl-/base exchanger. The Cl(-)-dependent component of basolateral base efflux was mediated by a stilbene-sensitive Na+-independent Cl-/base exchange pathway. The results suggest that the acid base transport pathways of the basolateral membrane of the S3 proximal tubule differ from more proximal nephron segments.

Full text

PDF
616

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alpern R. J., Chambers M. Basolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes. J Gen Physiol. 1987 Apr;89(4):581–598. doi: 10.1085/jgp.89.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alpern R. J., Chambers M. Cell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration. J Clin Invest. 1986 Aug;78(2):502–510. doi: 10.1172/JCI112602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alpern R. J. Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process. J Gen Physiol. 1985 Nov;86(5):613–636. doi: 10.1085/jgp.86.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biagi B. A. Effects of the anion transport inhibitor, SITS, on the proximal straight tubule of the rabbit perfused in vitro. J Membr Biol. 1985;88(1):25–31. doi: 10.1007/BF01871210. [DOI] [PubMed] [Google Scholar]
  5. Boron W. F., Boulpaep E. L. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. J Gen Physiol. 1983 Jan;81(1):53–94. doi: 10.1085/jgp.81.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boron W. F., Boulpaep E. L. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. J Gen Physiol. 1983 Jan;81(1):29–52. doi: 10.1085/jgp.81.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garvin J. L., Burg M. B., Knepper M. A. Ammonium replaces potassium in supporting sodium transport by the Na-K-ATPase of renal proximal straight tubules. Am J Physiol. 1985 Nov;249(5 Pt 2):F785–F788. doi: 10.1152/ajprenal.1985.249.5.F785. [DOI] [PubMed] [Google Scholar]
  8. Garvin J. L., Burg M. B., Knepper M. A. NH3 and NH4+ transport by rabbit renal proximal straight tubules. Am J Physiol. 1987 Feb;252(2 Pt 2):F232–F239. doi: 10.1152/ajprenal.1987.252.2.F232. [DOI] [PubMed] [Google Scholar]
  9. Golchini K., Kurtz I. NH3 permeation through the apical membrane of MDCK cells is via a lipid pathway. Am J Physiol. 1988 Jul;255(1 Pt 2):F135–F141. doi: 10.1152/ajprenal.1988.255.1.F135. [DOI] [PubMed] [Google Scholar]
  10. Grassl S. M., Aronson P. S. Na+/HCO3-co-transport in basolateral membrane vesicles isolated from rabbit renal cortex. J Biol Chem. 1986 Jul 5;261(19):8778–8783. [PubMed] [Google Scholar]
  11. Ives H. E., Yee V. J., Warnock D. G. Asymmetric distribution of the Na+/H+ antiporter in the renal proximal tubule epithelial cell. J Biol Chem. 1983 Nov 25;258(22):13513–13516. [PubMed] [Google Scholar]
  12. Kinsella J. L., Aronson P. S. Interaction of NH4+ and Li+ with the renal microvillus membrane Na+-H+ exchanger. Am J Physiol. 1981 Nov;241(5):C220–C226. doi: 10.1152/ajpcell.1981.241.5.C220. [DOI] [PubMed] [Google Scholar]
  13. Kurtz I. Apical Na+/H+ antiporter and glycolysis-dependent H+-ATPase regulate intracellular pH in the rabbit S3 proximal tubule. J Clin Invest. 1987 Oct;80(4):928–935. doi: 10.1172/JCI113184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kurtz I., Balaban R. S. Ammonium as a substrate for Na+-K+-ATPase in rabbit proximal tubules. Am J Physiol. 1986 Mar;250(3 Pt 2):F497–F502. doi: 10.1152/ajprenal.1986.250.3.F497. [DOI] [PubMed] [Google Scholar]
  15. Kurtz I., Golchini K. Na+-independent Cl(-)-HCO-3- exchange in Madin-Darby canine kidney cells. Role in intracellular pH regulation. J Biol Chem. 1987 Apr 5;262(10):4516–4520. [PubMed] [Google Scholar]
  16. Kurtz I., Star R., Balaban R. S., Garvin J. L., Knepper M. A. Spontaneous luminal disequilibrium pH in S3 proximal tubules. Role in ammonia and bicarbonate transport. J Clin Invest. 1986 Oct;78(4):989–996. doi: 10.1172/JCI112690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mahnensmith R. L., Aronson P. S. The plasma membrane sodium-hydrogen exchanger and its role in physiological and pathophysiological processes. Circ Res. 1985 Jun;56(6):773–788. doi: 10.1161/01.res.56.6.773. [DOI] [PubMed] [Google Scholar]
  18. Olsnes S., Tønnessen T. I., Sandvig K. pH-regulated anion antiport in nucleated mammalian cells. J Cell Biol. 1986 Mar;102(3):967–971. doi: 10.1083/jcb.102.3.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sabolić I., Burckhardt G. Proton pathways in rat renal brush-border and basolateral membranes. Biochim Biophys Acta. 1983 Oct 12;734(2):210–220. doi: 10.1016/0005-2736(83)90119-0. [DOI] [PubMed] [Google Scholar]
  20. Sasaki S., Shigai T., Takeuchi J. Intracellular pH in the isolated perfused rabbit proximal straight tubule. Am J Physiol. 1985 Sep;249(3 Pt 2):F417–F423. doi: 10.1152/ajprenal.1985.249.3.F417. [DOI] [PubMed] [Google Scholar]
  21. Yoshitomi K., Burckhardt B. C., Frömter E. Rheogenic sodium-bicarbonate cotransport in the peritubular cell membrane of rat renal proximal tubule. Pflugers Arch. 1985 Dec;405(4):360–366. doi: 10.1007/BF00595689. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES